FastQCFastQC Report
Thu 26 May 2016
SRR1511990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006566
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44230.22042634032471398No Hit
TATCAACGCAGAGTACTTTTTTTTT27730.1381963015420375No Hit
GGTATCAACGCAGAGTACTTTTTTT27730.1381963015420375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT1850.012.8981061
GGTATCA10850.012.7554531
TCCGCGC701.0964431E-412.20802216
GTTATAA2150.011.5423051
GAACCGC600.00587730111.0831736
GGGTCGT700.001498126810.8515757
TGGTATC3800.010.754942
CTAGTAC803.755728E-410.692413
GTGGTAT4400.010.412291
GTATCAA24150.010.3548021
GTCCTAA1409.0491085E-810.2263551
TAGACTG2105.456968E-129.9569535
TTAGACC1054.085154E-59.9569534
GGCTAAT1606.173104E-89.5445991
AGAACCG1002.743049E-49.5043655
TATTATA2800.09.5043652
TAGTACT1401.0520544E-69.5043654
TTATACC1201.6913986E-59.5043644
ACACCGT1502.648976E-79.4998646
GAACCGG900.00111395759.4998636
Produced by FastQC (version 0.11.5)