FastQCFastQC Report
Thu 26 May 2016
SRR1511994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283455
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36670.16058998316148118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTT604.1098037E-412.66063813
GGTATCA11150.012.5006041
TAGGACC3850.010.6137064
GGCGAGG4700.010.50724619
TCTACAC4100.010.1983083
GTATCAA22400.010.1433621
GACGTGA5350.010.1166787
TTAGACT1909.822543E-1110.0031254
GTGGTAT4900.09.9363551
GGACGTG10150.09.9201536
TTAGAAC2900.09.8306583
ATAGGAC3000.09.8197353
AGGACGT10100.09.6911475
ACGTGAA5950.09.5752738
TATTGCG1002.747472E-49.5029695
TGGCGAG10500.09.49547918
TATGGCG11050.09.36658116
TAGGACG10950.09.2860074
ATTAGAC1451.6995364E-69.1752813
ATTGGAC1356.822027E-69.1510083
Produced by FastQC (version 0.11.5)