##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511996_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2208111 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.432170303032773 31.0 30.0 34.0 25.0 34.0 2 26.490087228404732 31.0 25.0 34.0 10.0 34.0 3 28.448917649520336 31.0 28.0 34.0 16.0 34.0 4 32.95550087835258 35.0 33.0 37.0 27.0 37.0 5 33.40553894256222 35.0 35.0 37.0 28.0 37.0 6 33.68840787442298 37.0 35.0 37.0 29.0 37.0 7 33.75809594716932 37.0 35.0 37.0 29.0 37.0 8 33.79852099826503 37.0 35.0 37.0 29.0 37.0 9 35.41654518273764 39.0 37.0 39.0 30.0 39.0 10 35.332734631547055 39.0 35.0 39.0 30.0 39.0 11 35.34214040870228 39.0 37.0 39.0 30.0 39.0 12 35.236268466576185 39.0 35.0 39.0 29.0 39.0 13 35.231321704388954 39.0 35.0 39.0 29.0 39.0 14 36.429347528271904 40.0 37.0 41.0 29.0 41.0 15 36.363503918054846 40.0 37.0 41.0 29.0 41.0 16 36.31155091388069 40.0 37.0 41.0 29.0 41.0 17 36.27101490821793 40.0 37.0 41.0 28.0 41.0 18 36.21749268945266 40.0 37.0 41.0 28.0 41.0 19 36.24316078313092 40.0 37.0 41.0 28.0 41.0 20 36.13745595216907 40.0 37.0 41.0 27.0 41.0 21 36.08449031774218 40.0 37.0 41.0 27.0 41.0 22 35.97792094690892 40.0 37.0 41.0 27.0 41.0 23 35.88500079932576 40.0 36.0 41.0 27.0 41.0 24 35.780617459901244 40.0 36.0 41.0 27.0 41.0 25 35.68048798271464 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2148046865797717 1101 2 3.9939025786232456 1101 3 2.2122506937585698 1101 4 1.2604503567705407 1101 5 0.8716428673136889 1101 6 0.5548140117069735 1101 7 0.1784658101198957 1101 8 0.2093401280582725 1101 9 0.11826674363335599 1101 10 0.35037700497066027 1101 11 0.35357988040897226 1101 12 0.3438859718087457 1101 13 0.3618918076921318 1101 14 0.2804996717160293 1101 15 0.2520699955614063 1101 16 0.5479217634219822 1101 17 0.48559484714192536 1101 18 0.5528246778017447 1101 19 0.3790608499372681 1101 20 0.40969838818587334 1101 21 0.38313527708526607 1101 22 0.5528571569783054 1101 23 0.540817595648285 1101 24 0.4288117116695531 1101 25 0.3152332826386868 1102 1 1.8237269791254818 1102 2 4.488583109063271 1102 3 2.721383203302473 1102 4 1.8539125327801855 1102 5 1.3492533756520402 1102 6 1.2057746807847352 1102 7 1.0089980082312024 1102 8 0.9168205379437495 1102 9 1.0888577120882488 1102 10 0.8870022310104488 1102 11 1.0249833363061 1102 12 0.9544643824979175 1102 13 0.9884479660204448 1102 14 1.3003251187724914 1102 15 1.239419804683365 1102 16 1.1841398666970449 1102 17 1.1576643669329627 1102 18 1.2628518025757103 1102 19 1.2811755776849054 1102 20 1.259911619047834 1102 21 1.2793815097555523 1102 22 1.1784915753025942 1102 23 1.3022941377419173 1102 24 1.3390407653163265 1102 25 1.4031203129782526 1103 1 1.4565835748358538 1103 2 4.1797072365081505 1103 3 2.46944947141591 1103 4 1.6739825963643966 1103 5 1.4078196783221202 1103 6 1.1598381094943875 1103 7 0.9756558917522966 1103 8 0.7966152715120671 1103 9 1.0091708240287218 1103 10 0.8657693365464212 1103 11 1.0935062703950749 1103 12 0.8695234455522609 1103 13 0.8416801635922653 1103 14 0.997056780716413 1103 15 1.2574122943739638 1103 16 0.9445687721731204 1103 17 1.0910449276720158 1103 18 1.1761405578455921 1103 19 1.1997745363782002 1103 20 1.2286270624095081 1103 21 1.0948015456944518 1103 22 1.123870488841888 1103 23 1.2627260669238467 1103 24 1.335982502824102 1103 25 1.3957503899243662 1104 1 1.1907131430460574 1104 2 3.7987179995213545 1104 3 2.141109046745047 1104 4 1.5196795525158322 1104 5 1.0273080259025136 1104 6 