##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511987_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94295 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.834710217933083 33.0 31.0 34.0 30.0 34.0 2 31.939986213478974 34.0 31.0 34.0 30.0 34.0 3 31.991855347579406 34.0 31.0 34.0 30.0 34.0 4 35.532371811867016 37.0 35.0 37.0 33.0 37.0 5 35.314131184050055 37.0 35.0 37.0 33.0 37.0 6 35.380974600986264 37.0 35.0 37.0 33.0 37.0 7 35.28099050851053 37.0 35.0 37.0 33.0 37.0 8 35.25620658571504 37.0 35.0 37.0 33.0 37.0 9 36.85119041306538 39.0 37.0 39.0 33.0 39.0 10 36.794909592237126 39.0 37.0 39.0 33.0 39.0 11 36.77367834985948 39.0 37.0 39.0 33.0 39.0 12 36.694628559308555 39.0 37.0 39.0 33.0 39.0 13 36.513972108807465 39.0 37.0 39.0 32.0 39.0 14 37.670141576965904 40.0 38.0 41.0 32.0 41.0 15 37.526613288085265 40.0 38.0 41.0 32.0 41.0 16 37.302762606712975 40.0 37.0 41.0 32.0 41.0 17 37.509486186966434 40.0 38.0 41.0 32.0 41.0 18 37.487565618537566 40.0 37.0 41.0 32.0 41.0 19 37.563380879155844 40.0 38.0 41.0 33.0 41.0 20 37.59850469271966 40.0 38.0 41.0 33.0 41.0 21 37.52308181769977 40.0 37.0 41.0 33.0 41.0 22 37.45824274882019 40.0 37.0 41.0 33.0 41.0 23 37.41846333315658 40.0 37.0 41.0 33.0 41.0 24 37.35602099793202 40.0 37.0 41.0 33.0 41.0 25 37.02361737101649 40.0 37.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05607190581931576 1101 2 -0.05022700105829969 1101 3 0.20662681905893976 1101 4 -0.1832731392522362 1101 5 -0.44209637886370245 1101 6 -0.08250401703224952 1101 7 -0.5310917190677031 1101 8 -0.49880738165875016 1101 9 -0.1632325252784952 1101 10 -0.7127037601582202 1101 11 -0.8047609674651 1101 12 -0.45390508451674805 1101 13 -0.6724753590152943 1101 14 -0.5817204962391926 1101 15 -1.144130180586565 1101 16 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0.45539676885683633 1104 14 0.30272838820969383 1104 15 0.35487081841444024 1104 16 -0.17660245133545516 1104 17 -0.2897227274120908 1104 18 0.042764616365076336 1104 19 0.29869264866395895 1104 20 0.3664813744664954 1104 21 0.5543135892385394 1104 22 0.5571492612360274 1104 23 0.26010724965634324 1104 24 0.04015976108592412 1104 25 -0.34128379391948016 1105 1 0.3628261272076827 1105 2 0.4407048313369515 1105 3 0.024638076094582573 1105 4 0.211973890141131 1105 5 0.0994921139443008 1105 6 0.33900942887144225 1105 7 0.10149114522247515 1105 8 -0.14671232224662134 1105 9 0.13101387872401205 1105 10 0.037765282993753146 1105 11 0.060467300202191154 1105 12 -0.06459926838166297 1105 13 -0.11619018077889365 1105 14 0.38888613290401963 1105 15 0.6888904573121266 1105 16 0.7643224773943516 1105 17 -0.21037524562558474 1105 18 0.15188720109741638 1105 19 0.26289917384609396 1105 20 -0.4895528352262559 1105 21 -0.5063452641032455 1105 22 -0.5939435483933622 1105 23 -0.7394176157221821 1105 24 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1.3771863669874236 1108 23 1.233205579897529 1108 24 1.2757812824217325 1108 25 1.4973061875276414 1109 1 0.10443443437986843 