FastQCFastQC Report
Thu 26 May 2016
SRR1511987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94295
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA33773.581313961503791No Hit
GTATCAACGCAGAGTACTTTTTTTT21932.3256800466620713No Hit
GCTTATGTACTCTGCGTTGATACCA16671.7678561959806989No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA16251.7233151280555703No Hit
GGTATCAACGCAGAGTACTTTTTTT15941.6904395779203563No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT14421.5292433320960814No Hit
TATCAACGCAGAGTACTTTTTTTTT14401.5271223288615516No Hit
CTTATGTACTCTGCGTTGATACCAC11361.2047298372130018No Hit
GAGTACATAAGCAGTGGTATCAACG8580.9099103876133411No Hit
TATGTACTCTGCGTTGATACCACTG7840.8314332679357337No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7750.8218887533803488No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6600.6999310673948778No Hit
CATAAGCAGTGGTATCAACGCAGAG6090.6458454849143644No Hit
GCGTTGATACCACTGCTTATGTACT5400.5726708733230819No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5010.5313113102497481No Hit
CCCATGTACTCTGCGTTGATACCAC4960.5260088021634233No Hit
GGTATCAACGCAGAAAAAAAAAAAA4900.5196457924598336No Hit
GTATCAACGCAGAGTACATAAGCAG4550.4825282358555597No Hit
ACGCAGAGTACATAAGCAGTGGTAT4360.4623787051275253No Hit
TATCAACGCAGAGTACATAAGCAGT4250.4507131873376107No Hit
CTGCTTATGTACTCTGCGTTGATAC3970.42101914205419166No Hit
GGTATCAACGCAGAGTACATAAGCA3820.40511161779521715No Hit
GGTATCAACGCAGAGAAAAAAAAAA3660.3881435919189777No Hit
GTGGTATCAACGCAGAGTACATGGG3220.34148152075931915No Hit
GTATCAACGCAGAGAAAAAAAAAAA3210.3404210191420542No Hit
ACTCTGCGTTGATACCACTGCTTAT3150.33405800943846437No Hit
GTACATGGGAAGCAGTGGTATCAAC3060.32451349488307973No Hit
AAGCAGTGGTATCAACGCAGAGTAC3050.3234529932658147No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2680.28421443342701097No Hit
CATGTACTCTGCGTTGATACCACTG2590.2746699188716263No Hit
ACATAAGCAGTGGTATCAACGCAGA2530.2683069091680365No Hit
GTATCAACGCAGAAAAAAAAAAAAA2340.2481573784400021No Hit
ATCAACGCAGAGTACTTTTTTTTTT2310.24497587358820722No Hit
GTGGTATCAACGCAGAGTACATAAG2210.23437085741555755No Hit
GGTATCAACGCAGAGTACATGGGGG2110.22376584124290788No Hit
GTGGTATCAACGCAGAGTACTTTTT1960.20785831698393342No Hit
GTACATGGGATAAGCAGTGGTATCA1930.20467681213213848No Hit
CCACTGCTTATGTACTCTGCGTTGA1750.1855877830213691No Hit
TATCAACGCAGAGAAAAAAAAAAAA1720.1824062781695742No Hit
GTACTCTGCGTTGATACCACTGCTT1640.1739222652314545No Hit
GGACATAAGCAGTGGTATCAACGCA1600.16968025876239462No Hit
GNACATAAGCAGTGGTATCAACGCA1600.16968025876239462No Hit
GATACCACTGCTTATGTACTCTGCG1570.1664987539105997No Hit
GTTGATACCACTGCTTATGTACTCT1530.16225674744153987No Hit
ATACCACTGCTTATGTACTCTGCGT1430.1516517312688902No Hit
GGTATCAACGCAGAGTACATGGGAA1410.14953072803436024No Hit
GAGTACATGGGAAGCAGTGGTATCA1410.14953072803436024No Hit
GTACTGGTTCACTATCGGTCAGTCA1400.14847022641709529No Hit
GAACATAAGCAGTGGTATCAACGCA1390.14740972479983033No Hit
GCAGTGGTATCAACGCAGAGTACAT1370.14528872156530037No Hit
CAGTGGTATCAACGCAGAGTACATA1280.13574420700991569No Hit
TATCAACGCAGAAAAAAAAAAAAAA1270.1346837053926507No Hit
GGTATCAACGCAGAGTACATGGGGA1240.13150220054085582No Hit
ATACAGGGTGACAGCCCCGTACACA1220.12938119730632588No Hit
GTATCAACGCAGAGTACATGGGGGG1210.12832069568906093No Hit
GTACACAAGCAGTGGTATCAACGCA1200.12726019407179595No Hit
GAATCAACGCAGAGTACTTTTTTTT1180.12513919083726602No Hit
GTATCAACGCAGAGTACATGGGGGT1170.12407868922000105No Hit
GTATCAACGCAGAGTACATGGGAAG1140.12089718436820616No Hit
GNATCAACGCAGAGTACTTTTTTTT1130.11983668275094118No Hit
TGATACCACTGCTTATGTACTCTGC1130.11983668275094118No Hit
GGATAAGCAGTGGTATCAACGCAGA1110.11771567951641126No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1100.1166551778991463No Hit
GGATCAACGCAGAGTACTTTTTTTT1100.1166551778991463No Hit
GCAGAGTACATAAGCAGTGGTATCA1040.1102921681955565No Hit
GCTTATCCCATGTACTCTGCGTTGA1040.1102921681955565No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1020.10817116496102658No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1020.10817116496102658No Hit
TACCACTGCTTATGTACTCTGCGTT1010.1071106633437616No Hit
GNTATCAACGCAGAGTACTTTTTTT970.10286865687470173No Hit
GATTATGTACTCTGCGTTGATACCA970.10286865687470173No Hit
GCGTTGATACCACTGCTTCCCATGT950.1007476536401718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCAA500.001130422213.7843181
GATCAAC500.001130422213.7843182
GTACATA7050.011.8709941
TACATAA6950.011.7584632
ACATAAG7500.011.4774263
AATCAAC802.6552065E-411.0766842
CATAAGC8000.010.7600874
GAATCAA854.6512912E-410.4251141
TAAGCAG8850.09.8335186
ATAAGCA8900.09.7782745
GGAAGCA800.00460933949.4594178
AGCAGTG9450.09.3092688
GCAGTGG9800.09.2663689
CAGTGGT9800.09.26636810
AGTGGTA9900.09.17276811
AAGCAGT9750.09.0228287
GGGAAGC850.00758929558.902987
TGGTATC10400.08.73176913
GTGGTAT10800.08.4083712
GCTTATG4100.08.4050721
Produced by FastQC (version 0.11.5)