##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511985_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2032934 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.487261268688506 31.0 30.0 34.0 25.0 34.0 2 26.543660541857236 31.0 25.0 34.0 10.0 34.0 3 28.5113235353435 31.0 28.0 34.0 16.0 34.0 4 33.012283232018355 35.0 33.0 37.0 27.0 37.0 5 33.472488039454305 35.0 35.0 37.0 28.0 37.0 6 33.75432945683431 37.0 35.0 37.0 29.0 37.0 7 33.82458309025281 37.0 35.0 37.0 29.0 37.0 8 33.85885867421175 37.0 35.0 37.0 30.0 37.0 9 35.47672526506025 39.0 37.0 39.0 30.0 39.0 10 35.390843972308005 39.0 37.0 39.0 30.0 39.0 11 35.39527795786779 39.0 37.0 39.0 30.0 39.0 12 35.307416768080024 39.0 37.0 39.0 30.0 39.0 13 35.301020593880565 39.0 37.0 39.0 30.0 39.0 14 36.50547386191583 40.0 37.0 41.0 29.0 41.0 15 36.450816898138356 40.0 37.0 41.0 29.0 41.0 16 36.39875421435226 40.0 37.0 41.0 29.0 41.0 17 36.37018614475433 40.0 37.0 41.0 29.0 41.0 18 36.319317301988164 40.0 37.0 41.0 29.0 41.0 19 36.35587874471085 40.0 37.0 41.0 29.0 41.0 20 36.25898971634101 40.0 37.0 41.0 28.0 41.0 21 36.20107391582806 40.0 37.0 41.0 28.0 41.0 22 36.104429853600756 40.0 37.0 41.0 27.0 41.0 23 36.009506457169785 40.0 37.0 41.0 27.0 41.0 24 35.92416674619048 40.0 37.0 41.0 27.0 41.0 25 35.83084497578377 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2681768412087102 1101 2 4.039767928269271 1101 3 2.23037734146693 1101 4 1.3314648027573242 1101 5 0.9579741181240138 1101 6 0.67653117302293 1101 7 0.42675567123765035 1101 8 0.24539983649849262 1101 9 0.31278685806915973 1101 10 0.6008595211872816 1101 11 0.593706784954648 1101 12 0.3200683166393148 1101 13 0.35717068874325264 1101 14 0.29735932715944813 1101 15 0.38590938624737703 1101 16 0.4619255526423416 1101 17 0.4988055379985141 1101 18 0.514945588938339 1101 19 0.6582913277380129 1101 20 0.5690953115398045 1101 21 0.7211210683000999 1101 22 0.7532173716310879 1101 23 0.6934891527168929 1101 24 0.48835890319606534 1101 25 0.5333570649772952 1102 1 1.8085931026744113 1102 2 4.4847927922848925 1102 3 2.799524371705978 1102 4 1.9811009833688544 1102 5 1.5151878846190812 1102 6 1.3600362078831765 1102 7 1.2139122567482374 1102 8 1.0667704785106409 1102 9 1.2437302452046382 1102 10 1.0076858652423155 1102 11 1.142256340842195 1102 12 1.1942256282334398 1102 13 1.1498480707074137 1102 14 1.2895700151944993 1102 15 1.1903242547061055 1102 16 1.1152040439499231 1102 17 1.1730922699425577 1102 18 1.1612303801870354 1102 19 1.2596699411166696 1102 20 1.3628494683756784 1102 21 1.3348376127279664 1102 22 1.2569202147009904 1102 23 1.411250936936824 1102 24 1.4144609202201508 1102 25 1.4174740104740664 1103 1 1.2733335248275637 1103 2 4.033329161611665 1103 3 2.412543852896899 1103 4 1.2644088157787081 1103 5 0.9733519521139584 1103 6 0.8947595527188668 1103 7 0.6200293908930021 1103 8 0.7188755498884731 1103 9 0.7229603182936941 1103 10 0.500823917717419 1103 11 0.6819132623986732 1103 12 0.6401157560992345 1103 13 0.6534154329041186 1103 14 0.7570577213026937 1103 15 0.7941674812617876 1103 16 0.6411542555449685 1103 17 0.8227544373200786 1103 18 0.84860769977346 1103 19 0.9087338344013531 1103 20 0.931420155450077 1103 21 0.9752486683904706 1103 22 0.7763709676263133 1103 23 1.0883250678737966 1103 24 1.1082902210288097 