##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511986_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1347690 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.209310004526262 31.0 30.0 34.0 25.0 34.0 2 27.239663424081204 31.0 26.0 34.0 10.0 34.0 3 29.228117742210745 31.0 28.0 34.0 16.0 34.0 4 33.80494178928389 35.0 33.0 37.0 28.0 37.0 5 34.27491188626465 37.0 35.0 37.0 32.0 37.0 6 34.57774933404566 37.0 35.0 37.0 32.0 37.0 7 34.67187261165402 37.0 35.0 37.0 32.0 37.0 8 34.72016413270114 37.0 35.0 37.0 32.0 37.0 9 36.424543478099565 39.0 37.0 39.0 32.0 39.0 10 36.35440865480934 39.0 37.0 39.0 32.0 39.0 11 36.36601814957446 39.0 37.0 39.0 32.0 39.0 12 36.291046902477575 39.0 37.0 39.0 32.0 39.0 13 36.285662132983106 39.0 37.0 39.0 32.0 39.0 14 37.58398889952437 40.0 38.0 41.0 32.0 41.0 15 37.525684690099354 40.0 38.0 41.0 32.0 41.0 16 37.48384198146458 40.0 38.0 41.0 32.0 41.0 17 37.45873309143794 40.0 38.0 41.0 32.0 41.0 18 37.41626561004385 40.0 38.0 41.0 32.0 41.0 19 37.45029346511438 40.0 38.0 41.0 32.0 41.0 20 37.36992335032537 40.0 38.0 41.0 32.0 41.0 21 37.32320563334298 40.0 38.0 41.0 32.0 41.0 22 37.23848511156127 40.0 38.0 41.0 32.0 41.0 23 37.155219672179804 40.0 38.0 41.0 32.0 41.0 24 37.08619489645245 40.0 38.0 41.0 32.0 41.0 25 36.99343394994398 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2197177947386884 1101 2 4.069362023939931 1101 3 2.3915123735067354 1101 4 1.295656103618029 1101 5 0.9344340069485639 1101 6 0.6877514284099888 1101 7 0.301692425831142 1101 8 0.10939076163476358 1101 9 0.21263458230529153 1101 10 0.34370481130928 1101 11 0.4428484705471689 1101 12 0.35375918191896005 1101 13 0.32566837135810545 1101 14 0.1953306763980649 1101 15 0.2559807507822285 1101 16 0.3708270459774994 1101 17 0.448561372740528 1101 18 0.666600738281403 1101 19 0.6126853595307153 1101 20 0.4643530417560129 1101 21 0.5650155127858341 1101 22 0.5719076697763725 1101 23 0.4822490389903962 1101 24 0.32398125651055665 1101 25 0.2576831766415282 1102 1 1.5331448875849603 1102 2 4.340524276603553 1102 3 2.5902374876619874 1102 4 1.682936468914832 1102 5 1.1315018912712418 1102 6 0.9840314892927893 1102 7 0.8970555917367733 1102 8 0.8747849777687051 1102 9 0.8966224057451697 1102 10 0.6881979619942129 1102 11 0.7924034172747199 1102 12 0.8176313280376775 1102 13 0.7211045966244711 1102 14 0.7506560949673187 1102 15 0.7519454387578719 1102 16 0.7683710338009391 1102 17 0.8178922707618383 1102 18 1.0049179376725732 1102 19 0.8880497430923526 1102 20 0.9044346247088271 1102 21 0.7884980089806604 1102 22 0.8491253257885489 1102 23 0.8777266645611732 1102 24 0.9034540114572849 1102 25 0.775694744373645 1103 1 1.3557685500256973 1103 2 4.273557794332678 1103 3 2.4481262707877036 1103 4 1.6366397894186306 1103 5 1.2453065145014364 1103 6 1.0634251444220695 1103 7 0.7717492234142256 1103 8 0.6941702178281162 1103 9 0.628918570220705 1103 10 0.7058075303425184 1103 11 0.7291650868613715 1103 12 0.6959404508011318 1103 13 0.7616889151647541 1103 14 0.8204678365189082 1103 15 1.0578550710239227 1103 16 0.778323118485055 1103 17 0.8918943032541193 1103 18 0.9668581400940894 1103 19 0.9155711601349381 1103 20 0.9845306852605376 1103 21 0.9952995007012433 1103 22 0.964675041377582 1103 23 1.0291082534919056 1103 24 1.0892948516767191 1103 