##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511990_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2006566 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.568024674992 31.0 30.0 34.0 25.0 34.0 2 26.60496240841318 31.0 25.0 34.0 10.0 34.0 3 28.58471836959263 31.0 28.0 34.0 16.0 34.0 4 33.116496541853095 35.0 33.0 37.0 28.0 37.0 5 33.56919732518143 35.0 35.0 37.0 28.0 37.0 6 33.8572830397804 37.0 35.0 37.0 30.0 37.0 7 33.92833627201896 37.0 35.0 37.0 30.0 37.0 8 33.97668255118446 37.0 35.0 37.0 30.0 37.0 9 35.611301596857516 39.0 37.0 39.0 30.0 39.0 10 35.52656777798487 39.0 37.0 39.0 30.0 39.0 11 35.53939765749046 39.0 37.0 39.0 30.0 39.0 12 35.43298301675599 39.0 37.0 39.0 30.0 39.0 13 35.42871353346962 39.0 37.0 39.0 30.0 39.0 14 36.65585732041707 40.0 37.0 41.0 30.0 41.0 15 36.57250945147082 40.0 37.0 41.0 30.0 41.0 16 36.51657608072697 40.0 37.0 41.0 30.0 41.0 17 36.471771175231716 40.0 37.0 41.0 30.0 41.0 18 36.418688944196205 40.0 37.0 41.0 30.0 41.0 19 36.444087560538755 40.0 37.0 41.0 29.0 41.0 20 36.33723136941421 40.0 37.0 41.0 29.0 41.0 21 36.283915405723015 40.0 37.0 41.0 29.0 41.0 22 36.18470062783881 40.0 37.0 41.0 29.0 41.0 23 36.08649752861356 40.0 37.0 41.0 28.0 41.0 24 35.98028821379411 40.0 37.0 41.0 28.0 41.0 25 35.876085810284835 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1595467498654344 1101 2 3.956820959331438 1101 3 2.1376661769217833 1101 4 1.2284149066830068 1101 5 0.8087158789081528 1101 6 0.6703234762363834 1101 7 0.334930446437518 1101 8 0.1223502599502524 1101 9 0.2624509603471594 1101 10 0.5335695429324545 1101 11 0.44207020170550493 1101 12 0.4088308829162557 1101 13 0.5255306051457254 1101 14 0.30651664013304725 1101 15 0.514110072707993 1101 16 0.597885407847059 1101 17 0.547676557469714 1101 18 0.6373000628745089 1101 19 0.480772743575983 1101 20 0.4521269989974641 1101 21 0.40599020849958123 1101 22 0.3742353706006085 1101 23 0.44147561351117304 1101 24 0.35566606385778954 1101 25 0.023006931109975426 1102 1 1.4560546358717197 1102 2 4.179998944574624 1102 3 2.4621739997817293 1102 4 1.5992557487637313 1102 5 1.0978647876908383 1102 6 0.8935421422942582 1102 7 0.7444605648126199 1102 8 0.7908368364957568 1102 9 0.8596252831026732 1102 10 0.6988441566468708 1102 11 0.8965259045485467 1102 12 0.8064621665841898 1102 13 0.8177251104427299 1102 14 0.9990255413381774 1102 15 0.9766970810773117 1102 16 0.9528249087966856 1102 17 0.8820837833538846 1102 18 1.0427287050824887 1102 19 1.0107842556393578 1102 20 1.1101719640483907 1102 21 1.0941845146427482 1102 22 0.9861495061515129 1102 23 1.0527831798998406 1102 24 0.9744279341552087 1102 25 1.0227397735210033 1103 1 1.0698736898685866 1103 2 3.8177331549907976 1103 3 2.220323987457924 1103 4 1.3211987476801639 1103 5 0.8603926325132107 1103 6 0.8304896056832547 1103 7 0.5163614767741507 1103 8 0.3547532020430495 1103 9 0.4305877257272215 1103 10 0.39303592434548307 1103 11 0.5441034631331192 1103 12 0.47120701732663406 1103 13 0.5219915882994073 1103 14 0.5822809789262422 1103 15 0.7664866928077103 1103 16 