##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511991_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1886317 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.682126068948115 31.0 31.0 34.0 27.0 34.0 2 30.888664524573546 31.0 31.0 34.0 27.0 34.0 3 30.99889520160185 31.0 31.0 34.0 27.0 34.0 4 34.591323197532546 37.0 35.0 37.0 32.0 37.0 5 34.22779734265237 37.0 35.0 37.0 30.0 37.0 6 34.168343390851064 37.0 35.0 37.0 30.0 37.0 7 34.02815115380925 37.0 35.0 37.0 30.0 37.0 8 33.92399527756999 37.0 35.0 37.0 30.0 37.0 9 35.386729802042815 39.0 35.0 39.0 30.0 39.0 10 35.18058576580712 38.0 35.0 39.0 29.0 39.0 11 35.194466253551234 38.0 35.0 39.0 29.0 39.0 12 35.003861493057634 38.0 35.0 39.0 28.0 39.0 13 34.98098940952131 38.0 35.0 39.0 28.0 39.0 14 35.9485584872532 39.0 36.0 41.0 27.0 41.0 15 35.86167860439152 39.0 36.0 41.0 27.0 41.0 16 35.74968311264755 39.0 36.0 41.0 27.0 41.0 17 35.6650160073837 39.0 36.0 40.0 27.0 41.0 18 35.56453448704539 39.0 36.0 40.0 27.0 41.0 19 35.588227217376506 39.0 36.0 40.0 27.0 41.0 20 35.45324672364189 39.0 35.0 40.0 27.0 41.0 21 35.335717697502595 39.0 35.0 40.0 27.0 41.0 22 35.1841403115171 39.0 35.0 40.0 27.0 41.0 23 35.295692081447605 39.0 35.0 40.0 27.0 41.0 24 35.18156598281201 39.0 35.0 40.0 27.0 41.0 25 34.66144767820043 38.0 34.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1937018062686704 1101 2 0.29713819821162346 1101 3 0.29793700789581834 1101 4 0.16127635628681958 1101 5 0.37825102296729796 1101 6 0.40614037517160995 1101 7 0.10480271175388367 1101 8 0.29024659006355336 1101 9 0.12682068532513568 1101 10 0.0973490755411035 1101 11 0.04064632191403916 1101 12 0.35708724325002805 1101 13 0.1455568259888409 1101 14 0.051114051460622534 1101 15 0.10864750650319621 1101 16 0.3737843775932035 1101 17 0.3307554252751146 1101 18 0.30907679868001026 1101 19 0.23453263292509519 1101 20 0.32104146325424665 1101 21 0.29490304114884935 1101 22 0.27226296676104056 1101 23 0.06423951118421911 1101 24 0.10358759851813915 1101 25 -0.05136518064651341 1102 1 0.2955693784248652 1102 2 0.38315970358796747 1102 3 0.4060298205726731 1102 4 0.3463357790372399 1102 5 0.6017937507545099 1102 6 0.5074420163713853 1102 7 0.473223764385466 1102 8 0.5290694536741896 1102 9 0.5591919360325548 1102 10 0.6768623749185778 1102 11 0.6252529998993239 1102 12 0.7837991843932102 1102 13 0.8224102498711261 1102 14 0.8275713236734177 1102 15 0.7017431488348365 1102 16 0.7954029401285752 1102 17 0.9215873437810558 1102 18 0.8733099622340532 1102 19 0.8174415180411145 1102 20 0.8711263528977042 1102 21 0.9536466744255918 1102 22 1.2434004879834504 1102 23 0.9131359458191923 1102 24 0.9332423806347236 1102 25 1.0012663983008565 1103 1 0.1587207252002294 1103 2 0.12291565170940189 1103 3 0.11522643247031894 1103 4 0.1720571071509127 1103 5 0.016195524316643173 1103 6 0.2224055114861372 1103 7 0.06484143826892108 1103 8 0.21794680567857228 1103 9 0.2842458397868697 1103 10 0.18647057296634983 1103 11 0.3739403674784967 1103 12 0.4407028404942679 1103 13 0.3052812503528699 1103 14 0.4912811180206518 1103 15 0.5608216635700884 1103 16 0.5909587451302869 1103 17 0.7364961166463004 1103 18 0.4472004335469677 1103 19 0.674650122062495 1103 20 0.5690726315508883 1103 21 0.6434061303835321 1103 22 0.5685367089677982 1103 23 0.571097565410696 1103 24 0.5480660300007401 1103 25 0.8314712091817 1104 1 0.16940129420562045 1104 2 0.1453433431871467 1104 3 0.26359144845535454 1104 4 0.3161479499138693 1104 5 0.35837258457338095 1104 6 0.27410859219840944 