FastQCFastQC Report
Thu 26 May 2016
SRR1511993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362426
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30860.13062843026617552No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAT250.00616327218.92983810
CGGTAGG801.3976223E-613.4860821
GGCGTGC1501.8189894E-1212.6198938
GCGTGCG1104.0188752E-812.0462619
TCGAACT1603.6379788E-1211.83164919
GGTATCA10050.011.8087091
GTCAACG853.8458846E-511.5388952
GTATCAA21750.010.9128751
CGTGCGC1408.85484E-910.81705110
GCGCCAC1855.456968E-1210.74396213
TAGGACA4650.010.5844254
GTAGGAC13300.010.5323923
TAGGACC5800.010.2808614
GTACTAA1451.015469E-710.1462691
GTCCTAC11200.010.0707761
CAAGTCG1606.5629138E-910.0564776
CTAGGAC3400.010.021683
AGGACGT14100.010.0019365
AAAGGCG1804.2200554E-109.9907485
TAGGACG14550.09.9528014
Produced by FastQC (version 0.11.5)