FastQCFastQC Report
Thu 26 May 2016
SRR1511991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886317
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41220.2185210651232004No Hit
TATCAACGCAGAGTACTTTTTTTTT23300.12352112608856305No Hit
GGTATCAACGCAGAGTACTTTTTTT23210.12304400585903642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5700.012.4558964
GGTATCA9250.012.1761291
GGCGAGG5450.011.81170419
GTAGGAC11900.010.8983863
GCGCCAC700.001535945610.81912313
TGTAGGA12500.010.8124042
TGGCGAG11050.010.62330218
GTGGTAT3050.010.5966181
AGGACGT9600.010.5511825
TTCAACG650.00849314810.5472162
GGACGTG9650.010.4965136
GACGTGA6150.010.4670057
TAGGACG10000.010.3184654
CTGTAGG12350.010.3093091
GTCCTAC10500.010.2602171
GTATCAA19400.010.1977131
TCCTACA12750.09.9090652
TTAGGAC7650.09.899593
ATGGCGA11900.09.86449517
GTATTAA2600.09.7938441
Produced by FastQC (version 0.11.5)