1109 2 -0.005926417153752084 1109 3 -0.25186518398752256 1109 4 0.2410731415296894 1109 5 0.29413109003703397 1109 6 0.07337310914190454 1109 7 0.2809164048216033 1109 8 0.006270049161251734 1109 9 0.17822469736685065 1109 10 -0.3033359002953091 1109 11 0.05370564842726111 1109 12 -0.3766012002821668 1109 13 -0.46912425467409236 1109 14 -0.020918262169629998 1109 15 -0.3493853214852649 1109 16 -0.6424633231369228 1109 17 -0.1725824419549724 1109 18 -0.676535885113644 1109 19 -0.4407955858737793 1109 20 0.20870845431733898 1109 21 -0.32365992239834185 1109 22 -0.1643371731216945 1109 23 0.16096876437924834 1109 24 -0.004073233642117202 1109 25 0.08368727461594005 1110 1 -0.16260941286200037 1110 2 0.01387556304426596 1110 3 -0.05271384028172221 1110 4 -0.028328084307183587 1110 5 -0.21572275249007333 1110 6 0.38709305256482196 1110 7 -0.24647633445231776 1110 8 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0.4092406372607229 2314 20 0.807195132253419 2314 21 0.538370183051228 2314 22 0.6255686467285884 2314 23 1.0695254737087154 2314 24 0.9109231440444319 2314 25 0.946119859337827 2315 1 -0.6220019887900108 2315 2 -0.5924236495541599 2315 3 -0.7170557975370642 2315 4 -0.7145673044046674 2315 5 -0.5363826700003855 2315 6 -0.3233681397861332 2315 7 -0.02180441844182468 2315 8 -0.5013311046549163 2315 9 -0.49535447157366974 2315 10 -0.8359623912908631 2315 11 -0.7152192755612035 2315 12 -0.620773649023107 2315 13 -0.19579455949985203 2315 14 -0.503182236598569 2315 15 -0.5987031666556106 2315 16 -0.7155123603241051 2315 17 -0.6592901678456684 2315 18 -0.2999084585914602 2315 19 -0.39698175567186667 2315 20 -0.7354717904787975 2315 21 -0.6798616843068146 2315 22 -0.47352872219785525 2315 23 -0.42973833782703963 2315 24 -0.3848325694182151 2315 25 0.015705673306207757 2316 1 -0.4199593093423317 2316 2 -0.6890229876803708 2316 3 -0.6328380638842077 2316 4 -0.8488249787171185 2316 5 -0.7598221313720614 2316 6 -0.2168406948471855 2316 7 -0.08125894314797222 2316 8 -0.1721630115361208 2316 9 -0.3739348664156168 2316 10 -0.6034506943834117 2316 11 -0.6581611904305404 2316 12 -0.30284599867505335 2316 13 -0.33746978487928914 2316 14 -0.27848749735402123 2316 15 0.026995794887241686 2316 16 -0.49760753321010043 2316 17 -0.5884240261133868 2316 18 -0.2560307911259869 2316 19 -0.5216536716823654 2316 20 -0.27392893985686584 2316 21 -0.2709263881753543 2316 22 -0.15845397610633682 2316 23 0.040363225564497895 2316 24 -0.05903090332213168 2316 25 -0.0510522862966738 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 827.0 3 0.0 4 15.0 5 140.0 6 175.0 7 142.0 8 134.0 9 91.0 10 86.0 11 83.0 12 79.0 13 74.0 14 71.0 15 76.0 16 77.0 17 89.0 18 82.0 19 88.0 20 125.0 21 157.0 22 192.0 23 268.0 24 323.0 25 382.0 26 419.0 27 533.0 28 588.0 29 758.0 30 988.0 31 1388.0 32 1735.0 33 2453.0 34 3316.0 35 4832.0 36 9300.0 37 16341.0 38 47868.