##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511993_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2362426 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.35494445117011 31.0 30.0 34.0 24.0 34.0 2 26.408900003640326 31.0 25.0 34.0 10.0 34.0 3 28.376019820303366 31.0 28.0 34.0 16.0 34.0 4 32.87812316660924 35.0 33.0 37.0 26.0 37.0 5 33.329701332443854 35.0 35.0 37.0 28.0 37.0 6 33.61180963975168 37.0 35.0 37.0 28.0 37.0 7 33.68351474289565 37.0 35.0 37.0 28.0 37.0 8 33.72322519308541 37.0 35.0 37.0 29.0 37.0 9 35.336113808432515 39.0 35.0 39.0 29.0 39.0 10 35.24913669253556 39.0 35.0 39.0 29.0 39.0 11 35.2532468741878 39.0 35.0 39.0 29.0 39.0 12 35.15571620021114 39.0 35.0 39.0 29.0 39.0 13 35.151638612172405 39.0 35.0 39.0 29.0 39.0 14 36.34591771340139 40.0 37.0 41.0 29.0 41.0 15 36.28403937308512 40.0 37.0 41.0 28.0 41.0 16 36.23039790452696 40.0 37.0 41.0 28.0 41.0 17 36.18856971604613 40.0 37.0 41.0 28.0 41.0 18 36.13247695377548 40.0 36.0 41.0 27.0 41.0 19 36.16015443446694 40.0 37.0 41.0 27.0 41.0 20 36.059610332768095 40.0 37.0 41.0 27.0 41.0 21 36.00199244336119 40.0 37.0 41.0 27.0 41.0 22 35.89813268225121 40.0 36.0 41.0 27.0 41.0 23 35.800996941279855 40.0 36.0 41.0 27.0 41.0 24 35.70096206188045 40.0 36.0 41.0 27.0 41.0 25 35.59957899210388 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2457466998290379 1101 2 4.095663965379895 1101 3 2.3066807667220175 1101 4 1.3727436350921636 1101 5 1.0120522154797271 1101 6 0.6207378354620658 1101 7 0.33013213374495365 1101 8 0.23349601229717365 1101 9 0.3659505956897391 1101 10 0.44462610730506924 1101 11 0.43224698431168207 1101 12 0.32358927813080385 1101 13 0.417589797972731 1101 14 0.3557241228722674 1101 15 0.5711910520776229 1101 16 0.4770351620098907 1101 17 0.4801203258461939 1101 18 0.5957890736932541 1101 19 0.5994547072720593 1101 20 0.5990871784767648 1101 21 0.5725863840256551 1101 22 0.5808805974562006 1101 23 0.638274734724682 1101 24 0.5884970028471983 1101 25 0.46263664986494746 1102 1 1.6426923840985843 1102 2 4.330588391068677 1102 3 2.6667623985577364 1102 4 1.8113983030862784 1102 5 1.4191293978754231 1102 6 1.1596833865226657 1102 7 1.072308611221544 1102 8 0.9565361103668195 1102 9 0.9787903274783076 1102 10 0.9290273831507605 1102 11 0.9679676599509435 1102 12 1.0256561498928178 1102 13 1.0954888229790143 1102 14 1.1151714015136065 1102 15 1.1313723294295315 1102 16 1.1511462208338301 1102 17 1.0835318243902563 1102 18 1.193318047727523 1102 19 1.3064855712626766 1102 20 1.2837082490849099 1102 21 1.1747831127447625 1102 22 1.0939022073429143 1102 23 1.253710517346839 1102 24 1.1828846835444793 1102 25 1.2448626584329787 1103 1 1.3373786074996303 1103 2 4.029471030542897 1103 3 2.3442770483434217 1103 4 1.4613597121540138 1103 5 1.031610514431172 1103 6 0.8697507611963147 1103 7 0.5844252649960282 1103 8 0.55887763605066 1103 9 0.6973960796889926 1103 10 0.6315097025963539 1103 11 0.6038199416531924 1103 12 0.6226773494351221 1103 13 0.6008966903922968 1103 14 0.645455330605607 1103 15 0.7828583264646838 1103 16 0.5931200560533298 1103 17 0.7198190299658407 1103 18 0.8619627761376876 1103 19 0.7474945261568777 1103 20 0.8653853258143016 1103 21 0.881883287784504 1103 22 0.8528910264603837 1103 23 0.972428853197961 1103 24 1.0580223630629462 1103 25 0.912802561885016 1104 1 1.529396345128884 1104 2 3.9100506638730472 1104 3 2.25503665869498 1104 4 1.7317013623329345 1104 5 1.245652145845341 1104 6 1.0281944060355102 1104 7 0.9847062424750277 1104 8 0.945338126370352 1104 9 0.9683056105372643 