25 1.0538426932578417 1104 1 1.3607626926978718 1104 2 3.8213275568197496 1104 3 2.170555155063802 1104 4 1.389083768017123 1104 5 1.1557721777724481 1104 6 0.7948443989315308 1104 7 0.5090623426867751 1104 8 0.6536810110678744 1104 9 0.5254236979515525 1104 10 0.4330079103266655 1104 11 0.36190092708533683 1104 12 0.6195913814654403 1104 13 0.489630880806331 1104 14 0.5343202455590585 1104 15 0.4407753086053603 1104 16 0.5948705833924635 1104 17 0.6314076312439312 1104 18 0.6141756437991646 1104 19 0.7881211871951095 1104 20 0.6356080682110345 1104 21 0.7982672134661044 1104 22 0.661739869322858 1104 23 0.7677389860803459 1104 24 0.6805121710986128 1104 25 0.7114778856362349 1105 1 1.5084588845766866 1105 2 4.325266589477486 1105 3 2.50460928072323 1105 4 1.565627237638644 1105 5 1.112341517994217 1105 6 0.8515688069077783 1105 7 0.7203363139018393 1105 8 0.5780328824005991 1105 9 0.630690546959201 1105 10 0.5313395683048654 1105 11 0.4399724764381858 1105 12 0.5813114646877366 1105 13 0.5619205068514788 1105 14 0.534378688180098 1105 15 0.4926723560841424 1105 16 0.5883834524576201 1105 17 0.7801207247287465 1105 18 0.4332528737747765 1105 19 0.6283507498105365 1105 20 0.5369880933023978 1105 21 0.621887383183477 1105 22 0.6109415431195231 1105 23 0.5364075073261816 1105 24 0.6510492977476687 1105 25 0.6733226464500675 1106 1 0.5671018655351503 1106 2 2.5409761832319653 1106 3 1.3406415770465578 1106 4 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0.36033225702763616 1107 16 0.4823532766902119 1107 17 0.5018010494488294 1107 18 0.49526436796546136 1107 19 0.4187332654822171 1107 20 0.5334733944378627 1107 21 0.7491036832487268 1107 22 0.6024264059997364 1107 23 0.683250067645794 1107 24 0.7603609773040887 1107 25 0.7460540803887739 1108 1 1.2453375827245559 1108 2 3.598897006272086 1108 3 2.176479157959033 1108 4 1.3991924993777474 1108 5 0.9373965511181765 1108 6 0.734974043250979 1108 7 0.5192132738876793 1108 8 0.5808253906100305 1108 9 0.5286063137540609 1108 10 0.6055705356909087 1108 11 0.513641403409359 1108 12 0.5672432808588894 1108 13 0.7064825056337227 1108 14 0.6128119484828716 1108 15 0.6727553090861136 1108 16 0.7538715689456978 1108 17 0.7751253303377013 1108 18 0.6725145192001278 1108 19 0.7106839461031527 1108 20 0.7483756565969983 1108 21 0.6167186929978499 1108 22 0.8009112033452794 1108 23 0.7386702404038203 1108 24 0.7321028112808747 1108 25 0.8095503144507106 1109 1 0.8279603034494905 1109 2 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-0.5973151893993176 2315 8 -0.6464248696277579 2315 9 -0.5188417035998469 2315 10 -0.6274561773346718 2315 11 -0.7397369669945846 2315 12 -0.6369261888225282 2315 13 -0.6542290234314763 2315 14 -0.6436774732345327 2315 15 -1.274410164381436 2315 16 -0.9949348778701932 2315 17 -0.8336040247210619 2315 18 -0.9064771508301561 2315 19 -0.8508801715314149 2315 20 -0.8243806923789236 2315 21 -0.6836898980358086 2315 22 -0.8198946014327078 2315 23 -0.8847617826528875 2315 24 -0.8360417227813599 2315 25 -0.809140069851459 2316 1 -0.49663455983194993 2316 2 0.03191825662690917 2316 3 0.08745475577820372 2316 4 -0.25948627865344065 2316 5 -0.6220956329406349 2316 6 -0.7863815355789328 2316 7 -0.95112530270071 2316 8 -1.0488585458444604 2316 9 -0.9930717888858425 2316 10 -1.209935077887394 