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.929138473014014 18.351537109062814 15.075923064627844 16.643401353295328 2 14.16618060342542 24.160347844530463 40.4751047245347 21.198366827509414 3 19.03918553475794 32.79601251391908 33.25520971419482 14.90959223712816 4 11.002704279124027 20.692507556074023 35.49923113632748 32.80555702847447 5 8.895513520968375 43.79223644985731 33.0815501639066 14.230699865267715 6 24.90610478069896 34.66271988456723 23.644619856982196 16.78655547775161 7 20.433745161461374 40.733867119147355 21.26623893101437 17.566148788376903 8 23.279071000583276 34.01028686568747 24.16777135585132 18.542870777877937 9 29.275147144599394 17.702953497004085 19.510048252823587 33.51185110557294 10 17.5873588207222 27.393817275571347 27.893313537303143 27.12551036640331 11 27.01521819820775 28.796860915212896 21.585449917811125 22.602470968768227 12 27.051275253194763 29.34195874648709 26.06394824752108 17.542817752797074 13 29.101224879367944 23.110451243438145 32.03351185110557 15.754812026088338 14 27.958004135956305 26.129699347791508 27.43093483217562 18.481361684076568 15 28.881701044594095 25.327960125139192 28.743835834349646 17.046502995917066 16 20.069993106739485 28.916697597963836 32.13107799989395 18.882231295402725 17 16.528978206691765 33.87772416352935 29.476642451879737 20.116655177899144 18 15.814200116655178 25.74579776234159 41.070046131820355 17.369955989182884 19 18.61816639270375 31.23389363168779 32.10456545946233 18.043374516146137 20 18.722095551195718 29.349382257807942 35.82692613606236 16.101596054933985 21 20.265125404316244 30.83726602683069 32.091839440055146 16.80576912879792 22 18.020043480566308 29.306962193117343 30.962405217667953 21.71058910864839 23 21.715891616734716 25.91017551301766 32.461954504480616 19.91197836576701 24 16.625660169257852 29.042144781216194 30.80047510976308 23.53171993976287 25 19.35237529565245 31.460602653712762 32.16168344240213 17.025338608232662 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3608.0 1 3608.0 2 2062.5 3 517.0 4 517.0 5 517.0 6 859.5 7 1202.0 8 1202.0 9 1202.0 10 1009.5 11 817.0 12 817.0 13 817.0 14 654.0 15 491.0 16 491.0 17 491.0 18 1018.5 19 1546.0 20 1546.0 21 1546.0 22 2393.0 23 3240.0 24 3240.0 25 3240.0 26 4498.5 27 5757.0 28 5757.0 29 5757.0 30 6183.5 31 6610.0 32 6610.0 33 6610.0 34 6439.5 35 6269.0 36 6269.0 37 6269.0 38 6279.5 39 6290.0 40 6290.0 41 6290.0 42 13437.0 43 20584.0 44 20584.0 45 20584.0 46 16744.5 47 12905.0 48 12905.0 49 12905.0 50 11171.0 51 9437.0 52 9437.0 53 9437.0 54 7832.5 55 6228.0 56 6228.0 57 6228.0 58 5018.0 59 3808.0 60 3808.0 61 3808.0 62 3221.0 63 2634.0 64 2634.0 65 2634.0 66 1989.0 67 1344.0 68 1344.0 69 1344.0 70 1009.0 71 674.0 72 674.0 73 674.0 74 444.5 75 215.0 76 215.0 77 215.0 78 148.0 79 81.0 80 81.0 81 81.0 82 54.5 83 28.0 84 28.0 85 28.0 86 17.5 87 7.0 88 7.0 89 7.0 90 4.0 91 1.0 92 1.0 93 1.0 94 1.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47722572776923483 2 0.0 3 0.0 4 0.0 5 0.036057054987008855 6 0.04348056630786362 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0010605016172649663 25 0.012726019407179595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 94295.