##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511991_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1886317 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.072426320708555 31.0 30.0 34.0 25.0 34.0 2 27.108825823019142 31.0 26.0 34.0 10.0 34.0 3 29.100881241063934 31.0 28.0 34.0 16.0 34.0 4 33.65552608601842 35.0 33.0 37.0 28.0 37.0 5 34.125204300231616 37.0 35.0 37.0 30.0 37.0 6 34.425896601684656 37.0 35.0 37.0 32.0 37.0 7 34.52011035260775 37.0 35.0 37.0 32.0 37.0 8 34.570772038846066 37.0 35.0 37.0 32.0 37.0 9 36.254081896097 39.0 37.0 39.0 32.0 39.0 10 36.18569307279741 39.0 37.0 39.0 32.0 39.0 11 36.20043502762261 39.0 37.0 39.0 32.0 39.0 12 36.11456345884599 39.0 37.0 39.0 32.0 39.0 13 36.12059531881439 39.0 37.0 39.0 32.0 39.0 14 37.3900150398899 40.0 38.0 41.0 32.0 41.0 15 37.33849135643691 40.0 38.0 41.0 32.0 41.0 16 37.29351217213225 40.0 38.0 41.0 32.0 41.0 17 37.27107638853915 40.0 38.0 41.0 32.0 41.0 18 37.2232588690024 40.0 38.0 41.0 32.0 41.0 19 37.26209115434998 40.0 38.0 41.0 32.0 41.0 20 37.176071148168624 40.0 38.0 41.0 32.0 41.0 21 37.134789115509214 40.0 38.0 41.0 32.0 41.0 22 37.04658230827586 40.0 38.0 41.0 31.0 41.0 23 36.966606885269016 40.0 38.0 41.0 31.0 41.0 24 36.878154626184255 40.0 38.0 41.0 31.0 41.0 25 36.78992873414172 40.0 37.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9016031101670627 1101 2 3.8970225274688737 1101 3 1.907132624670357 1101 4 0.9610402081185043 1101 5 0.5557216431080647 1101 6 0.28803640544674636 1101 7 0.0034037953179222313 1101 8 -0.1349869853484691 1101 9 -0.1713022017689383 1101 10 0.12878495083449337 1101 11 0.004286615148672013 1101 12 -0.09040924795112204 1101 13 -0.08320748826230329 1101 14 -0.2719470919860356 1101 15 -0.1584435357676952 1101 16 -0.15065020425966225 1101 17 -0.15020894629277848 1101 18 -0.002856789690284245 1101 19 -0.01871665627094643 1101 20 0.053166991842971356 1101 21 -0.11887942050302769 1101 22 0.002700174649575615 1101 23 0.1737195302074923 1101 24 -0.07877776475445586 1101 25 -0.23048889930377925 1102 1 1.5920388770035103 1102 2 4.282421508793149 1102 3 2.6241671464586993 1102 4 1.6690198346040788 1102 5 1.1108999113593327 1102 6 1.0441201632282926 1102 7 0.7277954195397669 1102 8 0.7942716450986111 1102 9 0.7788958740658103 1102 10 0.6392546735283204 1102 11 0.7711230611022657 1102 12 0.7567893938145858 1102 13 0.7990788728016511 1102 14 0.971969150232411 1102 15 0.9315394863036133 1102 16 0.8086027668778613 1102 17 0.8582800180535699 1102 18 0.935456736059578 1102 19 0.7826415780244673 1102 20 0.9156457694321105 1102 21 1.0050594589536814 1102 22 0.9087556358833027 1102 23 0.9003748782550289 1102 24 0.982908709495689 1102 25 1.0796412648162033 1103 1 1.092320907058653 1103 2 3.9811030299781436 1103 3 2.268653061325846 1103 4 1.353353836764903 1103 5 0.9755030347369598 1103 6 0.8361896464538248 1103 7 0.4579782479784953 1103 8 0.5174846362541885 1103 9 0.4977759855682393 1103 10 0.4893372246141183 1103 11 0.6276029705533119 1103 12 0.3944457848588456 1103 13 0.42657282763006776 1103 14 0.48705237949992863 1103 15 0.49696624969302405 1103 16 0.5248215997504957 1103 17 0.6387642865761194 1103 18 0.5360694381694202 1103 19 0.46735910364336775 1103 20 0.687110688728481 1103 21 0.7157181371073449 1103 22 0.6450832584588042 1103 23 0.7937983863721669 1103 24 0.8553506781870794 1103 25 0.8170521073049883 1104 1 1.349476145419203 1104 2 3.922256871029841 1104 3 2.1118352111474223 1104 4 1.3601732345955355 1104 5 0.9379684889286679 1104 6 0.8644320883837011 1104 7 