0.7395870500693391 1103 17 0.7473997145452032 1103 18 0.8060907319530344 1103 19 0.76615906206262 1103 20 0.8332124791369182 1103 21 0.9934699665078242 1103 22 0.9207199866868763 1103 23 0.9746967644527587 1103 24 1.0457891564905921 1103 25 1.0444133926735901 1104 1 1.3445880976272662 1104 2 3.8393341768200244 1104 3 2.2300925545062746 1104 4 1.602087264305787 1104 5 1.147396743570269 1104 6 0.9478319468959739 1104 7 0.8322106967767198 1104 8 0.9801613402307439 1104 9 0.8436779065033946 1104 10 0.8307882678486251 1104 11 0.8320354459532808 1104 12 0.9114478961800216 1104 13 0.8644498298904679 1104 14 0.8259309087925146 1104 15 0.7253008894128712 1104 16 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0.3751590688433879 1107 16 0.3846327818959665 1107 17 0.3683249015659129 1107 18 0.38232270494128073 1107 19 0.37291622708070804 1107 20 0.49741658255674537 1107 21 0.46015322593034824 1107 22 0.367354187904823 1107 23 0.4274175850741102 1107 24 0.3595271234814419 1107 25 0.38295257270968364 1108 1 1.2553611795480215 1108 2 3.4564819434461214 1108 3 2.131475882253966 1108 4 1.5105886077991073 1108 5 1.1556759843936462 1108 6 0.7576578219305148 1108 7 0.54477294726032 1108 8 0.45895501846262476 1108 9 0.5073547860711187 1108 10 0.532670187427172 1108 11 0.43725419723944725 1108 12 0.42680914007256376 1108 13 0.5428735329931413 1108 14 0.3860301861871349 1108 15 0.5814883082006119 1108 16 0.5697434440337474 1108 17 0.6158125012774391 1108 18 0.49109360376263567 1108 19 0.5972577685517138 1108 20 0.6155711920875788 1108 21 0.6032104865446826 1108 22 0.6984673512032771 1108 23 0.6556117947862532 1108 24 0.615762592251798 1108 25 0.5066565751172618 1109 1 0.8788875542898715 1109 2 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-0.7623810750877738 2315 8 -0.8039121620245737 2315 9 -0.9586169847525383 2315 10 -1.0751678971984475 2315 11 -1.0547444408266031 2315 12 -0.9356703535356274 2315 13 -1.0074445095363416 2315 14 -1.053903110002544 2315 15 -1.4302537291287578 2315 16 -1.3436437475965448 2315 17 -1.29388431365431 2315 18 -1.300648897862402 2315 19 -1.1073867357352825 2315 20 -1.1590194539024523 2315 21 -1.1460009846535115 2315 22 -1.4108812419808672 2315 23 -1.3274181987136089 2315 24 -1.223239791132606 2315 25 -1.1805714250065549 2316 1 -0.5868597124526289 2316 2 0.09001113739242683 2316 3 -0.08050925005890619 2316 4 -0.42427193096335003 2316 5 -0.8230419218022575 2316 6 -0.8085756645171216 2316 7 -0.9373044625483828 2316 8 -0.9782836384325932 2316 9 -0.9792920105685425 2316 10 -1.2325922120300348 2316 11 -1.3604092572071522 2316 12 -1.3531176270649539 2316 13 -1.3656503223978902 2316 14 -1.4307576013186747 2316 15 -1.2481209095533785 2316 16 -1.3908019821495543 2316 17 -1.645539879718207 2316 18 -1.6536295183153555 2316 19 -1.4724724715999784 2316 20 -1.439866486148226 2316 21 -1.6302829655847617 2316 22 -1.5405380645906703 2316 23 -1.4940642571363085 2316 24 -1.6307646964739249 2316 25 -1.5187899704209968 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62587.0 3 5.0 4 553.0 5 2585.0 6 2965.0 7 2739.0 8 2645.0 9 2509.0 10 2443.0 11 2418.0 12 2365.0 13 2447.0 14 2508.0 15 2490.0 16 2668.0 17 2929.0 18 3392.0 19 4098.0 20 5150.0 21 6718.0 22 8235.0 23 9971.0 24 11348.0 25 13312.0 26 14743.0 27 17221.0 28 19988.0 29 24774.0 30 30815.0 31 39726.0 32 52247.0 33 70120.0 34 97102.0 35 142576.0 36 230285.0 37 320600.0 38 787289.