1103 25 1.1270722318465474 1104 1 1.49168674661912 1104 2 3.934683610686349 1104 3 2.3940317083409717 1104 4 1.7587434827134771 1104 5 1.267096968443063 1104 6 1.0254048817624835 1104 7 0.8632691409987387 1104 8 0.8968501860371632 1104 9 0.992704391625594 1104 10 0.9618621853760772 1104 11 0.8270813031139639 1104 12 0.917056839575288 1104 13 0.8470521432306342 1104 14 1.0426039316326694 1104 15 1.071517104409594 1104 16 1.221199325264351 1104 17 0.9435232573948227 1104 18 0.9618292139773601 1104 19 1.0398789519639848 1104 20 0.8559121503745359 1104 21 0.9142281512674444 1104 22 0.8727341879617683 1104 23 1.2019354297489784 1104 24 1.2123036448713833 1104 25 1.1873328041626507 1105 1 1.4128118486975687 1105 2 4.176093478708793 1105 3 2.42789382999214 1105 4 1.4652965239926843 1105 5 1.0344911361414901 1105 6 0.7875541928975949 1105 7 0.616503249689238 1105 8 0.6196938940532846 1105 9 0.5717615818998212 1105 10 0.5539461663038736 1105 11 0.5676247556046761 1105 12 0.5652215349205107 1105 13 0.6369962964405076 1105 14 0.5573187705462885 1105 15 0.4962164010561523 1105 16 0.5446989696362081 1105 17 0.7940303567863083 1105 18 0.7001074469006241 1105 19 0.654520821250884 1105 20 0.6681191522975567 1105 21 0.7285254563963903 1105 22 0.7981023289485734 1105 23 0.7147907286083282 1105 24 0.7285250299771562 1105 25 0.718497947335905 1106 1 0.3935436131568366 1106 2 2.2297853883413197 1106 3 1.158771507548856 1106 4 0.4525373484222399 1106 5 0.06378113533999397 1106 6 -0.11732331202954072 1106 7 -0.2963753280074286 1106 8 -0.30334876394902466 1106 9 -0.42176737773979056 1106 10 -0.4131309066382869 1106 11 -0.45064366695142866 1106 12 -0.2148617383007334 1106 13 -0.1836801032462958 1106 14 -0.21943162146671114 1106 15 -0.49621615523855667 1106 16 -0.5029473082439821 1106 17 -0.5273987284207777 1106 18 -0.5705130536840599 1106 19 -0.45804496808759865 1106 20 -0.4205656980182084 1106 21 -0.47110112938099036 1106 22 -0.4551832846365542 1106 23 -0.4785472081804727 1106 24 -0.4763893642471544 1106 25 -0.41589829098334263 1107 1 1.3398575588577266 1107 2 3.9709768090134467 1107 3 2.2194158157589783 1107 4 1.376590720221735 1107 5 0.8819770036620795 1107 6 0.7400427862309016 1107 7 0.4666061429798134 1107 8 0.40298269759340144 1107 9 0.40395686796281893 1107 10 0.2457274726311951 1107 11 0.37930670483088846 1107 12 0.30051270574302436 1107 13 0.3509394423721659 1107 14 0.4356745897880714 1107 15 0.4163729265535565 1107 16 0.4752076897811932 1107 17 0.5013925067895002 1107 18 0.4155910453663054 1107 19 0.31964122359065783 1107 20 0.5117166848231349 1107 21 0.5590707662795467 1107 22 0.5117392506956477 1107 23 0.46264421249165366 1107 24 0.5073157514089175 1107 25 0.46359958822603886 1108 1 0.8578886488061528 1108 2 3.1082053120333306 1108 3 1.658715481816568 1108 4 1.0039604961832822 1108 5 0.6347054751779595 1108 6 0.2826575733088603 1108 7 0.22616490694283442 1108 8 0.08402539001534137 1108 9 0.0650910570048282 1108 10 0.011700451199473605 1108 11 0.06364336558680606 1108 12 0.0059304938021043085 1108 13 0.05968720333780908 1108 14 0.0031948648630049092 1108 15 0.15069226010891867 1108 16 0.2306479806981372 1108 17 0.0825567146438928 1108 18 0.05352838840887131 1108 19 0.12783023859556408 1108 20 0.19751018059709935 1108 21 0.12691800787923313 1108 22 