0.6255208857969237 1104 8 0.5774937452929194 1104 9 0.6029358580416968 1104 10 0.5968053098145774 1104 11 0.5276564751011961 1104 12 0.5851660621981196 1104 13 0.5931081649519214 1104 14 0.6318737141067601 1104 15 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-0.6562986095121559 2315 10 -0.7399303056205326 2315 11 -0.8102220885483433 2315 12 -0.6623543245524885 2315 13 -0.7792728633220065 2315 14 -0.7503233116475201 2315 15 -1.0920730797718363 2315 16 -0.9929128042508282 2315 17 -1.0084115335204018 2315 18 -0.8483199098562864 2315 19 -0.8881495613430559 2315 20 -0.7534965441418038 2315 21 -0.8513803135501021 2315 22 -1.1620949061661676 2315 23 -1.0371728345410318 2315 24 -0.8745401058623159 2315 25 -0.9057464781628468 2316 1 -0.3237654407713606 2316 2 0.3942810094750513 2316 3 0.1671904901166279 2316 4 -0.13433333944686154 2316 5 -0.3703089277665086 2316 6 -0.6102216670215768 2316 7 -0.5598732497537782 2316 8 -0.7057242462691491 2316 9 -0.7320096287181173 2316 10 -0.839481299137347 2316 11 -1.105105850680097 2316 12 -0.9030129412138947 2316 13 -1.062476920436069 2316 14 -1.1693033859467405 2316 15 -0.8544495476244123 2316 16 -1.114183309895246 2316 17 -1.1864794215289933 2316 18 -1.1159753873478309 2316 19 -0.9801167190932603 2316 20 -0.822558281149135 2316 21 -0.92570611176847 2316 22 -1.0414400542940143 2316 23 -1.1136822105272088 2316 24 -1.089520645223736 2316 25 -1.0268043839434569 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42717.0 3 1.0 4 370.0 5 1624.0 6 1983.0 7 1898.0 8 1837.0 9 1753.0 10 1667.0 11 1587.0 12 1613.0 13 1666.0 14 1636.0 15 1779.0 16 1895.0 17 1989.0 18 2390.0 19 2852.0 20 3721.0 21 4707.0 22 5833.0 23 7062.0 24 8174.0 25 9657.0 26 11146.0 27 12980.0 28 15250.0 29 18949.0 30 24274.0 31 32015.0 32 43302.0 33 60360.0 34 87390.0 35 132895.0 36 222485.0 37 315936.0 38 798924.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.707146783918084 19.451661624212687 13.994890572475358 24.846301019393877 2 16.64797397758303 21.51851179504111 39.6378426315065 22.195671595869364 3 18.357147817678577 24.681376460054167 29.98578711849599 26.975688603771264 4 12.055502866167247 17.441554097216958 39.280990416775126 31.221952619840675 5 13.16125550477465 37.56733359239195 36.042192272030626 13.229218630802775 6 30.61576109199095 37.93256273074076 18.719716102710258 12.731960074558026 7 27.41066321302305 31.881650857199507 22.69263331666947 18.015052613107976 8 24.311290202018007 37.23642420653581 21.044129910296093 17.40815568115009 9 25.481295031534994 16.554746630603447 21.101755431351148 36.86220290651041 10 14.402616315285288 29.574138387132177 34.6847322056685 21.33851309191403 11 33.23608916210796 23.487091512190155 24.808449481184763 18.46836984451712 12 22.540803056962325 25.303700279433418 31.97993762448199 20.175559039122266 13 27.762936982490217 22.46976515612169 26.473864149026916 23.29343371236118 14 20.45727202797833 22.533328173366407 28.39602251371323 28.613377284942032 15 22.325144713216282 30.69971802194435 24.245235556907986 22.729901707931383 16 21.676738321289584 29.160051041261887 27.64779196709779 21.51541867035074 17 21.30649302317691 29.011613636520266 28.100738104995077 21.581155235307744 18 21.03347970399304 28.339236402133906 29.78942452103502 20.83785937283804 19 22.574394632432305 27.748885114287713 28.346989755562767 21.329730497717215 20 22.616526940012786 27.71597549066904 28.893199498277582 20.774298071040587 21 22.487711053901712 27.268100483075397 28.071123203311547 22.17306525971134 22 22.263608796149033 28.139777763640605 