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.97110133092953 #Duplication Level Percentage of deduplicated Percentage of total 1 76.28578360019408 26.67797868391749 2 13.361232411450752 9.345140251338883 3 4.482047549733139 4.70226417095286 4 1.8225376031052887 2.549445887904979 5 0.9613051916545367 1.6808950633649715 6 0.5215914604560893 1.0944376690174453 7 0.39725861232411447 0.9724799830319741 8 0.309315866084425 0.8653693196882126 9 0.18801552644347405 0.5917599024338512 >10 1.282751091703057 9.102285380985206 >50 0.17588549247937896 4.139137812185163 >100 0.1546579330422125 11.439630945437191 >500 0.030325084910237745 7.25807306856143 >1k 0.02729257641921397 19.58110186118034 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATAAGCAGTGGTATCAACGCA 4025 4.26851900949149 No Hit GTATCAACGCAGAGTACTTTTTTTT 2708 2.8718383795535285 No Hit GGTATCAACGCAGAGTACTTTTTTT 1981 2.1008537038018984 No Hit GCTTATGTACTCTGCGTTGATACCA 1977 2.0966116973328384 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 1877 1.9905615356063415 No Hit TATCAACGCAGAGTACTTTTTTTTT 1817 1.926931438570444 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1691 1.7933082347950582 No Hit CTTATGTACTCTGCGTTGATACCAC 1344 1.4253141736041146 No Hit GAGTACATAAGCAGTGGTATCAACG 1044 1.1071636884246248 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 977 1.0361100800678722 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 914 0.9692984781801792 No Hit TATGTACTCTGCGTTGATACCACTG 877 0.9300599183413755 No Hit CATAAGCAGTGGTATCAACGCAGAG 723 0.7667426692825706 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 615 0.6522084946179543 No Hit GCGTTGATACCACTGCTTATGTACT 597 0.6331194655071849 No Hit CCCATGTACTCTGCGTTGATACCAC 550 0.5832758894957315 No Hit ACGCAGAGTACATAAGCAGTGGTAT 537 0.569489368471287 No Hit GGTATCAACGCAGAAAAAAAAAAAA 531 0.5631263587676971 No Hit GTATCAACGCAGAGTACATAAGCAG 523 0.5546423458295774 No Hit TATCAACGCAGAGTACATAAGCAGT 495 0.5249483005461584 No Hit CTGCTTATGTACTCTGCGTTGATAC 454 0.48146773423829475 No Hit GGTATCAACGCAGAGTACATAAGCA 434 0.4602577018929954 No Hit GGTATCAACGCAGAGAAAAAAAAAA 429 0.45495519380667054 No Hit ACTCTGCGTTGATACCACTGCTTAT 388 0.4114746274988069 No Hit GTACATGGGAAGCAGTGGTATCAAC 387 0.410414125881542 No Hit AAGCAGTGGTATCAACGCAGAGTAC 361 0.38284108383265286 No Hit GTGGTATCAACGCAGAGTACATGGG 358 0.37965957898085795 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 343 0.36375205472188343 No Hit ACATAAGCAGTGGTATCAACGCAGA 321 0.3404210191420542 No Hit GTATCAACGCAGAGAAAAAAAAAAA 302 0.3202714884140198 No Hit GTGGTATCAACGCAGAGTACATAAG 284 0.30118245930325044 No Hit CATGTACTCTGCGTTGATACCACTG 282 0.2990614560687205 No Hit GTGGTATCAACGCAGAGTACTTTTT 271 0.2873959382788059 No Hit GTATCAACGCAGAAAAAAAAAAAAA 270 0.28633543666154093 No Hit ATCAACGCAGAGTACTTTTTTTTTT 266 0.28209343019248106 No Hit GGTATCAACGCAGAGTACATGGGGG 257 0.2725489156370963 No Hit GTACTCTGCGTTGATACCACTGCTT 