1104 10 1.0307664373318843 1104 11 0.8434120881104405 1104 12 0.91894151764631 1104 13 0.7628319783324855 1104 14 0.9813748528615918 1104 15 1.0248808375836234 1104 16 1.050001452941018 1104 17 0.9210788197347739 1104 18 0.8579361729858661 1104 19 1.0747855543007176 1104 20 0.9678577465661391 1104 21 0.9702903742768427 1104 22 0.9036188190341505 1104 23 1.0818273605771225 1104 24 0.9694268060296238 1104 25 1.0622506477156577 1105 1 1.4495496294489314 1105 2 4.311799500027519 1105 3 2.662514240008832 1105 4 1.5901646202305457 1105 5 1.15808296363614 1105 6 1.0119471690521706 1105 7 0.8403167778983445 1105 8 0.7405209242907276 1105 9 0.7833877593137686 1105 10 0.6207256386627904 1105 11 0.7115915339071606 1105 12 0.6453760727909241 1105 13 0.7030554817211154 1105 14 0.722155736125984 1105 15 0.6589386003595195 1105 16 0.7836936354096267 1105 17 0.8294427799007309 1105 18 0.7931815863393723 1105 19 0.8464143633026779 1105 20 0.7381644627742361 1105 21 0.7599316053978455 1105 22 0.8540058743690437 1105 23 0.8097888562021183 1105 24 0.8756809321086152 1105 25 1.0315013595370957 1106 1 0.48000667785665385 1106 2 2.2020926965734446 1106 3 1.140498172270373 1106 4 0.3666884837524407 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0.7371032346591662 1107 17 0.7016732614109955 1107 18 0.6768745573216037 1107 19 0.6543414957690814 1107 20 0.964825685663989 1107 21 0.9477310598388229 1107 22 0.9440505974795812 1107 23 0.9139705230899793 1107 24 0.9857259300599637 1107 25 0.9900060012730449 1108 1 1.1707234681772647 1108 2 3.434724905891958 1108 3 2.085698542805975 1108 4 1.3148399614904704 1108 5 0.9539738505598834 1108 6 0.7873547450464997 1108 7 0.5724555588568521 1108 8 0.5951844478465915 1108 9 0.6808183176652562 1108 10 0.6084391638947366 1108 11 0.5647941175201225 1108 12 0.5587524616105881 1108 13 0.603226654859256 1108 14 0.5504239041832051 1108 15 0.7131755301615073 1108 16 0.701015998716322 1108 17 0.7628823064405523 1108 18 0.7581172540786696 1108 19 0.7702840858411477 1108 20 0.7094506166924219 1108 21 0.6539271723842575 1108 22 0.7018453522404258 1108 23 0.7667997627918837 1108 24 0.6596416667537142 1108 25 0.6344690700506987 1109 1 0.9461992690492451 1109 2 3.12129372606859 1109 3 1.8453271270993277 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4 0.3600123933631707 2314 5 -0.0011846871444305407 2314 6 -0.16518552838024192 2314 7 -0.32159813030700946 2314 8 -0.42659728263276264 2314 9 -0.6217304757704056 2314 10 -0.5314069516365834 2314 11 -0.4421201974056217 2314 12 -0.41542208060278085 2314 13 -0.5847214648403138 2314 14 -0.5617452900861082 2314 15 -0.5154392474662615 2314 16 -0.5636941243650426 2314 17 -0.46058441964771646 2314 18 -0.5063864226328647 2314 19 -0.4383250554730367 2314 20 -0.3909707960561306 2314 21 -0.4456061932814137 2314 22 -0.4393115338929334 2314 23 -0.7135026319860032 2314 24 -0.5457495327324651 2314 25 -0.6038486869586421 2315 1 -3.0613400434097784 2315 2 -11.038407792005282 2315 3 -4.961665550580058 2315 4 -2.4937298602882088 2315 5 -1.3970382553403304 2315 6 -1.204205011827625 2315 7 -0.9436485521904174 2315 8 -0.9744574144710256 2315 9 -0.9818391941830598 2315 10 -0.9864665098150027 2315 11 -1.1072366759429713 2315 12 -1.0658941089350193 2315 13 -1.158038351532909 2315 14 -1.3426445541320788 2315 15 -1.4105655585296262 2315 16 -1.3226041323144386 2315 17 -1.2519137321231923 2315 18 -1.3525700417020232 2315 19 -1.2782762570105888 2315 20 -1.2794098394261013 2315 21 -1.2727935589646577 2315 22 -1.5356463304454167 2315 23 -1.48469527139153 2315 