FastQCFastQC Report
Thu 26 May 2016
SRR1511990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006566
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35520.177018847124889No Hit
GGTATCAACGCAGAGTACTTTTTTT21340.10635085015892824No Hit
TATCAACGCAGAGTACTTTTTTTTT21230.10580264990037708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG402.8394355E-416.5645394
GGTATCA8500.012.8022781
GTATCAA20300.011.1074661
AGAACCG951.4166861E-510.9599955
GCGTGCG1303.4473487E-810.9216749
CTAATAC1408.8439265E-910.8176573
GTATTAT2500.010.1956881
CGTGCGC1304.039266E-710.193562510
GTACTAT1656.1045284E-910.1006231
ATAGGGG750.002729387510.096483
TAGGACT2350.010.0696284
GCGCCAC2107.2759576E-129.91618613
GCTAGAC1151.072032E-59.8769923
GGATTAT1905.9480953E-109.8035461
GTATAAT2750.09.62531
AAGGCGT2401.8189894E-129.465456
GTCTTAG2405.456968E-129.3950641
GTGGTAT3150.09.3367111
GCGTAGA950.00136938389.287571
TCCTATA1802.5303962E-89.2589042
Produced by FastQC (version 0.11.5)