1104 7 0.23582163274247847 1104 8 0.33734330975331517 1104 9 0.3547736776282946 1104 10 0.22762220308631242 1104 11 0.5045834390817276 1104 12 0.6668758503240753 1104 13 0.5573610738320838 1104 14 0.6072506779125817 1104 15 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2315 3 -1.1825560343643744 2315 4 -0.8932809933902846 2315 5 -1.0074216923373243 2315 6 -0.9517656000157331 2315 7 -1.0133251404779244 2315 8 -1.1710417256697312 2315 9 -1.0773078010704467 2315 10 -1.2264204259981497 2315 11 -1.1163357007472854 2315 12 -1.1118126431624091 2315 13 -1.1140064037510342 2315 14 -1.275340611850595 2315 15 -1.4097701344878644 2315 16 -1.386502521305836 2315 17 -1.3367195995094505 2315 18 -1.336888033326801 2315 19 -1.0944763457100066 2315 20 -1.4495116640160006 2315 21 -1.358352265959006 2315 22 -1.4497975035637083 2315 23 -1.4623002424551999 2315 24 -1.3110551625827327 2315 25 -1.3826780200545699 2316 1 -1.1816744980563954 2316 2 -1.032279476063966 2316 3 -1.2715013950920842 2316 4 -1.3708013260204837 2316 5 -1.390680560353779 2316 6 -1.377311909691791 2316 7 -1.5262953931063592 2316 8 -1.66653006866418 2316 9 -1.790320130755255 2316 10 -1.8668387605218228 2316 11 -1.91948483622987 2316 12 -1.869683544109698 2316 13 -1.8145218476916583 2316 14 -2.2372896653936465 2316 15 -2.0051682332692877 2316 16 -2.068222808537108 2316 17 -2.083195369215076 2316 18 -2.15212989335415 2316 19 -2.0940068543441086 2316 20 -2.0205571127733464 2316 21 -1.8739866606307203 2316 22 -1.852179286412877 2316 23 -2.1523429285215983 2316 24 -2.1445385338407874 2316 25 -2.2351270708293995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32855.0 3 1.0 4 625.0 5 4759.0 6 6226.0 7 5017.0 8 4165.0 9 3775.0 10 3454.0 11 3349.0 12 3297.0 13 3028.0 14 3122.0 15 3078.0 16 3176.0 17 3391.0 18 3541.0 19 4024.0 20 4862.0 21 6220.0 22 7865.0 23 9871.0 24 11880.0 25 14060.0 26 16657.0 27 18768.0 28 22405.0 29 26594.0 30 33653.0 31 44174.0 32 57067.0 33 75294.0 34 99155.0 35 137662.0 36 213897.0 37 393575.0 38 601775.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.669621033730266 20.65841701732469 14.83898486499267 23.832977083952375 2 14.96736762696832 21.421319958416323 40.72014406910397 22.891168345511385 3 18.842379091107166 25.453834111657798 30.517458094265177 25.186328702969863 4 13.0057673233078 18.422248222329547 38.623412713769746 29.94857174059291 5 13.242121497220474 37.900819591275656 35.97297162561639 12.88408728588748 6 30.68051930417865 38.51431735644322 18.307875010143903 12.497288329234223 7 27.44655325695522 32.07567975054034 22.64306582615753 17.834701166346907 8 24.606362557300816 37.515009407220525 20.664713301104744 17.213914734373915 9 25.668326161509437 16.383301428126874 20.87305580133138 37.07531660903231 10 14.425518086302565 30.028091778847354 34.56142313301529 20.984967001834793 11 33.58433391630357 23.548905088593276 24.548737036245765 18.318023958857392 12 22.63521984904976 25.589336256843364 31.881915923993688 19.89352797011319 13 28.08350876337328 22.54902012758195 26.291604221347736 23.075866887697032 14 20.598764682712396 22.639885024627358 28.286284860922105 28.475065431738145 15 22.61306026505619 30.81502207741329 24.10729479721595 22.46462286031457 16 21.824168472213312 29.346286970853786 27.492833919219308 21.336710637713598 17 21.445122956533815 29.11265709846224 27.960199690720067 21.482020254283878 18 21.10440609929296 28.531842739051815 29.650318583779928 20.7134325778753 19 22.70376612202509 27.797077585580794 28.384783681639934 21.114372610754184 20 