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.051179976138336 17.777636020943245 12.610001365517007 25.561182637401416 2 18.299966398213684 20.93762920041685 36.73728203357391 24.02512236779555 3 18.88340577882811 23.38422957430755 29.226648911623144 28.505715735241203 4 13.277161080173789 16.123915186442908 36.31223692617138 34.28668680721192 5 14.616862839298584 36.43383771079546 34.57454177933843 14.374757670567526 6 33.593944673581305 35.36079586796135 17.28821009446995 13.757049363987395 7 29.260288472943323 31.094367192507 21.25462107899765 18.39072325555202 8 26.772270023647376 34.45814115166964 19.858065898687556 18.91152292599542 9 26.88284362438116 15.379010707846142 19.343594977688248 38.39455069008445 10 15.959363389673397 27.952047404395074 32.88590202659769 23.202687179333836 11 35.144171684360245 22.483237531185118 22.969989524391423 19.402601260063214 12 23.54863981548576 24.173637946621245 30.050693572999844 22.227028664893155 13 28.935903429042455 20.89854009287509 25.36781745529427 24.797739022788186 14 22.628161744991193 20.20591398438925 26.568824549005615 30.59709972161394 15 24.287464254851322 27.71979591002738 23.187973881746228 24.804765953375068 16 24.389529175716124 27.088319048563562 24.751690201069888 23.770461574650422 17 23.41951373640339 26.84731028034961 26.152939898313836 23.580236084933166 18 23.362555860610986 26.79181304094526 26.590174114166704 23.25545698427705 19 24.350876528492655 26.346189881946742 26.532778617249893 22.77015497231071 20 24.58214514606605 25.944278710109554 26.352639694441926 23.120936449382476 21 24.636370930805693 25.71795092870254 26.26970524428248 23.375972896209287 22 24.66193015033854 25.55740285008308 26.180017522718234 23.600649476860145 23 24.403852977645236 25.56121147122028 26.102388575028208 23.932546976106277 24 23.961683792110502 25.753650764540016 26.318047848912023 23.966617594437462 25 24.30257540139572 25.6181596273425 26.148121088768296 23.93114388249348 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 225.0 1 225.0 2 339.5 3 454.0 4 454.0 5 454.0 6 1062.0 7 1670.0 8 1670.0 9 1670.0 10 2381.0 11 3092.0 12 3092.0 13 3092.0 14 5202.0 15 7312.0 16 7312.0 17 7312.0 18 12928.5 19 18545.0 20 18545.0 21 18545.0 22 27307.5 23 36070.0 24 36070.0 25 36070.0 26 50524.0 27 64978.0 28 64978.0 29 64978.0 30 83543.0 31 102108.0 32 102108.0 33 102108.0 34 122442.5 35 142777.0 36 142777.0 37 142777.0 38 165548.5 39 188320.0 40 188320.0 41 188320.0 42 209087.5 43 229855.0 44 229855.0 45 229855.0 46 245575.5 47 261296.0 48 261296.0 49 261296.0 50 268731.0 51 276166.0 52 276166.0 53 276166.0 54 259966.5 55 243767.0 56 243767.0 57 243767.0 58 215301.0 59 186835.0 60 186835.0 61 186835.0 62 155590.0 63 124345.0 64 124345.0 65 124345.0 66 96620.5 