FastQCFastQC Report
Thu 26 May 2016
SRR1511996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208111
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46710.21153827864631805No Hit
TATCAACGCAGAGTACTTTTTTTTT27900.1263523437001129No Hit
GGTATCAACGCAGAGTACTTTTTTT27590.12494842877011166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG250.006016844419.0087475
GGTATCA10150.012.6976981
CCGACCC604.1118014E-412.6598749
CGGGACT700.001486113810.8621434
TGGTATC3800.010.7549512
TAGGACC3400.010.6225374
GTATTAA2800.010.5696571
GTATCAA23350.010.50761
TCGAACT1553.6907295E-910.41494619
AGGACGT8800.010.2604055
TAGGACG9300.010.2197574
CGTGCAC1409.7692464E-810.17311210
GGACGTG8600.010.1628646
CTTACAC2900.010.1598483
TTACACC2250.010.13800054
CCGTATG750.00266013710.1278989
GGGTAAT1159.68518E-69.9618651
GTAGGAC9650.09.9475843
GACGTGG4600.09.9077277
TGGCGAG7850.09.79751318
Produced by FastQC (version 0.11.5)