FastQCFastQC Report
Thu 26 May 2016
SRR1511987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94295
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA40254.26851900949149No Hit
GTATCAACGCAGAGTACTTTTTTTT27082.8718383795535285No Hit
GGTATCAACGCAGAGTACTTTTTTT19812.1008537038018984No Hit
GCTTATGTACTCTGCGTTGATACCA19772.0966116973328384No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT18771.9905615356063415No Hit
TATCAACGCAGAGTACTTTTTTTTT18171.926931438570444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA16911.7933082347950582No Hit
CTTATGTACTCTGCGTTGATACCAC13441.4253141736041146No Hit
GAGTACATAAGCAGTGGTATCAACG10441.1071636884246248No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9771.0361100800678722No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9140.9692984781801792No Hit
TATGTACTCTGCGTTGATACCACTG8770.9300599183413755No Hit
CATAAGCAGTGGTATCAACGCAGAG7230.7667426692825706No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6150.6522084946179543No Hit
GCGTTGATACCACTGCTTATGTACT5970.6331194655071849No Hit
CCCATGTACTCTGCGTTGATACCAC5500.5832758894957315No Hit
ACGCAGAGTACATAAGCAGTGGTAT5370.569489368471287No Hit
GGTATCAACGCAGAAAAAAAAAAAA5310.5631263587676971No Hit
GTATCAACGCAGAGTACATAAGCAG5230.5546423458295774No Hit
TATCAACGCAGAGTACATAAGCAGT4950.5249483005461584No Hit
CTGCTTATGTACTCTGCGTTGATAC4540.48146773423829475No Hit
GGTATCAACGCAGAGTACATAAGCA4340.4602577018929954No Hit
GGTATCAACGCAGAGAAAAAAAAAA4290.45495519380667054No Hit
ACTCTGCGTTGATACCACTGCTTAT3880.4114746274988069No Hit
GTACATGGGAAGCAGTGGTATCAAC3870.410414125881542No Hit
AAGCAGTGGTATCAACGCAGAGTAC3610.38284108383265286No Hit
GTGGTATCAACGCAGAGTACATGGG3580.37965957898085795No Hit
GCAGAGTACTTTTTTTTTTTTTTTT3430.36375205472188343No Hit
ACATAAGCAGTGGTATCAACGCAGA3210.3404210191420542No Hit
GTATCAACGCAGAGAAAAAAAAAAA3020.3202714884140198No Hit
GTGGTATCAACGCAGAGTACATAAG2840.30118245930325044No Hit
CATGTACTCTGCGTTGATACCACTG2820.2990614560687205No Hit
GTGGTATCAACGCAGAGTACTTTTT2710.2873959382788059No Hit
GTATCAACGCAGAAAAAAAAAAAAA2700.28633543666154093No Hit
ATCAACGCAGAGTACTTTTTTTTTT2660.28209343019248106No Hit
GGTATCAACGCAGAGTACATGGGGG2570.2725489156370963No Hit
GTACTCTGCGTTGATACCACTGCTT2220.23543135903282253No Hit
GCAGTGGTATCAACGCAGAGTACAT2020.2142213266875232No Hit
GTACATGGGATAAGCAGTGGTATCA1990.2110398218357283No Hit
CCACTGCTTATGTACTCTGCGTTGA1930.20467681213213848No Hit
GGTATCAACGCAGAGTACATGGGAA1840.19513229757675382No Hit
GATACCACTGCTTATGTACTCTGCG1800.19089029110769395No Hit
CAGTGGTATCAACGCAGAGTACATA1750.1855877830213691No Hit
GAGTACATGGGAAGCAGTGGTATCA1730.18346677978683917No Hit
GTACTGGTTCACTATCGGTCAGTCA1680.17816427170051433No Hit
TATCAACGCAGAGAAAAAAAAAAAA1670.17710377008324937No Hit
GTACACAAGCAGTGGTATCAACGCA1560.16543825229333475No Hit
GTTGATACCACTGCTTATGTACTCT1560.16543825229333475No Hit
ATACCACTGCTTATGTACTCTGCGT1490.15801474097247997No Hit
GGTATCAACGCAGAGTACATGGGGA1480.15695423935521502No Hit
GGATAAGCAGTGGTATCAACGCAGA1460.15483323612068509No Hit
GTATCAACGCAGAGTACATGGGAAG1390.14740972479983033No Hit
TGATACCACTGCTTATGTACTCTGC1360.14422821994803542No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1340.14210721671350549No Hit
ATACAGGGTGACAGCCCCGTACACA1330.14104671509624053No Hit
GTATCAACGCAGAGTACATGGGGGT1250.1325627021581208No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1220.12938119730632588No Hit
TACCACTGCTTATGTACTCTGCGTT1220.12938119730632588No Hit
GTATCAACGCAGAGTACATGGGGGG1210.12832069568906093No Hit
GAGTACATGGGATAAGCAGTGGTAT1190.126199692454531No Hit
GCTTATCCCATGTACTCTGCGTTGA1190.126199692454531No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1190.126199692454531No Hit
TATCAACGCAGAAAAAAAAAAAAAA1180.12513919083726602No Hit
GGGATAAGCAGTGGTATCAACGCAG1180.12513919083726602No Hit
ATCAACGCAGAGTACATAAGCAGTG1140.12089718436820616No Hit
TATCAACGCAGAGTACATGGGAAGC1130.11983668275094118No Hit
GGATACCACGTGTCCCGCCCTACTC1070.1134736730473514No Hit
GCAGAGTACATAAGCAGTGGTATCA1030.10923166657829154No Hit
GCGTTGATACCACTGCTTCCCATGT1030.10923166657829154No Hit
CAACGCAGAGTACTTTTTTTTTTTT1020.10817116496102658No Hit
TCAACGCAGAGTACTTTTTTTTTTT980.10392915849196671No Hit
GTACATGGGGGTTAAGCGACTAAGC980.10392915849196671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAGCA954.7439244E-913.986718
GGGAAGC957.1915565E-812.98765857
GAAGCAG1103.695277E-812.0794319
CAGAAAA550.003053489612.07943111
TGGGAAG1201.2091914E-711.0904636
GCAGAAA600.005847763311.07281210
TACATAA8300.010.5481042
GTACATA8350.010.518511
ACATAAG9000.09.621963
GCTTATG3950.09.1841591
CATAAGC9450.09.1637724
TAAGCAG10000.08.8406836
ATGGGAA1909.248106E-88.5145165
ATAAGCA10300.08.4999285
AGCAGTG10950.08.2341848
CATGGGT1304.00272E-48.0521894
GCAGTGG11350.07.9439939
AGTGGTA11300.07.89515311
CAGTGGT11400.07.825896710
AAGCAGT11650.07.82089477
Produced by FastQC (version 0.11.5)