##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511994_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2283455 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.50925899568855 31.0 30.0 34.0 25.0 34.0 2 26.5724724157034 31.0 25.0 34.0 10.0 34.0 3 28.535536719576257 31.0 28.0 34.0 16.0 34.0 4 33.04594528904664 35.0 33.0 37.0 27.0 37.0 5 33.49726007300341 35.0 35.0 37.0 28.0 37.0 6 33.77841866820235 37.0 35.0 37.0 29.0 37.0 7 33.85151010201646 37.0 35.0 37.0 29.0 37.0 8 33.88702908531151 37.0 35.0 37.0 30.0 37.0 9 35.52212108405902 39.0 37.0 39.0 30.0 39.0 10 35.43455728271413 39.0 37.0 39.0 30.0 39.0 11 35.44493453998437 39.0 37.0 39.0 30.0 39.0 12 35.337157509125426 39.0 37.0 39.0 30.0 39.0 13 35.33865961886703 39.0 37.0 39.0 30.0 39.0 14 36.55597460865224 40.0 37.0 41.0 30.0 41.0 15 36.49267579172788 40.0 37.0 41.0 29.0 41.0 16 36.4397708735228 40.0 37.0 41.0 29.0 41.0 17 36.39963870538285 40.0 37.0 41.0 29.0 41.0 18 36.34556800987976 40.0 37.0 41.0 29.0 41.0 19 36.36884852121018 40.0 37.0 41.0 29.0 41.0 20 36.26940360112199 40.0 37.0 41.0 28.0 41.0 21 36.21760533927754 40.0 37.0 41.0 28.0 41.0 22 36.11129363179918 40.0 37.0 41.0 28.0 41.0 23 36.022249179423284 40.0 37.0 41.0 27.0 41.0 24 35.92633531206001 40.0 37.0 41.0 27.0 41.0 25 35.82838943618333 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.395887436672318 1101 2 4.217375503684714 1101 3 2.34596840844042 1101 4 1.4514893641472 1101 5 1.095706587082958 1101 6 0.801647528343949 1101 7 0.47312803600589604 1101 8 0.4014269895984839 1101 9 0.5137059575756169 1101 10 0.6684891745271813 1101 11 0.787598626345499 1101 12 0.745398516048013 1101 13 0.8296787325936918 1101 14 0.5641813764660455 1101 15 0.7207150590643252 1101 16 0.7371657756963756 1101 17 0.7776242763191661 1101 18 0.9495882557355628 1101 19 0.7503756502512715 1101 20 0.9408377615962493 1101 21 0.8699527585769005 1101 22 0.9154444874778633 1101 23 0.8463814809444941 1101 24 0.8252403133032047 1101 25 0.6216670558896595 1102 1 1.6928751280699466 1102 2 4.293339764209755 1102 3 2.6778310149259177 1102 4 1.8043923026756943 1102 5 1.259841328413792 1102 6 1.057258229384999 1102 7 0.8630429126111068 1102 8 0.8759127227355066 1102 9 0.9243442692787056 1102 10 0.8800983284610879 1102 11 1.0482179242318779 1102 12 1.0232505072984068 1102 13 1.0875645911893983 1102 14 1.0955639247075126 1102 15 1.1067166646755098 1102 16 0.9566258483064018 1102 17 0.8406380150205734 1102 18 0.9168129860481358 1102 19 1.0662536498027393 1102 20 1.0278446618240906 1102 21 0.9836732587215309 1102 22 1.0043966474149357 1102 23 1.1735458870796194 1102 24 1.1225546523211847 1102 25 0.9437789871409024 1103 1 1.2958829611979183 1103 2 4.074981892104745 1103 3 2.3996229529649966 1103 4 1.5592204264969567 1103 5 1.1671671261858165 1103 6 1.006528352854204 1103 7 0.8336466795535458 1103 8 0.784038492846399 1103 9 0.774952185390049 1103 10 0.7449910158652173 1103 11 0.801673993334397 1103 12 0.7919626304155614 1103 13 0.947399693158367 1103 14 0.9553314455162223 1103 15 1.0002276159667858 1103 16 0.8239420275505012 1103 17 0.9123872040466523 1103 18 0.9621835194049382 1103 19 0.979353707639639 1103 20 1.1542827260482014 1103 21 1.131246937902766 1103 22 1.0786746291585914 1103 23 1.3508505618099207 1103 24 1.4669498166534183 1103 25 1.4216485146385764 1104 1 1.438856223630772 1104 2 3.991285385539303 1104 3 2.1929815232432652 1104 4 1.5772984797664265 1104 5 1.2349988229520719 1104 6 1.0050560524927334 1104 7 0.8349677848201864 1104 8 0.8711371069058913 1104 9 0.7945739972214412 1104 10 0.7855816701783311 1104 11 0.7287489390435908 1104 12 0.8230046826950783 1104 13 0.7325564741930108 1104 