FastQCFastQC Report
Thu 26 May 2016
SRR1511995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230105
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30540.13694422459929018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9200.012.2475771
TAGACTC1701.8189894E-1211.6931835
AGGACGT6550.010.5497925
GTCCTAG3000.010.4512661
GTATCAA19850.010.4150691
GGACGTG6300.010.3674256
TAGGACG7150.010.19405654
GGATATA1604.1189196E-89.7980621
GCCCTAT1201.1793232E-59.7980611
TTAGGAC4950.09.7527553
GTATTAG1656.6243956E-89.5011511
GTCCTAA4650.09.4819961
AGGGTCG900.00115113869.465915
TTTATAC2900.09.465913
CTAATAC1804.451067E-99.465913
GTATAGG2600.09.4212131
GCCTTAC1251.9127769E-59.406141
GTCTAAT1251.9127769E-59.406141
GTCAACG1157.930471E-59.3720592
CCTACAC2750.09.2938023
Produced by FastQC (version 0.11.5)