22.819294328203757 27.822803814419217 28.796977812837298 20.56092404453973 21 22.674290694512113 27.417448922954097 27.99471138732249 21.913548995211304 22 22.461548085502063 28.174002566906836 28.34740926366035 21.01704008393075 23 22.05159578162101 27.991742639227656 28.302400922008335 21.65426065714299 24 22.197587924033762 27.578609300207447 29.010067016247504 21.213735759511287 25 22.277680222595478 27.851739451315723 28.447356254559647 21.423224071529155 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 104.0 1 104.0 2 280.5 3 457.0 4 457.0 5 457.0 6 1235.0 7 2013.0 8 2013.0 9 2013.0 10 3341.5 11 4670.0 12 4670.0 13 4670.0 14 7965.0 15 11260.0 16 11260.0 17 11260.0 18 19535.0 19 27810.0 20 27810.0 21 27810.0 22 42662.5 23 57515.0 24 57515.0 25 57515.0 26 78919.5 27 100324.0 28 100324.0 29 100324.0 30 125661.5 31 150999.0 32 150999.0 33 150999.0 34 171412.0 35 191825.0 36 191825.0 37 191825.0 38 206938.5 39 222052.0 40 222052.0 41 222052.0 42 231729.0 43 241406.0 44 241406.0 45 241406.0 46 242549.5 47 243693.0 48 243693.0 49 243693.0 50 232867.0 51 222041.0 52 222041.0 53 222041.0 54 195089.5 55 168138.0 56 168138.0 57 168138.0 58 141485.0 59 114832.0 60 114832.0 61 114832.0 62 91774.5 63 68717.0 64 68717.0 65 68717.0 66 51941.0 67 35165.0 68 35165.0 69 35165.0 70 25152.0 71 15139.0 72 15139.0 73 15139.0 74 10311.0 75 5483.0 76 5483.0 77 5483.0 78 3719.5 79 1956.0 80 1956.0 81 1956.0 82 1246.5 83 537.0 84 537.0 85 537.0 86 338.5 87 140.0 88 140.0 89 140.0 90 90.0 91 40.0 92 40.0 93 40.0 94 20.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4463194680427521 2 0.0 3 0.0 4 0.0 5 0.03938892561536581 6 0.05025666417680591 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 5.301335883629316E-5 21 0.0 22 0.0 23 0.0 24 0.003074774812505003 25 0.010867738561440098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1886317.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.57246644421821 #Duplication Level Percentage of deduplicated Percentage of total 1 84.36748071212972 64.6022608581278 2 11.172270178303206 17.109765666677244 3 2.60549347975655 5.985271861478634 4 0.8815542259405287 2.7001112553835958 5 0.3560481330811323 1.3631741861440774 6 0.18513985855035256 0.8505969363800507 7 0.10177952313172292 0.5455456383798669 8 0.07001067829410663 0.428871225152995 9 0.04516136749664429 0.3112305567490622 >10 0.19447226958788205 2.5078111313521063 >50 0.010344251970597542 0.5511164969471262 >100 0.008792356390864512 1.3968437948728893 >500 6.226994428896422E-4 0.3575592618749251 >1k 7.610770968651153E-4 1.0205988473490202 >5k 6.918882698773775E-5 0.2692422831305918 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5082 0.2694138896060418 No Hit GGTATCAACGCAGAGTACTTTTTTT 3149 0.16693906697548716 No Hit TATCAACGCAGAGTACTTTTTTTTT 3044 0.16137266429767635 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 5.301335883629316E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGTAT 425 0.0 12.127808 1 GGTATCA 1125 0.0 11.963109 1 TGGTATC 420 0.0 11.768114 2 TAGGACC 530 0.0 11.119075 4 GACGTGG 490 0.0 11.044838 7 TCCAACG 105 3.4857785E-6 10.852219 18 GTAGGAC 1160 0.0 10.570231 3 GGCGAGG 400 0.0 10.446647 19 TGGCGAG 1015 0.0 10.384451 18 GTATCAA 2360 0.0 10.353936 1 TGTAGGA 1320 0.0 10.225092 2 AGGACGT 925 0.0 10.1729355 5 GTCCTAC 1235 0.0 10.124692 1 GGACGTG 925 0.0 10.065905 6 CGTGGAA 570 0.0 9.661259 9 GTCCTAA 575 0.0 9.628035 1 TATACTG 395 0.0 9.625332 5 TAGGACG 1030 0.0 9.597297 4 TACACCG 100 2.7409676E-4 9.505015 5 AAGGCGT 100 2.7526784E-4 9.500982 6 >>END_MODULE