FastQCFastQC Report
Thu 26 May 2016
SRR1511995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230105
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36970.16577694772219245No Hit
TATCAACGCAGAGTACTTTTTTTTT22380.10035401920537373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATC604.1097632E-412.66062813
GGTATCA10750.012.3415071
GTACTAG855.0764123E-511.2290481
GGATCGT600.005881199611.08227356
AGGACCG1252.2310815E-710.64351755
ACCGTCC909.5645344E-510.5505248
GTATCAA21600.010.3400811
TAGGACG6250.010.1873674
TATTAGG1409.646283E-810.1819382
TAGGACC3200.010.0970884
AGGACGT5750.010.08159355
TAAGACC2201.8189894E-129.9351024
GGACGTG6050.09.8916166
TTAGGAC5550.09.7599833
GTAGGAC7150.09.7025083
TAGACAG3250.09.64934355
TATTATA3250.09.64934352
TGGTATC4250.09.391342
TTAAGAC2650.09.3238373
TAGACTG2451.8189894E-129.30925
Produced by FastQC (version 0.11.5)