FastQCFastQC Report
Thu 26 May 2016
SRR1511988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887623
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32800.17376351104007529No Hit
TATCAACGCAGAGTACTTTTTTTTT20240.10722480071497327No Hit
GGTATCAACGCAGAGTACTTTTTTT19730.1045229900250209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT400.005409027414.198096
GGTATCA8850.012.6285821
TAGGACC5050.012.3706124
CGGTAGG1103.3067045E-711.5862691
GACGAAA1201.3411227E-711.0432519
AACCCGT600.006038238311.0429586
CGGTTTC750.002728799410.09668513
GGCGAGG5350.010.08489119
CGTGCGC951.7059117E-49.96357210
GTCTTAG2500.09.8037671
TAATACC1856.9303496E-109.7212144
GTAGGAC13000.09.6838253
TGGCGAG11300.09.63317618
GTATCAA16350.09.5339381
GGACGTG12550.09.5031036
ATAATAC2201.8189894E-119.4653933
GACGTGA7300.09.4653937
GGCGTGC1107.1151764E-59.4653938
AGGACGT12750.09.4282745
GTCCTAG2208.913048E-119.3581411
Produced by FastQC (version 0.11.5)