0.7868277769281349 1104 7 0.6143547738778921 1104 8 0.6796079723091282 1104 9 0.7069453305323279 1104 10 0.6146808024390182 1104 11 0.47622511311821825 1104 12 0.5639251978760313 1104 13 0.451473797392282 1104 14 0.5405836588171482 1104 15 0.5079257495201048 1104 16 0.5375747450843988 1104 17 0.5771752004171802 1104 18 0.655097864498579 1104 19 0.7278455638035624 1104 20 0.6883529100128953 1104 21 0.5671214156038218 1104 22 0.5804583506058734 1104 23 0.807622235394156 1104 24 0.7878004973460264 1104 25 0.8299584595169662 1105 1 1.2000122768541495 1105 2 3.9052168896473276 1105 3 2.180303005109014 1105 4 1.1796738455926032 1105 5 0.7496688364956796 1105 6 0.4572792190641337 1105 7 0.41497395754279154 1105 8 0.29356350032207246 1105 9 0.13497770244166674 1105 10 0.07002150225485337 1105 11 0.09984302235363174 1105 12 0.20793660472013897 1105 13 0.19565221051750825 1105 14 0.19674766108591513 1105 15 0.22723003006264975 1105 16 0.2889752170434505 1105 17 0.3636408643459248 1105 18 0.23690468339654558 1105 19 0.2915127696519306 1105 20 0.23852744383079028 1105 21 0.24440716089998915 1105 22 0.36105866291111965 1105 23 0.21657930392625246 1105 24 0.22137436173832725 1105 25 0.40162673672638505 1106 1 0.6069611061096118 1106 2 2.3211332343765534 1106 3 1.1589876310384781 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0.7150073306242462 1107 16 0.879828772412452 1107 17 0.8502508832827331 1107 18 0.8669658863717729 1107 19 0.8451654256853516 1107 20 0.8588778398117611 1107 21 0.9881249894795943 1107 22 1.0549323459571482 1107 23 1.1503313944584974 1107 24 1.2572428518046337 1107 25 1.0135881603056376 1108 1 0.9303255701606723 1108 2 3.0491785035942733 1108 3 1.77627781774045 1108 4 0.9825242435617341 1108 5 0.7046689211957116 1108 6 0.46627738712351174 1108 7 0.23043739980035838 1108 8 0.2604004140466216 1108 9 0.21968559624234985 1108 10 0.3286536451897817 1108 11 0.2851334103523655 1108 12 0.11748544396674276 1108 13 0.1526064458819505 1108 14 -0.03558875290846686 1108 15 0.19376141868332297 1108 16 0.2556088506988843 1108 17 0.22093220582497963 1108 18 0.06363867448609994 1108 19 0.2301905538023803 1108 20 0.3027808856769596 1108 21 0.21097605953341514 1108 22 0.3319980830305198 1108 23 0.3828109754450182 1108 24 0.21769354048684164 1108 25 0.2812014457817895 1109 1 1.0291934734907606 1109 2 3.1288607205269656 1109 3 1.9018115285862045 1109 4 1.1996333546378466 1109 5 0.7799760768588158 1109 6 0.5447797400590701 1109 7 0.40375404210605836 1109 8 0.4435139479146102 1109 9 0.36785665100441634 1109 10 0.2806489499662632 1109 11 0.41842756786851965 1109 12 0.2808620028760487 1109 13 0.3332736023381173 1109 14 0.3064425664500803 1109 15 0.42150711292322995 1109 16 0.43820707754338883 1109 17 0.41647901321617553 1109 18 0.40542454835465946 1109 19 0.42555782066312275 1109 20 0.39279365912288 1109 21 0.4899076942444367 1109 22 0.49550700259673164 1109 23 0.557522636657545 1109 24 0.5348157224072949 1109 25 0.6110041825081822 1110 1 0.6548883756089587 1110 2 3.3079157717057903 1110 3 1.5479397648942452 1110 4 0.7821153279827726 1110 5 0.3139081342691057 1110 6 0.17884471865903606 1110 7 -0.05981971838996003 1110 8 -0.3172209659817682 1110 9 -0.41738604128164525 1110 10 -0.38531919756098176 1110 11 -0.2987614198123083 1110 12 -0.47723323786478034 1110 13 -0.39644636729973826 1110 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-1.188431031183768 2315 17 -1.2403685001081683 2315 18 -1.2028157942902382 2315 19 -1.301935640756895 