14 0.7693497144151777 1104 15 0.7481901858128168 1104 16 0.8272467514604145 1104 17 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13 -0.9640089994364303 2315 14 -1.1055965038536044 2315 15 -1.3432029027761132 2315 16 -1.2485257876710065 2315 17 -1.1167440773943653 2315 18 -1.1616575581089847 2315 19 -1.0348887329943182 2315 20 -1.1114939007706468 2315 21 -1.058749410882868 2315 22 -1.314137211594364 2315 23 -1.2964840517022793 2315 24 -1.1757835906057323 2315 25 -1.0078174614527313 2316 1 -0.7246626384504857 2316 2 -0.3397867081673738 2316 3 -0.2820413934102177 2316 4 -0.6364044863276348 2316 5 -0.8373932992038746 2316 6 -0.8645701362807756 2316 7 -1.0878362366975338 2316 8 -1.1848041927089312 2316 9 -1.2398284878541403 2316 10 -1.4664328776978834 2316 11 -1.6175168675244365 2316 12 -1.3616085349848106 2316 13 -1.556177294901559 2316 14 -1.7525069069277137 2316 15 -1.342886250252839 2316 16 -1.5610829802788402 2316 17 -1.7229675900088424 2316 18 -1.6583152686923555 2316 19 -1.514217681456131 2316 20 -1.5978566901331348 2316 21 -1.599334821448359 2316 22 -1.7644463097830894 2316 23 -1.8011876322050426 2316 24 -1.799482419432067 2316 25 -1.6012837174993493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75472.0 3 4.0 4 621.0 5 2987.0 6 3609.0 7 3370.0 8 3037.0 9 2954.0 10 2812.0 11 2890.0 12 2753.0 13 2754.0 14 2764.0 15 2933.0 16 2960.0 17 3411.0 18 3927.0 19 4752.0 20 6203.0 21 7890.0 22 9753.0 23 11486.0 24 13392.0 25 15367.0 26 17404.0 27 19875.0 28 22760.0 29 27818.0 30 35236.0 31 45133.0 32 58513.0 33 77701.0 34 108745.0 35 158351.0 36 257943.0 37 359903.0 38 905972.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90388906284556 18.640919133506024 12.951120122796379 25.504071680852043 2 17.32382171752424 21.468259313532737 38.288108711437296 22.919810257505727 3 18.346015139339293 24.441208607132616 29.7494367088469 27.463339544681197 4 12.428885176191342 16.914106036685634 38.28702558184856 32.36998320527446 5 13.592341429982199 37.27653927929387 35.69730955941764 13.433809731306287 6 31.796362789161854 37.12312011677052 18.15373471667689 12.926782377390733 7 28.053716845744713 31.450017626797987 22.491794232862045 18.004471294595252 8 25.315103647761834 36.25050635988009 20.723202340313254 17.711187652044817 9 26.26800177800745 15.769699862708045 20.308217153392558 37.65408120589195 10 14.761322102394006 28.97413304581568 34.349498610438395 21.915046241351916 11 34.16029656813907 22.769268498831813 24.37586902303746 18.694565909991656 12 22.871569617093396 24.762388573455574 31.66329093413271 20.702750875318323 13 28.565003470617988 21.864017464762828 25.864096292679296 23.70688277193989 14 21.32373968394385 21.55435513290168 27.684495643662782 29.437409539491693 15 23.13669417614974 29.858219233573685 23.556452831345485 23.44863375893109 16 22.768699186101763 28.182644282457943 26.66179101405546 22.38686551738484 17 22.145257953408322 28.11818056410133 27.365154995390757 22.371406487099595 18 22.001240231860294 27.67122819740708 28.569389045669613 21.75814252506302 19 23.50601570174136 27.101072460378877 27.3801249955659 22.012786842313858 20 23.556566309486897 27.087723631496985 27.751151331059575 21.604558727956544 21 23.398118501720905 26.700878911339398 27.03380451810091 22.867198068838785 22 23.318106194077927 27.343072220959495 27.207135953347233 22.13168563161534 23 22.889354950454074 27.136505189326442 27.250237252002137 22.72390260821735 24 23.008191107148303 26.887317972963743 27.748032365033293 22.356458554854665 25 23.09816286759071 27.09733079331713 27.293568941732904 22.51093739735926 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 177.0 1 177.0 