2316 11 -1.2148390640788733 2316 12 -1.2727726740367231 2316 13 -1.185725534459067 2316 14 -1.2510061657071958 2316 15 -1.0525444569740046 2316 16 -1.174637773967099 2316 17 -1.3201112034367597 2316 18 -1.316470729862644 2316 19 -1.243045388391728 2316 20 -1.1366918335902483 2316 21 -1.273505262837432 2316 22 -1.346967676484013 2316 23 -1.1537276923115414 2316 24 -1.2013295468135397 2316 25 -1.122170562970659 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28397.0 3 0.0 4 216.0 5 1113.0 6 1293.0 7 1262.0 8 1121.0 9 1193.0 10 1072.0 11 1085.0 12 1124.0 13 1031.0 14 1096.0 15 1166.0 16 1216.0 17 1342.0 18 1542.0 19 1768.0 20 2372.0 21 3097.0 22 3810.0 23 4530.0 24 5287.0 25 6218.0 26 7104.0 27 8524.0 28 9973.0 29 12697.0 30 16325.0 31 21541.0 32 29550.0 33 41913.0 34 60947.0 35 94263.0 36 160221.0 37 228533.0 38 583748.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.88463222254376 19.216956421729034 14.04410509835348 24.85430625737373 2 16.748566937160156 21.690446145391018 38.86770381423453 22.6932831032143 3 17.861748621715677 25.180642432606902 30.517107049840842 26.440501895836583 4 12.517418694210091 17.644191171560227 38.55753177659551 31.280858357634173 5 13.470827861006612 37.5141167479168 35.627555298325284 13.387500092751301 6 30.688311620856147 37.32322516544989 18.810422879462546 13.178040334231413 7 27.202175574501553 32.02873064280361 22.830398682189525 17.93869510050531 8 25.04322210597393 36.160838174951216 21.17000200342809 17.62593771564677 9 26.106003606170557 16.011100475628666 20.820069897379962 37.06282602082081 10 15.424318648947457 28.762623451980794 34.00759818652658 21.80545971254517 11 32.92433719920753 23.061757525840513 25.303222551180166 18.71068272377179 12 23.19235135676602 24.778918000430366 31.45307897216719 20.57565167063642 13 27.95346110752473 22.261350904139675 26.495039660456037 23.290148327879557 14 21.385185020293985 22.1708256349754 27.79029301990814 28.653696324822477 15 22.796266203652173 30.188544843398706 24.005297954277317 23.0098909986718 16 22.223656775667994 28.18875260631154 27.487330172369017 22.100260445651447 17 21.637913763550966 28.52903857712085 27.877256639138082 21.9557910201901 18 21.513728417523758 27.59433069744353 29.580411952697933 21.311528932334777 19 23.211753575602426 27.150462834140278 27.873782625633037 21.764000964624262 20 23.39275405549397 27.317356223074057 28.134984766390787 21.15490495504119 21 23.041071148981512 26.810038608319598 27.45761655401269 22.6912736886862 22 23.194664577137463 27.54038074546329 27.521236621373745 21.7437180560255 23 22.599439775910366 27.429387649099375 27.75632107147495 22.214851503515312 24 22.995455921548608 26.901552881675876 28.02302906904269 22.079962127732827 25 22.845637404614138 27.216691746729943 27.85660436719587 22.081066481460045 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 333.0 1 333.0 2 359.5 3 386.0 4 386.0 5 386.0 6 866.0 7 1346.0 8 1346.0 9 1346.0 10 2029.5 11 2713.0 12 2713.0 13 2713.0 14 4609.5 15 6506.0 16 6506.0 17 6506.0 18 11640.0 19 16774.0 20 16774.0 21 16774.0 22 26092.0 23 35410.0 24 35410.0 25 35410.0 26 50315.5 27 65221.0 28 65221.0 29 65221.0 30 83952.0 31 102683.0 32 102683.0 33 102683.0 34 115657.5 35 128632.0 36 128632.0 37 128632.0 38 139888.5 39 151145.0 40 151145.0 41 151145.0 42 