##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511987_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94295 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.510323983244074 31.0 30.0 34.0 25.0 34.0 2 26.228941089135162 31.0 25.0 33.0 10.0 34.0 3 28.214539477172703 31.0 27.0 34.0 16.0 34.0 4 32.933230818176995 35.0 33.0 37.0 27.0 37.0 5 33.50216872580731 35.0 35.0 37.0 28.0 37.0 6 33.91276313696378 37.0 35.0 37.0 30.0 37.0 7 34.033066440426325 37.0 35.0 37.0 30.0 37.0 8 34.08062993796066 37.0 35.0 37.0 30.0 37.0 9 35.65402195238348 39.0 37.0 39.0 30.0 39.0 10 35.638591653852274 39.0 37.0 39.0 30.0 39.0 11 35.597285115859805 39.0 37.0 39.0 30.0 39.0 12 35.532096081446525 39.0 37.0 39.0 30.0 39.0 13 35.37438888594305 39.0 37.0 39.0 30.0 39.0 14 36.451179808049204 40.0 37.0 41.0 30.0 41.0 15 36.28400233310356 40.0 36.0 41.0 28.0 41.0 16 36.117567209289994 40.0 36.0 41.0 27.0 41.0 17 36.381526061827245 40.0 37.0 41.0 30.0 41.0 18 36.366679039185534 40.0 36.0 41.0 30.0 41.0 19 36.46720398748608 40.0 36.0 41.0 30.0 41.0 20 36.444318362585506 40.0 36.0 41.0 30.0 41.0 21 36.38864202767909 40.0 36.0 41.0 30.0 41.0 22 36.289188186011984 40.0 36.0 41.0 30.0 41.0 23 36.1142690492603 40.0 36.0 41.0 30.0 41.0 24 36.03441327748025 40.0 35.0 41.0 29.0 41.0 25 35.931968821252454 40.0 35.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4946205098496819 1101 2 4.2812313435277005 1101 3 2.6826311225467556 1101 4 1.60764192110895 1101 5 0.6248202708386827 1101 6 0.5312430460927544 1101 7 0.21415977487215088 1101 8 -0.7903620831006464 1101 9 0.5750622488508839 1101 10 0.6286300557747708 1101 11 0.4680766414311961 1101 12 0.39273196743982197 1101 13 0.451846145398882 1101 14 0.2352657546480046 1101 15 0.2033968546803706 1101 16 0.8578899557993509 1101 17 0.28510053419439174 1101 18 0.6616679621066552 1101 19 0.40894663322428215 1101 20 0.4997417391177521 1101 21 0.18634553149581023 1101 22 -0.2394381565013859 1101 23 -0.470521329561123 1101 24 -0.6296417142684447 1101 25 -0.4149889310277999 1102 1 2.0962817593982557 1102 2 4.673069552267318 1102 3 3.3238373341755008 1102 4 1.9588517441497615 1102 5 1.9151778006328328 1102 6 1.752080893691165 1102 7 1.8091399121058132 1102 8 1.880096566231245 1102 9 1.5515880704471243 1102 10 1.772905967656321 1102 11 2.041568876895262 1102 12 1.3275459797186215 1102 13 1.7375088395122802 1102 14 1.5530700161142406 1102 15 1.926040408309845 1102 16 1.400685188735423 1102 17 1.7117888693334322 1102 18 1.8523505189863911 1102 19 1.8814999015521963 1102 20 2.362688651360436 1102 21 2.4288518514669164 1102 22 2.462078636564705 1102 23 1.9651955357332085 1102 24 1.9536013337633307 1102 25 2.292306121337674 1103 1 1.1387230739522458 1103 2 3.72020570250206 1103 3 1.611349071264705 1103 4 1.0358470493140786 1103 5 0.3540510400694501 1103 6 0.0856020204517236 1103 7 0.4269802876926647 1103 8 -0.20215695489551422 1103 9 -0.451604417815787 1103 10 0.32145056859528864 1103 11 0.3788458722004293 1103 12 -0.010857776149919118 1103 13 -0.08558975203701635 1103 14 0.02706062644287499 1103 15 0.18801223929575883 1103 16 0.6343002122096024 1103 17 0.5020236111174725 1103 18 0.01141155185024445 1103 19 0.47304919732684425 1103 20 0.394613533989542 1103 21 0.26378142893170775 1103 22 0.1385105614473332 1103 23 0.13101713197733744 1103 24 0.6698454652187351 1103 25 1.2891136330747628 1104 1 0.8985499137790853 1104 2 3.410768473064831 1104 3 1.2828642227798532 1104 4 0.9544617679287981 1104 5 0.2641809101993218 1104 6 -0.08530707045736818 