FastQCFastQC Report
Thu 26 May 2016
SRR1511988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887623
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40420.2141317413487757No Hit
TATCAACGCAGAGTACTTTTTTTTT26130.13842806534991361No Hit
GGTATCAACGCAGAGTACTTTTTTT25730.13630899814210784No Hit
CTGTAGGACGTGGAATATGGCAAGA20560.10892005448121791No Hit
GTCCTACAGTGGACATTTCTAAATT18990.10060271569058016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG707.2428847E-613.5753485
GGTATCA10400.013.02946951
CCATGCG550.003078686512.0857529
GCGCCAC1001.9400413E-611.39513713
TTAGACA2000.010.9281564
TCGAACT1451.4519173E-810.47884219
GTCCTAG2850.010.379791
GACGTGG5050.010.3421217
GTCCTAA7350.010.2567911
AGGACGT10550.010.1782945
CTATTAG1605.7334546E-910.139131
ATTAAGC1502.4339897E-810.136263
GGACGTG10350.010.0952536
AGAACCG856.576855E-410.06172855
TAGGACC3700.010.0164054
GTATAGT2201.8189894E-129.976471
ATAGGAC2201.8189894E-129.9346883
GTATAGA1356.0594357E-79.8961441
AAGGCGT1951.6370905E-109.7422726
CGTGCGC1304.261632E-69.49594710
Produced by FastQC (version 0.11.5)