FastQCFastQC Report
Thu 26 May 2016
SRR1511985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032934
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37220.18308513704822685No Hit
GTCCTACAGTGGACATTTCTAAATT30190.14850457516082666No Hit
CTGTAGGACGTGGAATATGGCAAGA27550.1355184182073791No Hit
GTCCTAAAGTGTGTATTTCTCATTT26190.12882857977681517No Hit
CTTTAGGACGTGAAATATGGCGAGG25810.1269593602153341No Hit
TATCAACGCAGAGTACTTTTTTTTT22040.10841473456590328No Hit
GGTATCAACGCAGAGTACTTTTTTT21830.10738174480824268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1155.6243152E-912.34616618
TGGACCG550.003154609412.0465685
ACCGTTC658.2764355E-411.6494288
GGCCTAC1353.5695848E-810.8957771
GGCGAGG8250.010.67007919
TAGGACC5600.010.6483064
GTCCTAT1957.2759576E-1210.5605231
TGGCGAG16850.010.16757318
GGCGTGC856.8213075E-410.02193458
CCAACGA1252.693152E-69.8440119
AGGACGT17900.09.8353075
ATTTAGA6100.09.80621
TCTAGAC1651.0855729E-89.7519843
GGACGTG17500.09.7355946
ATTACCC900.00115187879.465163
GTAGAAC5800.09.465163
CTAGGAC2900.09.465163
GTCCTAC16650.09.3644781
TTAGACA2850.09.2991064
GTATCAA22200.09.2761351
Produced by FastQC (version 0.11.5)