1104 7 0.12741318812556557 1104 8 0.617063824325264 1104 9 0.345625019413653 1104 10 0.21720814435285973 1104 11 0.5603177336722922 1104 12 0.1188391935470463 1104 13 0.8011634947162278 1104 14 0.47935499873725007 1104 15 0.7828174341009557 1104 16 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-0.8098923993812193 2315 18 -0.7617118077298102 2315 19 -0.5033287554290595 2315 20 0.09907547624676027 2315 21 -0.534328807288766 2315 22 -0.29970466164977694 2315 23 -0.7032085898074385 2315 24 -0.7703645085345485 2315 25 -0.9230648446155243 2316 1 -0.04997257822166645 2316 2 0.5242636735165505 2316 3 0.6603345785110797 2316 4 0.7865716869952379 2316 5 0.04622495311292596 2316 6 0.14328317987201444 2316 7 0.5023426065332472 2316 8 0.511756088582743 2316 9 0.42288833580740004 2316 10 0.38058100337789824 2316 11 -0.025791808958992135 2316 12 0.19262048471964732 2316 13 -0.13399554913846856 2316 14 -0.09583792428175997 2316 15 0.37786731175952326 2316 16 -0.2987432660512681 2316 17 -0.30464305554919946 2316 18 -0.07467540467149547 2316 19 -0.5776754403543123 2316 20 0.4033091861634617 2316 21 0.22870896516359096 2316 22 0.07995983680964969 2316 23 0.2881185812527036 2316 24 -0.11682120144793373 2316 25 0.3230266765530274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2975.0 3 0.0 4 26.0 5 93.0 6 142.0 7 104.0 8 102.0 9 127.0 10 106.0 11 102.0 12 97.0 13 102.0 14 80.0 15 86.0 16 128.0 17 118.0 18 152.0 19 173.0 20 233.0 21 314.0 22 405.0 23 458.0 24 581.0 25 595.0 26 662.0 27 776.0 28 940.0 29 1041.0 30 1457.0 31 1804.0 32 2447.0 33 3427.0 34 4939.0 35 7488.0 36 12820.0 37 16121.0 38 33074.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.07476536401718 18.41773158704067 14.947770295349699 16.55973275359245 2 16.721126884949108 25.140750226304288 37.856843220806745 20.28127966793986 3 18.986160453894694 33.13537303144387 32.94236173710165 14.936104777559786 4 10.983615250013257 21.006416034784454 35.566042738215174 32.44392597698712 5 8.860491012248794 43.696908637785675 33.122647012036694 14.31995333792884 6 24.658783604644995 35.37621294872474 23.361790126729943 16.603213319900313 7 20.116655177899144 40.98944800890821 21.315022005408558 17.578874807784082 8 23.165597327535924 34.196935150326105 23.814624317302084 18.822843204835888 9 29.503154992311366 17.958534386764942 19.242801845272815 33.29550877565089 10 17.5269102285381 27.773476854552204 27.779839864255795 26.919773052653905 11 26.854021952383476 29.188186011983667 21.486823267405484 22.470968768227372 12 27.452144864520918 29.625112678296833 25.44143379818654 17.481308658995705 13 29.19773052653905 23.59403998091097 31.36009332414232 15.848136168407656 14 28.017392226523146 26.475422875019884 27.30897714619015 18.198207752266825 15 28.94957314809905 25.75322127366244 28.196616999840924 17.100588578397584 16 19.663820987327004 29.655867225197518 31.588101171854287 19.092210615621187 17 16.46216660480407 34.62325680046662 29.045018293652898 19.86955830107641 18 15.604551769484479 26.786717996033637 40.445208021804376 17.163522212677503 19 18.73329656810758 32.172400627836936 31.4660840792432 17.628218724812285 20 19.0183788828439 29.788001230207968 35.160616376613326 16.033003510334805 21 20.792236716512885 31.415844734330257 31.11358574610245 16.678332803054406 22 18.277072529243952 29.937535129861175 30.055253306183914 21.730139034710955 23 21.832180884910706 26.742459593602852 31.782123616001357 19.643235905485092 24 16.781377591600826 29.878572564823163 29.908266610106583 