FastQCFastQC Report
Thu 26 May 2016
SRR1511989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994566
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40260.2018484221630169No Hit
TATCAACGCAGAGTACTTTTTTTTT25590.12829858726158974No Hit
GGTATCAACGCAGAGTACTTTTTTT23970.12017651960376342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8750.012.3278071
GGACGTG9500.011.1001616
CGAATTT600.00589908911.07765915
TACCGAA600.00589908911.0776597
TAGGACC3900.010.9663734
CGTGCGC700.001498109110.85158410
GACGTGA5800.010.4773927
AGGACGT10050.010.4025965
ACTAGAC1106.0150887E-610.3682083
GTATCAA18200.010.3326151
GTATAAG1855.4569682E-1110.3198581
GACGTGG4700.010.3032337
GTCCTAG2250.010.1822591
ATAGGAC2450.010.086083
GTCCTAA5600.09.8867921
GTAGGAC11050.09.8052283
TGTAGGA11950.09.7825562
TAGGACG10600.09.7731774
GTCCTAC8700.09.7653131
TTAGGAC6850.09.7123123
Produced by FastQC (version 0.11.5)