67 68896.0 68 68896.0 69 68896.0 70 50329.5 71 31763.0 72 31763.0 73 31763.0 74 21964.5 75 12166.0 76 12166.0 77 12166.0 78 8125.0 79 4084.0 80 4084.0 81 4084.0 82 2683.0 83 1282.0 84 1282.0 85 1282.0 86 839.5 87 397.0 88 397.0 89 397.0 90 251.5 91 106.0 92 106.0 93 106.0 94 74.5 95 43.0 96 43.0 97 43.0 98 28.5 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.4471330621569387 3 0.0 4 0.0 5 0.0 6 9.967277428203209E-5 7 0.0 8 4.9836387141016045E-5 9 0.0 10 4.9836387141016045E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.485274842691444E-4 19 6.977094199742246E-4 20 3.9869109712812836E-4 21 0.0028406740670379143 22 0.0013954188399484492 23 6.977094199742246E-4 24 0.0 25 2.491819357050802E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2006566.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.5152401280701 #Duplication Level Percentage of deduplicated Percentage of total 1 74.74952838004604 48.224837728986614 2 15.084702468550532 19.463864040380585 3 5.167552654226676 10.0015770108564 4 2.1245864584003193 5.482728221461705 5 1.0275255252448494 3.3145527999446407 6 0.5841385414911506 2.2611502963417336 7 0.3490807109861758 1.57647181253454 8 0.21273216358153457 1.0979573293141274 9 0.14151711071414982 0.8217009341958651 >10 0.5368716567285844 5.533339990468293 >50 0.015043346663363627 0.6583536424737274 >100 0.005627722527757422 0.670279356086257 >500 6.247204793832798E-4 0.32147471411884976 >1k 4.6854035953745914E-4 0.5717121228366563 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3552 0.177018847124889 No Hit GGTATCAACGCAGAGTACTTTTTTT 2134 0.10635085015892824 No Hit TATCAACGCAGAGTACTTTTTTTTT 2123 0.10580264990037708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.967277428203209E-5 2 0.0 0.0 0.0 0.0 9.967277428203209E-5 3 0.0 0.0 0.0 0.0 9.967277428203209E-5 4 0.0 0.0 0.0 0.0 9.967277428203209E-5 5 0.0 0.0 0.0 0.0 9.967277428203209E-5 6 0.0 0.0 0.0 0.0 1.4950916142304814E-4 7 0.0 0.0 0.0 4.9836387141016045E-5 1.4950916142304814E-4 8 0.0 0.0 0.0 4.9836387141016045E-5 1.4950916142304814E-4 9 0.0 0.0 0.0 4.9836387141016045E-5 1.4950916142304814E-4 10 0.0 0.0 0.0 4.9836387141016045E-5 1.4950916142304814E-4 11 0.0 0.0 0.0 4.9836387141016045E-5 1.4950916142304814E-4 12 0.0 0.0 0.0 4.9836387141016045E-5 4.485274842691444E-4 13 0.0 0.0 0.0 4.9836387141016045E-5 4.485274842691444E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGACG 40 2.8394355E-4 16.564539 4 GGTATCA 850 0.0 12.802278 1 GTATCAA 2030 0.0 11.107466 1 AGAACCG 95 1.4166861E-5 10.959995 5 GCGTGCG 130 3.4473487E-8 10.921674 9 CTAATAC 140 8.8439265E-9 10.817657 3 GTATTAT 250 0.0 10.195688 1 CGTGCGC 130 4.039266E-7 10.1935625 10 GTACTAT 165 6.1045284E-9 10.100623 1 ATAGGGG 75 0.0027293875 10.09648 3 TAGGACT 235 0.0 10.069628 4 GCGCCAC 210 7.2759576E-12 9.916186 13 GCTAGAC 115 1.072032E-5 9.876992 3 GGATTAT 190 5.9480953E-10 9.803546 1 GTATAAT 275 0.0 9.6253 1 AAGGCGT 240 1.8189894E-12 9.46545 6 GTCTTAG 240 5.456968E-12 9.395064 1 GTGGTAT 315 0.0 9.336711 1 GCGTAGA 95 0.0013693838 9.28757 1 TCCTATA 180 2.5303962E-8 9.258904 2 >>END_MODULE