23.43178323346943 25 19.71578556657299 32.138501511214805 31.416299909857365 16.729413012354843 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3375.0 1 3375.0 2 2062.5 3 750.0 4 750.0 5 750.0 6 918.5 7 1087.0 8 1087.0 9 1087.0 10 946.0 11 805.0 12 805.0 13 805.0 14 705.5 15 606.0 16 606.0 17 606.0 18 1146.5 19 1687.0 20 1687.0 21 1687.0 22 2435.0 23 3183.0 24 3183.0 25 3183.0 26 4450.5 27 5718.0 28 5718.0 29 5718.0 30 6219.0 31 6720.0 32 6720.0 33 6720.0 34 6524.5 35 6329.0 36 6329.0 37 6329.0 38 6535.0 39 6741.0 40 6741.0 41 6741.0 42 13110.0 43 19479.0 44 19479.0 45 19479.0 46 16319.5 47 13160.0 48 13160.0 49 13160.0 50 11480.5 51 9801.0 52 9801.0 53 9801.0 54 8059.5 55 6318.0 56 6318.0 57 6318.0 58 5008.5 59 3699.0 60 3699.0 61 3699.0 62 3129.5 63 2560.0 64 2560.0 65 2560.0 66 1931.5 67 1303.0 68 1303.0 69 1303.0 70 955.5 71 608.0 72 608.0 73 608.0 74 432.0 75 256.0 76 256.0 77 256.0 78 163.5 79 71.0 80 71.0 81 71.0 82 49.0 83 27.0 84 27.0 85 27.0 86 18.0 87 9.0 88 9.0 89 9.0 90 5.5 91 2.0 92 2.0 93 2.0 94 1.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.93340049843576 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0021210032345299326 19 0.003181504851794899 20 0.0021210032345299326 21 0.005302508086324832 22 0.0021210032345299326 23 0.003181504851794899 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 94295.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.852590275200164 #Duplication Level Percentage of deduplicated Percentage of total 1 80.44652598525772 32.0600243915372 2 10.902365682961229 8.689750251869134 3 3.501955879613614 4.186860384962087 4 1.4955161127225312 2.384007635611644 5 0.8408951808190744 1.6755925552786468 6 0.45504137949386625 1.0880746593138555 7 0.32731046595172836 0.9130918924651361 8 0.19691849171079592 0.6278169574208601 9 0.12506985284334335 0.44859218410308077 >10 1.3491577742888314 10.821358502571716 >50 0.1756300061204396 4.838008377962776 >100 0.14369727773490515 11.904130653799246 >500 0.018627424891561776 5.012991144811496 >1k 0.021288485590356315 15.349700408293124 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATAAGCAGTGGTATCAACGCA 3377 3.581313961503791 No Hit GTATCAACGCAGAGTACTTTTTTTT 2193 2.3256800466620713 No Hit GCTTATGTACTCTGCGTTGATACCA 1667 1.7678561959806989 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1625 1.7233151280555703 No Hit GGTATCAACGCAGAGTACTTTTTTT 1594 1.6904395779203563 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 1442 1.5292433320960814 No Hit TATCAACGCAGAGTACTTTTTTTTT 1440 1.5271223288615516 No Hit CTTATGTACTCTGCGTTGATACCAC 1136 1.2047298372130018 No Hit GAGTACATAAGCAGTGGTATCAACG 858 0.9099103876133411 No Hit TATGTACTCTGCGTTGATACCACTG 784 0.8314332679357337 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 775 0.8218887533803488 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 660 0.6999310673948778 No Hit CATAAGCAGTGGTATCAACGCAGAG 609 0.6458454849143644 No Hit GCGTTGATACCACTGCTTATGTACT 540 0.5726708733230819 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 501 0.5313113102497481 No Hit CCCATGTACTCTGCGTTGATACCAC 496 0.5260088021634233 No Hit GGTATCAACGCAGAAAAAAAAAAAA 490 0.5196457924598336 No Hit GTATCAACGCAGAGTACATAAGCAG 455 0.4825282358555597 No Hit ACGCAGAGTACATAAGCAGTGGTAT 436 0.4623787051275253 No Hit TATCAACGCAGAGTACATAAGCAGT 425 0.4507131873376107 No Hit CTGCTTATGTACTCTGCGTTGATAC 397 0.42101914205419166 No Hit GGTATCAACGCAGAGTACATAAGCA 382 0.40511161779521715 No Hit GGTATCAACGCAGAGAAAAAAAAAA 366 0.3881435919189777 No Hit