2315 20 -1.389008589531464 2315 21 -1.3937987879082172 2315 22 -1.6807956595011717 2315 23 -1.5424233291346425 2315 24 -1.3727316220758894 2315 25 -1.391379809259476 2316 1 -0.3387236860185645 2316 2 -0.01114427918738059 2316 3 0.11605382248174578 2316 4 -0.16935659726635066 2316 5 -0.5463731734409691 2316 6 -0.49908821911738244 2316 7 -0.6672360071323666 2316 8 -0.8065021485055155 2316 9 -0.8574776633289005 2316 10 -0.987555043215508 2316 11 -1.0676738912681856 2316 12 -0.8625646574507968 2316 13 -1.051682486930055 2316 14 -1.1812565946317122 2316 15 -1.0522075085811622 2316 16 -1.1729904689087647 2316 17 -1.2630006053908645 2316 18 -1.1893853310083387 2316 19 -1.0921000277850723 2316 20 -1.1616926474333695 2316 21 -1.2233993447427594 2316 22 -1.2782350659489268 2316 23 -1.3001744296225226 2316 24 -1.358111193195164 2316 25 -1.3008306612688827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75938.0 3 3.0 4 648.0 5 2984.0 6 3546.0 7 3257.0 8 3023.0 9 2969.0 10 2923.0 11 2812.0 12 2787.0 13 2770.0 14 2876.0 15 2894.0 16 3175.0 17 3463.0 18 3838.0 19 4699.0 20 6150.0 21 7842.0 22 9757.0 23 11614.0 24 13612.0 25 15302.0 26 17299.0 27 19905.0 28 22731.0 29 27746.0 30 34632.0 31 44181.0 32 57712.0 33 77224.0 34 105237.0 35 154759.0 36 249874.0 37 346966.0 38 860963.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.520728803941466 18.322765476916693 12.803251285827569 25.353254433314266 2 17.594176013541883 21.319836487742442 37.87130048356503 23.21468701515064 3 18.63307596402536 24.31218358135076 29.570116719675777 27.484623734948105 4 12.644654186315815 16.66999530367812 37.78578160246473 32.89956890754133 5 13.756192510249713 37.13626715323641 35.36774192964031 13.739798406873568 6 32.07838377617057 36.74921086358922 17.94195035573409 13.230455004506117 7 28.269140455348484 31.47663319461748 22.106814376632332 18.1474119734017 8 25.7508004583105 35.814474822359394 20.4026520417914 18.03207267753871 9 26.44645128800137 15.64948501230237 19.940709502375558 37.9633541973207 10 15.123263898323813 28.739445715517537 34.005356622049284 22.131933764109366 11 34.6026988679464 22.676260387272198 23.872894070995525 18.848146673785873 12 23.153274450423915 24.55370223688936 31.255448661774704 21.037574650912024 13 28.76010309264344 21.51993264831342 25.824607549167595 23.895356709875546 14 21.66984434652259 21.168646489531774 27.389124636000922 29.772384527944713 15 23.48948943237002 29.234807489297417 23.517748881283595 23.75795419704897 16 23.128456857467764 27.8976464498388 26.292790534533815 22.681106158159622 17 22.486505433830093 27.773286759587716 27.030072310676413 22.710135495905774 18 22.350909625303483 27.472672293507514 28.178435193584367 21.997982887604632 19 23.65673987295854 26.925208935472973 27.174563810120745 22.243487381447743 20 23.76252375908189 26.848792832580727 27.438439239473883 21.9502441688635 21 23.80431558233641 26.359743574066652 26.88178909397227 22.954151749624668 22 23.647969015432956 26.802719301542933 27.11199433712836 22.437317345895753 23 23.443204863561064 26.64247989442479 26.986939867596217 22.927375374417938 24 23.323193444532453 26.621397203310888 27.37462020704575 22.68078914511091 25 23.52083372960126 26.571643882393115 27.166725087880906 22.740797300124722 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 212.0 1 212.0 2 380.5 3 549.0 4 549.0 5 549.0 6 1293.5 7 2038.0 8 2038.0 9 2038.0 10 3102.5 11 4167.0 12 4167.0 13 4167.0 14 6969.5 15 9772.0 16 9772.0 17 9772.0 18 