FastQCFastQC Report
Thu 26 May 2016
SRR1511996_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511996_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208111
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38080.1724550984982186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCGC504.7262365E-617.0374156
GAACCGC400.005410027714.1978466
CCGCGCG500.001541909513.2513239
GGTATCA10450.012.3860381
GTCAACG656.1517046E-412.0684472
GTCCTAA3800.011.6119111
GTATCAA22900.011.0901921
TCGTGCA803.9019916E-410.6483849
CCCGTTT909.878723E-510.51692212
GGACCGA750.002730099510.0962466
TTATACT2350.010.0693934
CACTGCG951.706505E-49.96347
TAGGACC3900.09.9506274
GTTTAGA2103.6379788E-119.80561351
GTCCTAC8500.09.5748931
GTGCGCC1502.7938586E-79.46523111
CTTATAC1901.1241355E-99.465233
AAGGCGT1901.1241355E-99.465236
GTATAAC1906.148184E-99.2895291
GTAGGAC9300.09.15993
Produced by FastQC (version 0.11.5)