FastQCFastQC Report
Thu 26 May 2016
SRR1511986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347690
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35270.26170706913310926No Hit
TATCAACGCAGAGTACTTTTTTTTT21950.16287128345539406No Hit
GGTATCAACGCAGAGTACTTTTTTT21510.1596064376822563No Hit
CTGTAGGACGTGGAATATGGCAAGA16960.12584496434640013No Hit
CTTTAGGACGTGAAATATGGCGAGG16940.12569656226580295No Hit
GTCCTACAGTGGACATTTCTAAATT15800.11723764367176429No Hit
GTCCTAAAGTGTGTATTTCTCATTT14840.11011434380310012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1301.8189894E-1213.87642818
CCAACGA1251.4206307E-912.1537319
AGACAGC2200.011.2298186
AGCCGTG600.005900822611.07679757
TCTATAC1053.4412824E-610.86323453
ATAGCAC1701.364242E-1010.6236043
GGTATCA10600.010.5398861
TACACCG750.002634889410.1390195
TAGACAG1803.8926373E-1010.0334045
ATCCCGT951.653266E-49.994102510
CAGGACT2201.8189894E-129.9373914
TAAGTCT1151.0189971E-59.9186054
TATACAC3950.09.8662933
CTACACC1451.6022568E-79.83314
AGGACTG2350.09.7075725
AACAGTG3650.09.6244587
GATATAC3300.09.5489581
GGTTATA1409.85985E-79.5489571
CAGTACT1804.134563E-99.5053314
TAGGACA3300.09.505334
Produced by FastQC (version 0.11.5)