17282.0 19 24792.0 20 24792.0 21 24792.0 22 37524.5 23 50257.0 24 50257.0 25 50257.0 26 70662.0 27 91067.0 28 91067.0 29 91067.0 30 116697.5 31 142328.0 32 142328.0 33 142328.0 34 165153.5 35 187979.0 36 187979.0 37 187979.0 38 209421.5 39 230864.0 40 230864.0 41 230864.0 42 249004.5 43 267145.0 44 267145.0 45 267145.0 46 276849.0 47 286553.0 48 286553.0 49 286553.0 50 285134.5 51 283716.0 52 283716.0 53 283716.0 54 260005.5 55 236295.0 56 236295.0 57 236295.0 58 204922.0 59 173549.0 60 173549.0 61 173549.0 62 142190.5 63 110832.0 64 110832.0 65 110832.0 66 86194.5 67 61557.0 68 61557.0 69 61557.0 70 44717.0 71 27877.0 72 27877.0 73 27877.0 74 19388.5 75 10900.0 76 10900.0 77 10900.0 78 7371.5 79 3843.0 80 3843.0 81 3843.0 82 2550.5 83 1258.0 84 1258.0 85 1258.0 86 820.0 87 382.0 88 382.0 89 382.0 90 253.0 91 124.0 92 124.0 93 124.0 94 83.5 95 43.0 96 43.0 97 43.0 98 27.5 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.47129288337407 3 0.0 4 0.0 5 0.0 6 4.528757838713724E-5 7 0.0 8 4.528757838713724E-5 9 0.0 10 1.3586273516141172E-4 11 0.0 12 0.0 13 0.0 14 4.528757838713724E-5 15 0.0 16 0.0 17 0.0 18 1.8115031354854895E-4 19 5.887385190327841E-4 20 3.623006270970979E-4 21 0.002988980173551058 22 0.0019926534490340387 23 7.698888325813331E-4 24 0.0 25 4.075882054842352E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2208111.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.55274720067499 #Duplication Level Percentage of deduplicated Percentage of total 1 80.91737890321413 57.89860756802913 2 12.541088400458749 17.946986558786847 3 3.496443422802445 7.505403969997383 4 1.3187451960855592 3.7743936655045838 5 0.6098231265487806 2.181726000553507 6 0.3372606648962248 1.447915625763068 7 0.18223003679367358 0.9127341828547199 8 0.1262075286703809 0.7224396315018964 9 0.09620443198964906 0.6195322261565901 >10 0.3527852491024697 4.090796448448426 >50 0.012681864439479778 0.6118108012150191 >100 0.007681175392871116 1.0915909860427142 >500 8.30869342293222E-4 0.4340379147691907 >1k 6.391302633023384E-4 0.7620244203769704 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3808 0.1724550984982186 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 4.528757838713724E-5 0.0 9.057515677427448E-5 0.0 5 0.0 9.057515677427448E-5 0.0 9.057515677427448E-5 0.0 6 0.0 9.057515677427448E-5 0.0 9.057515677427448E-5 0.0 7 0.0 1.3586273516141172E-4 0.0 9.057515677427448E-5 0.0 8 0.0 1.3586273516141172E-4 0.0 9.057515677427448E-5 0.0 9 0.0 1.3586273516141172E-4 0.0 1.8115031354854895E-4 0.0 10 0.0 1.3586273516141172E-4 0.0 1.8115031354854895E-4 0.0 11 0.0 1.3586273516141172E-4 0.0 1.8115031354854895E-4 0.0 12 0.0 1.3586273516141172E-4 0.0 1.8115031354854895E-4 0.0 13 0.0 1.3586273516141172E-4 0.0 1.8115031354854895E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCCGC 50 4.7262365E-6 17.037415 6 GAACCGC 40 0.0054100277 14.197846 6 CCGCGCG 50 0.0015419095 13.251323 9 GGTATCA 1045 0.0 12.386038 1 GTCAACG 65 6.1517046E-4 12.068447 2 GTCCTAA 380 0.0 11.611911 1 GTATCAA 2290 0.0 11.090192 1 TCGTGCA 80 3.9019916E-4 10.648384 9 CCCGTTT 90 9.878723E-5 10.516922 12 GGACCGA 75 0.0027300995 10.096246 6 TTATACT 235 0.0 10.069393 4 CACTGCG 95 1.706505E-4 9.9634 7 TAGGACC 390 0.0 9.950627 4 GTTTAGA 210 3.6379788E-11 9.8056135 1 GTCCTAC 850 0.0 9.574893 1 GTGCGCC 150 2.7938586E-7 9.465231 11 CTTATAC 190 1.1241355E-9 9.46523 3 AAGGCGT 190 1.1241355E-9 9.46523 6 GTATAAC 190 6.148184E-9 9.289529 1 GTAGGAC 930 0.0 9.1599 3 >>END_MODULE