28.391755200712517 21.20485823949785 23 21.93288310234211 27.90910840374046 28.30994793486265 21.84806055905478 24 22.1003267745171 27.444394258438145 29.05759162303665 21.39768734400811 25 22.109456260012955 27.74973943782186 28.486205313453915 21.654598988711268 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 163.0 1 163.0 2 334.0 3 505.0 4 505.0 5 505.0 6 1271.0 7 2037.0 8 2037.0 9 2037.0 10 3329.5 11 4622.0 12 4622.0 13 4622.0 14 7944.5 15 11267.0 16 11267.0 17 11267.0 18 19207.5 19 27148.0 20 27148.0 21 27148.0 22 41541.0 23 55934.0 24 55934.0 25 55934.0 26 76695.5 27 97457.0 28 97457.0 29 97457.0 30 122943.0 31 148429.0 32 148429.0 33 148429.0 34 169378.0 35 190327.0 36 190327.0 37 190327.0 38 205418.0 39 220509.0 40 220509.0 41 220509.0 42 230222.0 43 239935.0 44 239935.0 45 239935.0 46 242178.0 47 244421.0 48 244421.0 49 244421.0 50 234366.5 51 224312.0 52 224312.0 53 224312.0 54 197583.0 55 170854.0 56 170854.0 57 170854.0 58 143928.5 59 117003.0 60 117003.0 61 117003.0 62 93828.5 63 70654.0 64 70654.0 65 70654.0 66 53403.0 67 36152.0 68 36152.0 69 36152.0 70 25933.0 71 15714.0 72 15714.0 73 15714.0 74 10811.5 75 5909.0 76 5909.0 77 5909.0 78 3989.0 79 2069.0 80 2069.0 81 2069.0 82 1343.5 83 618.0 84 618.0 85 618.0 86 391.0 87 164.0 88 164.0 89 164.0 90 117.0 91 70.0 92 70.0 93 70.0 94 50.0 95 30.0 96 30.0 97 30.0 98 22.0 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.338039152486035 3 0.0 4 0.0 5 0.0 6 5.301335883629316E-5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 5.301335883629316E-4 19 0.001325333970907329 20 7.952003825443974E-4 21 0.0036579217597042277 22 0.001961494276942847 23 0.0013783473297436222 24 5.301335883629316E-5 25 5.831469471992248E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1886317.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.76374182467035 #Duplication Level Percentage of deduplicated Percentage of total 1 84.90830327435388 66.02787374597668 2 10.863352532717977 16.89549883409319 3 2.524121895234404 5.888554901850174 4 0.7845315609928105 2.440324390494021 5 0.33561102552085 1.3049184571058114 6 0.17869930551397867 0.8337795994942157 7 0.09847273825782102 0.5360326016254672 8 0.06832933406143005 0.42508357544037595 9 0.04395070543375755 0.30759941793265727 >10 0.1760435865957189 2.290729908334766 >50 0.01019331761881928 0.5664689843770532 >100 0.007153302354776569 1.1793197790371184 >500 4.812194114648274E-4 0.2772927375696042 >1k 7.562019323018685E-4 1.026523066668888 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4122 0.2185210651232004 No Hit TATCAACGCAGAGTACTTTTTTTTT 2330 0.12352112608856305 No Hit GGTATCAACGCAGAGTACTTTTTTT 2321 0.12304400585903642 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.301335883629316E-5 0.0 10 0.0 0.0 0.0 5.301335883629316E-5 0.0 11 0.0 0.0 0.0 5.301335883629316E-5 0.0 12 0.0 0.0 0.0 5.301335883629316E-5 0.0 13 0.0 0.0 0.0 5.301335883629316E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 570 0.0 12.455896 4 GGTATCA 925 0.0 12.176129 1 GGCGAGG 545 0.0 11.811704 19 GTAGGAC 1190 0.0 10.898386 3 GCGCCAC 70 0.0015359456 10.819123 13 TGTAGGA 1250 0.0 10.812404 2 TGGCGAG 1105 0.0 10.623302 18 GTGGTAT 305 0.0 10.596618 1 AGGACGT 960 0.0 10.551182 5 TTCAACG 65 0.008493148 10.547216 2 GGACGTG 965 0.0 10.496513 6 GACGTGA 615 0.0 10.467005 7 TAGGACG 1000 0.0 10.318465 4 CTGTAGG 1235 0.0 10.309309 1 GTCCTAC 1050 0.0 10.260217 1 GTATCAA 1940 0.0 10.197713 1 TCCTACA 1275 0.0 9.909065 2 TTAGGAC 765 0.0 9.89959 3 ATGGCGA 1190 0.0 9.864495 17 GTATTAA 260 0.0 9.793844 1 >>END_MODULE