24 -1.3608426997498029 2315 25 -1.4637363891615252 2316 1 -0.8551881718040697 2316 2 -0.33961258956804485 2316 3 -0.3685232217658587 2316 4 -0.6700240824482506 2316 5 -1.087102147673285 2316 6 -1.0742569211800657 2316 7 -1.2979218004173276 2316 8 -1.387415727148415 2316 9 -1.4908585891968613 2316 10 -1.5541155488392135 2316 11 -1.584483374963007 2316 12 -1.6447775019636026 2316 13 -1.6868198644455887 2316 14 -1.9160843532847949 2316 15 -1.796060339715197 2316 16 -1.9100603465058725 2316 17 -2.0485610433138035 2316 18 -2.0408279260762896 2316 19 -1.8925561894832583 2316 20 -1.9191099562708587 2316 21 -1.9631976336736585 2316 22 -2.135805315016789 2316 23 -1.9982496804648946 2316 24 -2.0589345582280743 2316 25 -1.9931676278838282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84346.0 3 0.0 4 783.0 5 3460.0 6 3808.0 7 3626.0 8 3356.0 9 3315.0 10 3130.0 11 3063.0 12 3095.0 13 3124.0 14 3069.0 15 3232.0 16 3340.0 17 3777.0 18 4345.0 19 5247.0 20 6919.0 21 8639.0 22 10636.0 23 12805.0 24 14629.0 25 16854.0 26 18789.0 27 21674.0 28 24900.0 29 30240.0 30 37686.0 31 47505.0 32 61525.0 33 81662.0 34 113080.0 35 163883.0 36 264567.0 37 367474.0 38 920843.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.97781179177676 18.682659266364322 13.060091617684533 25.279437324174385 2 17.31315826872365 21.35185565328154 38.37718721105479 22.957798866940017 3 18.446249744965556 24.44343230221814 29.63728810976513 27.473029843051165 4 12.4529191602192 16.92006437450316 38.19827584017447 32.428740625103174 5 13.636067330786233 37.32722210134836 35.61034292714354 13.426367640721868 6 31.812044059792772 36.99163487025625 18.244169341177248 12.952151728773725 7 28.221878695882964 31.464054323817976 22.339705032030633 17.974361948268434 8 25.377874589828075 36.28095549317142 20.637870255297102 17.703299661703404 9 26.291701835316744 15.781912322333058 20.32283762539017 37.60354821696003 10 15.01145222510956 28.890888718912745 34.20953825796651 21.888120798011197 11 34.06798773802862 22.786660830857773 24.453295045008815 18.69205638610479 12 23.18036628448891 24.636030927529582 31.43810642111118 20.745496366870327 13 28.597678826765367 21.911797448893637 25.85062981866945 23.639893905671546 14 21.326466945419668 21.697272210854436 27.562133163112833 29.414127680613067 15 23.211986322534546 30.11218129160448 23.380245561130803 23.295586824730172 16 22.705981055067966 28.09747268274223 26.896800153740262 22.299746108449533 17 22.116629261614968 28.260356091577048 27.426636855503624 22.19637779130436 18 21.958420673003662 27.576082870814044 28.811889821640825 21.653606634541468 19 23.553713935708956 27.035789254461434 27.386913295854704 22.023583513974906 20 23.699033659171942 27.085861640853416 27.591064574967078 21.624040125007564 21 23.45378466071077 26.67153466970599 26.980166062399334 22.8945146071839 22 23.48552031659611 27.372407221703206 27.09552531579395 22.046547145906732 23 22.855545212996745 27.22706121341496 27.292756921224836 22.62463665236346 24 23.184641550677142 26.69632826594357 27.81640567789213 22.302624505487156 25 23.103828365682958 26.980280373752656 27.51278562896151 22.403105631602873 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 165.0 1 165.0 2 320.5 3 476.0 4 476.0 5 476.0 6 1120.5 7 1765.0 8 1765.0 9 1765.0 10 2795.5 11 3826.0 12 3826.0 13 3826.0 14 6799.0 15 9772.0 16 9772.0 17 9772.0 18 18056.0 19 26340.0 20 26340.0 21 26340.0 22 41272.0 23 56204.0 24 56204.0 25 56204.0 26 80084.0 27 103964.0 28 103964.0 29 103964.0 30 133746.0 31 163528.0 32 163528.0 33 163528.0 34 