FastQCFastQC Report
Thu 26 May 2016
SRR1511992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009258
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33010.16428950388650937No Hit
GGTATCAACGCAGAGTACTTTTTTT21570.1073530626728872No Hit
TATCAACGCAGAGTACTTTTTTTTT20200.10053462521985729No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCCA500.001505643513.2931559
GGTATCA9350.012.4566081
CCGAGAC600.00586001311.0875623
GTTAGAC700.001486992210.8612843
GGCGAGG4400.010.79097219
AGGACGT9600.010.2955925
TTAGGAC6100.010.282613
CTTATAC2053.6379788E-1210.1990113
GTCCTAC8550.09.9374611
GGACGTG9750.09.8423286
TAGGACG10050.09.8345974
TAGACAG3600.09.7676145
GTATCAA18200.09.7040241
ATTAGAC1701.6545528E-89.5036243
CCTAATC1106.8088884E-59.5036243
TAGGACC3350.09.3617784
GTATAAT2159.640644E-119.3246471
GTAGGAC10400.09.3208633
CTAGGAC2357.2759576E-129.3014193
GTCCTAA6400.09.2483291
Produced by FastQC (version 0.11.5)