FastQCFastQC Report
Thu 26 May 2016
SRR1511992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009258
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26700.1328848759094153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8300.012.4094531
TCGGAAC550.003155735312.0459823
CGGTGCC600.006040632711.04242514
AGGACGT9800.010.6236435
CGTTAGG650.008394239510.5639461
GACGTGG5850.010.5163357
GGACGTG10050.010.453556
TAGGACC3550.010.397844
TAGACTG2600.010.1927555
GTATCAA16250.09.9602911
TAGGACG10950.09.8536624
GTCCTAA6050.09.7283091
GTAGGAC10750.09.5967663
TGTAGGA10650.09.579112
GGCGAGG4400.09.46517219
TTAATAC3050.09.3095423
GGGTATA1501.7497096E-69.1554181
TAATACT3850.09.0959464
GTGGTAT3700.09.0140231
CTTATAC2107.166818E-109.0143
Produced by FastQC (version 0.11.5)