160631.0 43 170117.0 44 170117.0 45 170117.0 46 172007.0 47 173897.0 48 173897.0 49 173897.0 50 168557.5 51 163218.0 52 163218.0 53 163218.0 54 146305.0 55 129392.0 56 129392.0 57 129392.0 58 109990.0 59 90588.0 60 90588.0 61 90588.0 62 73990.5 63 57393.0 64 57393.0 65 57393.0 66 43885.5 67 30378.0 68 30378.0 69 30378.0 70 22028.0 71 13678.0 72 13678.0 73 13678.0 74 9392.5 75 5107.0 76 5107.0 77 5107.0 78 3510.0 79 1913.0 80 1913.0 81 1913.0 82 1249.0 83 585.0 84 585.0 85 585.0 86 384.5 87 184.0 88 184.0 89 184.0 90 127.5 91 71.0 92 71.0 93 71.0 94 43.0 95 15.0 96 15.0 97 15.0 98 10.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.330587894842286 3 0.0 4 0.0 5 0.0 6 7.420104029858499E-5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 8.162114432844348E-4 19 0.0011130156044787747 20 8.904124835830199E-4 21 0.003932655135825004 22 0.0013356187253745298 23 0.0011130156044787747 24 1.4840208059716998E-4 25 4.4520624179150996E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1347690.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.30520507360826 #Duplication Level Percentage of deduplicated Percentage of total 1 83.01383723688322 61.68360199833746 2 11.850532912305372 17.611125565607892 3 2.875041516055319 6.4089164833688415 4 1.0503160136340473 3.121757871406904 5 0.44968533307164815 1.6706980446241324 6 0.23500775376918023 1.0477379602624184 7 0.13232640259985406 0.6882778337284503 8 0.08290430435730167 0.49281770694033084 9 0.06153358504262088 0.41150390899556316 >10 0.2217065055046746 2.7884813283184857 >50 0.013183500430771988 0.6724768900641647 >100 0.012007771919292287 1.8869550365579677 >500 0.0012108406905747575 0.5806927719264454 >1k 7.063237361686085E-4 0.9349565998609135 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2896 0.2148862127047021 No Hit GGTATCAACGCAGAGTACTTTTTTT 1739 0.1290356090792393 No Hit TATCAACGCAGAGTACTTTTTTTTT 1684 0.12495455186281712 No Hit CTGTAGGACGTGGAATATGGCAAGA 1574 0.11679243742997276 No Hit CTTTAGGACGTGAAATATGGCGAGG 1563 0.11597622598668834 No Hit GTCCTACAGTGGACATTTCTAAATT 1555 0.11538261766429966 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1459 0.1082593177956355 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 7.420104029858499E-5 0.0 0.0 0.0 5 0.0 7.420104029858499E-5 0.0 0.0 0.0 6 0.0 7.420104029858499E-5 0.0 0.0 0.0 7 0.0 7.420104029858499E-5 0.0 0.0 0.0 8 0.0 7.420104029858499E-5 0.0 0.0 0.0 9 0.0 7.420104029858499E-5 0.0 0.0 0.0 10 0.0 7.420104029858499E-5 0.0 0.0 0.0 11 0.0 7.420104029858499E-5 0.0 0.0 0.0 12 0.0 7.420104029858499E-5 0.0 0.0 0.0 13 0.0 7.420104029858499E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTGCT 60 4.2156302E-4 12.621823 9 ACCGTGC 60 4.2156302E-4 12.621823 8 TGTCGAG 85 4.096526E-6 12.251957 18 GGCGAGG 490 0.0 11.979186 19 GATATAC 240 0.0 11.425175 1 GTCTTAG 140 6.2032996E-8 10.492508 1 GGTATCA 895 0.0 10.394812 1 GCCTAGG 115 7.1456634E-6 10.21879 1 AGGACGT 1060 0.0 10.18081 5 GACGTGA 660 0.0 9.896657 7 GGACGTG 1045 0.0 9.87401 6 TGGCGAG 1055 0.0 9.871244 18 CCTTATA 130 2.8749328E-6 9.793007 2 GTATTAA 220 9.094947E-12 9.793007 1 TAGGACG 1165 0.0 9.506996 4 TAGACTG 170 1.7613274E-8 9.466368 5 ATTAGAC 110 7.103112E-5 9.466367 3 TTAAACC 130 4.430791E-6 9.466367 3 TAGGCCC 90 0.001150008 9.466367 4 CTTAGAC 130 4.430791E-6 9.466367 3 >>END_MODULE