FastQCFastQC Report
Thu 26 May 2016
SRR1511986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347690
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28960.2148862127047021No Hit
GGTATCAACGCAGAGTACTTTTTTT17390.1290356090792393No Hit
TATCAACGCAGAGTACTTTTTTTTT16840.12495455186281712No Hit
CTGTAGGACGTGGAATATGGCAAGA15740.11679243742997276No Hit
CTTTAGGACGTGAAATATGGCGAGG15630.11597622598668834No Hit
GTCCTACAGTGGACATTTCTAAATT15550.11538261766429966No Hit
GTCCTAAAGTGTGTATTTCTCATTT14590.1082593177956355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCT604.2156302E-412.6218239
ACCGTGC604.2156302E-412.6218238
TGTCGAG854.096526E-612.25195718
GGCGAGG4900.011.97918619
GATATAC2400.011.4251751
GTCTTAG1406.2032996E-810.4925081
GGTATCA8950.010.3948121
GCCTAGG1157.1456634E-610.218791
AGGACGT10600.010.180815
GACGTGA6600.09.8966577
GGACGTG10450.09.874016
TGGCGAG10550.09.87124418
CCTTATA1302.8749328E-69.7930072
GTATTAA2209.094947E-129.7930071
TAGGACG11650.09.5069964
TAGACTG1701.7613274E-89.4663685
ATTAGAC1107.103112E-59.4663673
TTAAACC1304.430791E-69.4663673
TAGGCCC900.0011500089.4663674
CTTAGAC1304.430791E-69.4663673
Produced by FastQC (version 0.11.5)