FastQCFastQC Report
Thu 26 May 2016
SRR1511991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886317
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50820.2694138896060418No Hit
GGTATCAACGCAGAGTACTTTTTTT31490.16693906697548716No Hit
TATCAACGCAGAGTACTTTTTTTTT30440.16137266429767635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4250.012.1278081
GGTATCA11250.011.9631091
TGGTATC4200.011.7681142
TAGGACC5300.011.1190754
GACGTGG4900.011.0448387
TCCAACG1053.4857785E-610.85221918
GTAGGAC11600.010.5702313
GGCGAGG4000.010.44664719
TGGCGAG10150.010.38445118
GTATCAA23600.010.3539361
TGTAGGA13200.010.2250922
AGGACGT9250.010.17293555
GTCCTAC12350.010.1246921
GGACGTG9250.010.0659056
CGTGGAA5700.09.6612599
GTCCTAA5750.09.6280351
TATACTG3950.09.6253325
TAGGACG10300.09.5972974
TACACCG1002.7409676E-49.5050155
AAGGCGT1002.7526784E-49.5009826
Produced by FastQC (version 0.11.5)