FastQCFastQC Report
Thu 26 May 2016
SRR1511989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994566
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32170.16128822009399538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9050.011.8056721
GGCGAGG4850.011.12504119
CCAACGC600.00603760511.0431633
AAGGCCG803.9003493E-410.6487655
AGGACGT10200.010.4863665
GTGTAAG2550.010.3785441
TAGGACC3600.010.2543664
TAGGACG10900.010.2471294
GACGTGG5150.010.1088597
GTATCAA18250.010.043651
ACCGTCC856.818482E-410.02236758
TTAGGAC7000.010.0064583
CCTACAG11200.09.9726523
TATACTG2950.09.9468695
GGACGTG10600.09.8227616
GTCCTAC9700.09.7009061
GTAGGAC9800.09.6587443
CTACAGT12400.09.54190354
TCCTACA11200.09.5394052
TTATACT1901.1223165E-99.4655694
Produced by FastQC (version 0.11.5)