GTGGTATCAACGCAGAGTACATGGG 322 0.34148152075931915 No Hit GTATCAACGCAGAGAAAAAAAAAAA 321 0.3404210191420542 No Hit ACTCTGCGTTGATACCACTGCTTAT 315 0.33405800943846437 No Hit GTACATGGGAAGCAGTGGTATCAAC 306 0.32451349488307973 No Hit AAGCAGTGGTATCAACGCAGAGTAC 305 0.3234529932658147 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 268 0.28421443342701097 No Hit CATGTACTCTGCGTTGATACCACTG 259 0.2746699188716263 No Hit ACATAAGCAGTGGTATCAACGCAGA 253 0.2683069091680365 No Hit GTATCAACGCAGAAAAAAAAAAAAA 234 0.2481573784400021 No Hit ATCAACGCAGAGTACTTTTTTTTTT 231 0.24497587358820722 No Hit GTGGTATCAACGCAGAGTACATAAG 221 0.23437085741555755 No Hit GGTATCAACGCAGAGTACATGGGGG 211 0.22376584124290788 No Hit GTGGTATCAACGCAGAGTACTTTTT 196 0.20785831698393342 No Hit GTACATGGGATAAGCAGTGGTATCA 193 0.20467681213213848 No Hit CCACTGCTTATGTACTCTGCGTTGA 175 0.1855877830213691 No Hit TATCAACGCAGAGAAAAAAAAAAAA 172 0.1824062781695742 No Hit GTACTCTGCGTTGATACCACTGCTT 164 0.1739222652314545 No Hit GGACATAAGCAGTGGTATCAACGCA 160 0.16968025876239462 No Hit GNACATAAGCAGTGGTATCAACGCA 160 0.16968025876239462 No Hit GATACCACTGCTTATGTACTCTGCG 157 0.1664987539105997 No Hit GTTGATACCACTGCTTATGTACTCT 153 0.16225674744153987 No Hit ATACCACTGCTTATGTACTCTGCGT 143 0.1516517312688902 No Hit GGTATCAACGCAGAGTACATGGGAA 141 0.14953072803436024 No Hit GAGTACATGGGAAGCAGTGGTATCA 141 0.14953072803436024 No Hit GTACTGGTTCACTATCGGTCAGTCA 140 0.14847022641709529 No Hit GAACATAAGCAGTGGTATCAACGCA 139 0.14740972479983033 No Hit GCAGTGGTATCAACGCAGAGTACAT 137 0.14528872156530037 No Hit CAGTGGTATCAACGCAGAGTACATA 128 0.13574420700991569 No Hit TATCAACGCAGAAAAAAAAAAAAAA 127 0.1346837053926507 No Hit GGTATCAACGCAGAGTACATGGGGA 124 0.13150220054085582 No Hit ATACAGGGTGACAGCCCCGTACACA 122 0.12938119730632588 No Hit GTATCAACGCAGAGTACATGGGGGG 121 0.12832069568906093 No Hit GTACACAAGCAGTGGTATCAACGCA 120 0.12726019407179595 No Hit GAATCAACGCAGAGTACTTTTTTTT 118 0.12513919083726602 No Hit GTATCAACGCAGAGTACATGGGGGT 117 0.12407868922000105 No Hit GTATCAACGCAGAGTACATGGGAAG 114 0.12089718436820616 No Hit GNATCAACGCAGAGTACTTTTTTTT 113 0.11983668275094118 No Hit TGATACCACTGCTTATGTACTCTGC 113 0.11983668275094118 No Hit GGATAAGCAGTGGTATCAACGCAGA 111 0.11771567951641126 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 110 0.1166551778991463 No Hit GGATCAACGCAGAGTACTTTTTTTT 110 0.1166551778991463 No Hit GCAGAGTACATAAGCAGTGGTATCA 104 0.1102921681955565 No Hit GCTTATCCCATGTACTCTGCGTTGA 104 0.1102921681955565 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 102 0.10817116496102658 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 102 0.10817116496102658 No Hit TACCACTGCTTATGTACTCTGCGTT 101 0.1071106633437616 No Hit GNTATCAACGCAGAGTACTTTTTTT 97 0.10286865687470173 No Hit GATTATGTACTCTGCGTTGATACCA 97 0.10286865687470173 No Hit GCGTTGATACCACTGCTTCCCATGT 95 0.1007476536401718 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATCAA 50 0.0011304222 13.784318 1 GATCAAC 50 0.0011304222 13.784318 2 GTACATA 705 0.0 11.870994 1 TACATAA 695 0.0 11.758463 2 ACATAAG 750 0.0 11.477426 3 AATCAAC 80 2.6552065E-4 11.076684 2 CATAAGC 800 0.0 10.760087 4 GAATCAA 85 4.6512912E-4 10.425114 1 TAAGCAG 885 0.0 9.833518 6 ATAAGCA 890 0.0 9.778274 5 GGAAGCA 80 0.0046093394 9.459417 8 AGCAGTG 945 0.0 9.309268 8 GCAGTGG 980 0.0 9.266368 9 CAGTGGT 980 0.0 9.266368 10 AGTGGTA 990 0.0 9.172768 11 AAGCAGT 975 0.0 9.022828 7 GGGAAGC 85 0.0075892955 8.90298 7 TGGTATC 1040 0.0 8.731769 13 GTGGTAT 1080 0.0 8.40837 12 GCTTATG 410 0.0 8.405072 1 >>END_MODULE