2 321.0 3 465.0 4 465.0 5 465.0 6 1129.0 7 1793.0 8 1793.0 9 1793.0 10 2889.0 11 3985.0 12 3985.0 13 3985.0 14 7062.0 15 10139.0 16 10139.0 17 10139.0 18 18290.0 19 26441.0 20 26441.0 21 26441.0 22 40967.5 23 55494.0 24 55494.0 25 55494.0 26 77813.0 27 100132.0 28 100132.0 29 100132.0 30 127928.0 31 155724.0 32 155724.0 33 155724.0 34 181428.0 35 207132.0 36 207132.0 37 207132.0 38 229036.0 39 250940.0 40 250940.0 41 250940.0 42 267159.5 43 283379.0 44 283379.0 45 283379.0 46 290629.5 47 297880.0 48 297880.0 49 297880.0 50 292153.5 51 286427.0 52 286427.0 53 286427.0 54 259675.0 55 232923.0 56 232923.0 57 232923.0 58 200603.5 59 168284.0 60 168284.0 61 168284.0 62 136567.0 63 104850.0 64 104850.0 65 104850.0 66 80708.5 67 56567.0 68 56567.0 69 56567.0 70 41179.5 71 25792.0 72 25792.0 73 25792.0 74 17806.0 75 9820.0 76 9820.0 77 9820.0 78 6713.0 79 3606.0 80 3606.0 81 3606.0 82 2308.5 83 1011.0 84 1011.0 85 1011.0 86 665.0 87 319.0 88 319.0 89 319.0 90 212.5 91 106.0 92 106.0 93 106.0 94 80.0 95 54.0 96 54.0 97 54.0 98 34.5 99 15.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.4409261404319333 3 0.0 4 0.0 5 0.0 6 8.758657385409391E-5 7 0.0 8 0.0 9 0.0 10 4.3793286927046954E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.817261561975165E-4 19 6.131060169786574E-4 20 4.817261561975165E-4 21 0.002715183789476911 22 0.0014013851816655025 23 7.882791646868451E-4 24 1.3137986078114085E-4 25 2.1896643463523475E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2283455.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.07060431289683 #Duplication Level Percentage of deduplicated Percentage of total 1 81.34721132105999 59.44089890398776 2 12.585370212735752 18.39241213892265 3 3.3741545368358663 7.3965453315509775 4 1.2242932254574266 3.578393833614393 5 0.5434555356156036 1.985531220231059 6 0.2870903692528098 1.2586720064229304 7 0.16176721254490253 0.8274299585068198 8 0.1169128108406291 0.6834311792035331 9 0.07401845678615927 0.4867716030905428 >10 0.26436385559983555 3.1917939009080345 >50 0.013118954334291267 0.6581743372128116 >100 0.006851524397072267 0.9768112781263872 >500 7.86773870268359E-4 0.4170239211929704 >1k 6.05210669436828E-4 0.706110387029162 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3032 0.13278124596280635 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.3793286927046954E-5 0.0 0.0 0.0 3 0.0 4.3793286927046954E-5 0.0 0.0 0.0 4 0.0 4.3793286927046954E-5 0.0 4.3793286927046954E-5 0.0 5 0.0 4.3793286927046954E-5 0.0 4.3793286927046954E-5 0.0 6 0.0 4.3793286927046954E-5 0.0 4.3793286927046954E-5 0.0 7 0.0 4.3793286927046954E-5 0.0 4.3793286927046954E-5 0.0 8 0.0 4.3793286927046954E-5 0.0 4.3793286927046954E-5 0.0 9 0.0 4.3793286927046954E-5 0.0 4.3793286927046954E-5 0.0 10 0.0 4.3793286927046954E-5 0.0 4.3793286927046954E-5 0.0 11 0.0 4.3793286927046954E-5 0.0 4.3793286927046954E-5 0.0 12 0.0 4.3793286927046954E-5 0.0 4.3793286927046954E-5 0.0 13 0.0 4.3793286927046954E-5 0.0 4.3793286927046954E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 80 2.0793523E-5 12.253498 1 GGTATCA 920 0.0 11.40108 1 TAGGACC 410 0.0 11.0814085 4 GCTAACC 60 0.0060389987 11.0429325 3 GTCTTAC 170 7.8216544E-11 10.956069 1 TGGCGAG 1110 0.0 10.404291 18 GTATCAA 1810 0.0 10.236072 1 CTTAGAC 205 3.6379788E-12 10.157958 3 GGCGAGG 580 0.0 10.119041 19 AGGACGT 1070 0.0 9.730754 5 TATGGCG 1225 0.0 9.659387 16 GTTCTAA 295 0.0 9.636649 1 GGACGTG 1090 0.0 9.552209 6 GTAGGAC 1140 0.0 9.5484 3 TGTACCG 120 1.7760598E-5 9.46537 5 TAGGACG 1175 0.0 9.425093 4 GACGTGA 640 0.0 9.317474 7 TTAGGAC 805 0.0 9.288997 3 ATGGCGA 1280 0.0 9.244335 17 TAGGACA 485 0.0 9.172627 4 >>END_MODULE