FastQCFastQC Report
Thu 26 May 2016
SRR1511993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362426
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38120.16135955157960505No Hit
GTCCTACAGTGGACATTTCTAAATT25670.10865948817021147No Hit
CTGTAGGACGTGGAATATGGCAAGA25370.10738960712420198No Hit
GGTATCAACGCAGAGTACTTTTTTT24360.10311434093597006No Hit
TATCAACGCAGAGTACTTTTTTTTT24270.10273337662216721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.012.2171611
GAACCGC550.003067823612.0916636
CGTAACA752.0653401E-411.4055422
AATGCCG1158.877614E-710.73389118
TCTACAC3150.010.5606873
AGGACGT14350.010.531255
GTATCAA23750.010.3685121
ATGCCGG1106.0744715E-610.35989519
GGACGTG14400.010.2923096
TAGGACC4450.010.0385864
TGTAGGA15150.010.0378812
GTATTAG2002.3646862E-1110.021891
ACCGTGC1054.1306645E-59.94710358
TAGGACG15250.09.6604334
GTAGGAC14650.09.6019353
TTAGGAC8500.09.504623
CGTGCGC900.00111938169.49496310
GACGTGG8600.09.4949637
GTCCTAA7450.09.48061
GTCCTAC13050.09.361811
Produced by FastQC (version 0.11.5)