189116.5 35 214705.0 36 214705.0 37 214705.0 38 236898.0 39 259091.0 40 259091.0 41 259091.0 42 276162.5 43 293234.0 44 293234.0 45 293234.0 46 301060.0 47 308886.0 48 308886.0 49 308886.0 50 303220.0 51 297554.0 52 297554.0 53 297554.0 54 268960.5 55 240367.0 56 240367.0 57 240367.0 58 206946.0 59 173525.0 60 173525.0 61 173525.0 62 141280.5 63 109036.0 64 109036.0 65 109036.0 66 83473.5 67 57911.0 68 57911.0 69 57911.0 70 42207.5 71 26504.0 72 26504.0 73 26504.0 74 18356.5 75 10209.0 76 10209.0 77 10209.0 78 6908.5 79 3608.0 80 3608.0 81 3608.0 82 2427.5 83 1247.0 84 1247.0 85 1247.0 86 802.0 87 357.0 88 357.0 89 357.0 90 225.5 91 94.0 92 94.0 93 94.0 94 69.0 95 44.0 96 44.0 97 44.0 98 29.0 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.495643884718506 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 8.465873640063223E-5 9 0.0 10 1.2698810460094834E-4 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 8.465873640063223E-5 19 5.502817866041094E-4 20 3.8096431380284506E-4 21 0.0025820914602192827 22 0.001989480305414857 23 8.042579958060062E-4 24 0.0 25 3.3863494560252893E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2362426.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.003781199955 #Duplication Level Percentage of deduplicated Percentage of total 1 81.12845265499921 58.41555354061471 2 12.57642603888241 18.11100457562212 3 3.438644962189815 7.42786318445529 4 1.263482041575912 3.6390193788681766 5 0.5892603465238331 2.121448653045587 6 0.2955196242865877 1.276711822045462 7 0.1839769854252502 0.9272927023070918 8 0.11607289701260669 0.6686149983792514 9 0.0742909995154505 0.48143095868128144 >10 0.30974385876286215 3.679417903186963 >50 0.014265770334082929 0.713694332747945 >100 0.00814335975150239 1.188257491821127 >500 0.001245850881043635 0.6626708468956456 >1k 4.74609859440687E-4 0.6870196113294026 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3086 0.13062843026617552 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.232936820031612E-5 2 0.0 0.0 0.0 4.232936820031612E-5 4.232936820031612E-5 3 0.0 0.0 0.0 4.232936820031612E-5 4.232936820031612E-5 4 0.0 0.0 0.0 4.232936820031612E-5 4.232936820031612E-5 5 0.0 0.0 0.0 4.232936820031612E-5 4.232936820031612E-5 6 4.232936820031612E-5 0.0 0.0 4.232936820031612E-5 4.232936820031612E-5 7 4.232936820031612E-5 0.0 0.0 4.232936820031612E-5 4.232936820031612E-5 8 4.232936820031612E-5 0.0 0.0 4.232936820031612E-5 4.232936820031612E-5 9 4.232936820031612E-5 0.0 0.0 1.2698810460094834E-4 4.232936820031612E-5 10 4.232936820031612E-5 0.0 0.0 1.2698810460094834E-4 8.465873640063223E-5 11 4.232936820031612E-5 0.0 0.0 2.539762092018967E-4 8.465873640063223E-5 12 4.232936820031612E-5 0.0 0.0 3.3863494560252893E-4 8.465873640063223E-5 13 4.232936820031612E-5 0.0 0.0 3.8096431380284506E-4 8.465873640063223E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGTAT 25 0.006163272 18.929838 10 CGGTAGG 80 1.3976223E-6 13.486082 1 GGCGTGC 150 1.8189894E-12 12.619893 8 GCGTGCG 110 4.0188752E-8 12.046261 9 TCGAACT 160 3.6379788E-12 11.831649 19 GGTATCA 1005 0.0 11.808709 1 GTCAACG 85 3.8458846E-5 11.538895 2 GTATCAA 2175 0.0 10.912875 1 CGTGCGC 140 8.85484E-9 10.817051 10 GCGCCAC 185 5.456968E-12 10.743962 13 TAGGACA 465 0.0 10.584425 4 GTAGGAC 1330 0.0 10.532392 3 TAGGACC 580 0.0 10.280861 4 GTACTAA 145 1.015469E-7 10.146269 1 GTCCTAC 1120 0.0 10.070776 1 CAAGTCG 160 6.5629138E-9 10.056477 6 CTAGGAC 340 0.0 10.02168 3 AGGACGT 1410 0.0 10.001936 5 AAAGGCG 180 4.2200554E-10 9.990748 5 TAGGACG 1455 0.0 9.952801 4 >>END_MODULE