FastQCFastQC Report
Thu 26 May 2016
SRR1511994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283455
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30320.13278124596280635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG802.0793523E-512.2534981
GGTATCA9200.011.401081
TAGGACC4100.011.08140854
GCTAACC600.006038998711.04293253
GTCTTAC1707.8216544E-1110.9560691
TGGCGAG11100.010.40429118
GTATCAA18100.010.2360721
CTTAGAC2053.6379788E-1210.1579583
GGCGAGG5800.010.11904119
AGGACGT10700.09.7307545
TATGGCG12250.09.65938716
GTTCTAA2950.09.6366491
GGACGTG10900.09.5522096
GTAGGAC11400.09.54843
TGTACCG1201.7760598E-59.465375
TAGGACG11750.09.4250934
GACGTGA6400.09.3174747
TTAGGAC8050.09.2889973
ATGGCGA12800.09.24433517
TAGGACA4850.09.1726274
Produced by FastQC (version 0.11.5)