222 0.23543135903282253 No Hit GCAGTGGTATCAACGCAGAGTACAT 202 0.2142213266875232 No Hit GTACATGGGATAAGCAGTGGTATCA 199 0.2110398218357283 No Hit CCACTGCTTATGTACTCTGCGTTGA 193 0.20467681213213848 No Hit GGTATCAACGCAGAGTACATGGGAA 184 0.19513229757675382 No Hit GATACCACTGCTTATGTACTCTGCG 180 0.19089029110769395 No Hit CAGTGGTATCAACGCAGAGTACATA 175 0.1855877830213691 No Hit GAGTACATGGGAAGCAGTGGTATCA 173 0.18346677978683917 No Hit GTACTGGTTCACTATCGGTCAGTCA 168 0.17816427170051433 No Hit TATCAACGCAGAGAAAAAAAAAAAA 167 0.17710377008324937 No Hit GTACACAAGCAGTGGTATCAACGCA 156 0.16543825229333475 No Hit GTTGATACCACTGCTTATGTACTCT 156 0.16543825229333475 No Hit ATACCACTGCTTATGTACTCTGCGT 149 0.15801474097247997 No Hit GGTATCAACGCAGAGTACATGGGGA 148 0.15695423935521502 No Hit GGATAAGCAGTGGTATCAACGCAGA 146 0.15483323612068509 No Hit GTATCAACGCAGAGTACATGGGAAG 139 0.14740972479983033 No Hit TGATACCACTGCTTATGTACTCTGC 136 0.14422821994803542 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 134 0.14210721671350549 No Hit ATACAGGGTGACAGCCCCGTACACA 133 0.14104671509624053 No Hit GTATCAACGCAGAGTACATGGGGGT 125 0.1325627021581208 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 122 0.12938119730632588 No Hit TACCACTGCTTATGTACTCTGCGTT 122 0.12938119730632588 No Hit GTATCAACGCAGAGTACATGGGGGG 121 0.12832069568906093 No Hit GAGTACATGGGATAAGCAGTGGTAT 119 0.126199692454531 No Hit GCTTATCCCATGTACTCTGCGTTGA 119 0.126199692454531 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 119 0.126199692454531 No Hit TATCAACGCAGAAAAAAAAAAAAAA 118 0.12513919083726602 No Hit GGGATAAGCAGTGGTATCAACGCAG 118 0.12513919083726602 No Hit ATCAACGCAGAGTACATAAGCAGTG 114 0.12089718436820616 No Hit TATCAACGCAGAGTACATGGGAAGC 113 0.11983668275094118 No Hit GGATACCACGTGTCCCGCCCTACTC 107 0.1134736730473514 No Hit GCAGAGTACATAAGCAGTGGTATCA 103 0.10923166657829154 No Hit GCGTTGATACCACTGCTTCCCATGT 103 0.10923166657829154 No Hit CAACGCAGAGTACTTTTTTTTTTTT 102 0.10817116496102658 No Hit TCAACGCAGAGTACTTTTTTTTTTT 98 0.10392915849196671 No Hit GTACATGGGGGTTAAGCGACTAAGC 98 0.10392915849196671 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0010605016172649663 0.0 10 0.0 0.0 0.0 0.0010605016172649663 0.0 11 0.0 0.0 0.0 0.0010605016172649663 0.0 12 0.0 0.0 0.0 0.0010605016172649663 0.0 13 0.0 0.0 0.0 0.0010605016172649663 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAAGCA 95 4.7439244E-9 13.98671 8 GGGAAGC 95 7.1915565E-8 12.9876585 7 GAAGCAG 110 3.695277E-8 12.079431 9 CAGAAAA 55 0.0030534896 12.079431 11 TGGGAAG 120 1.2091914E-7 11.090463 6 GCAGAAA 60 0.0058477633 11.072812 10 TACATAA 830 0.0 10.548104 2 GTACATA 835 0.0 10.51851 1 ACATAAG 900 0.0 9.62196 3 GCTTATG 395 0.0 9.184159 1 CATAAGC 945 0.0 9.163772 4 TAAGCAG 1000 0.0 8.840683 6 ATGGGAA 190 9.248106E-8 8.514516 5 ATAAGCA 1030 0.0 8.499928 5 AGCAGTG 1095 0.0 8.234184 8 CATGGGT 130 4.00272E-4 8.052189 4 GCAGTGG 1135 0.0 7.943993 9 AGTGGTA 1130 0.0 7.895153 11 CAGTGGT 1140 0.0 7.8258967 10 AAGCAGT 1165 0.0 7.8208947 7 >>END_MODULE