0.14395636726945327 1108 23 0.28569321471493936 1108 24 0.13043428746299668 1108 25 0.17628804966064138 1109 1 0.552689702865468 1109 2 2.703619783461189 1109 3 1.3862199929353771 1109 4 0.6670760032295888 1109 5 0.049654512892125524 1109 6 0.032983950815825835 1109 7 -0.25904792996923476 1109 8 -0.16633954958651032 1109 9 -0.1912984020573063 1109 10 -0.30879752116679526 1109 11 -0.257466860822241 1109 12 -0.14887455865095944 1109 13 -0.2783524290167776 1109 14 -0.24761300025324573 1109 15 -0.1262790169470236 1109 16 -0.11778230506637044 1109 17 -0.19778411395427042 1109 18 -0.2113206032892876 1109 19 -0.1595497880489063 1109 20 -0.16633158641718637 1109 21 -0.19350348658937122 1109 22 -0.3375891928762158 1109 23 -0.12412469464216258 1109 24 -0.2853107666790464 1109 25 -0.017283756837777275 1110 1 0.7701200593381472 1110 2 3.3740899186640867 1110 3 1.7848872020536106 1110 4 0.9527158595429981 1110 5 0.4409992566310663 1110 6 0.456903541832574 1110 7 0.1819910540063674 1110 8 0.08656777004046035 1110 9 -0.15719459827339222 1110 10 -0.09420872783768885 1110 11 -0.06920399888562656 1110 12 -0.07855643603798512 1110 13 -0.07174398127978066 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-0.5108637602003299 2315 14 -0.7740027738646873 2315 15 -0.9737023987496869 2315 16 -0.9120505152279605 2315 17 -0.929931576202641 2315 18 -0.7799019075160487 2315 19 -0.7961699911689522 2315 20 -0.7227821505701613 2315 21 -0.7494281765568758 2315 22 -0.8659551733900344 2315 23 -0.9568210277396503 2315 24 -0.9364036225046632 2315 25 -0.8588777048212393 2316 1 -0.7361771746336068 2316 2 -0.23657265196174393 2316 3 -0.21096701457000222 2316 4 -0.5031988714527742 2316 5 -0.9568091556899887 2316 6 -1.0837292518799657 2316 7 -1.0622021048507193 2316 8 -1.1548770277475455 2316 9 -1.2349675733232246 2316 10 -1.385693662709265 2316 11 -1.4538529366251183 2316 12 -1.357680178323605 2316 13 -1.5661472594846586 2316 14 -1.6278446213272275 2316 15 -1.484792304789373 2316 16 -1.6327067988686963 2316 17 -1.7870365005476856 2316 18 -1.810420427455341 2316 19 -1.6774896552230842 2316 20 -1.6263091986244262 2316 21 -1.7824911052702745 2316 22 -1.8676424550623736 2316 23 -1.751583441697278 2316 24 -1.9391530225156899 2316 25 -1.6930703630181299 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68610.0 3 1.0 4 650.0 5 2765.0 6 3343.0 7 3136.0 8 2841.0 9 2662.0 10 2665.0 11 2529.0 12 2362.0 13 2419.0 14 2559.0 15 2645.0 16 2769.0 17 3000.0 18 3495.0 19 4248.0 20 5545.0 21 7088.0 22 8612.0 23 10591.0 24 12144.0 25 13696.0 26 15553.0 27 17417.0 28 20226.0 29 24831.0 30 30969.0 31 39370.0 32 51342.0 33 68343.0 34 95089.0 35 139441.0 36 229089.0 37 319791.0 38 811098.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.76067693294519 19.651449579769928 13.755340802997049 24.832532684287834 2 16.48551130861983 22.127131514305226 39.523498894109736 21.86385828296521 3 17.931324873311187 25.54736159658897 30.577283866569203 25.944029663530642 4 11.942197828360388 17.910271558250294 39.71639020253485 30.431140410854457 5 12.83263499946383 38.133554753868054 36.34658085309213 12.687229393575986 6 29.970126875924464 38.27232699978504 19.197208365655303 12.560337758635193 7 26.735939287748643 32.33169399498459 23.30346189300784 17.628904824258928 8 24.140047901234325 37.429172530526095 21.55176781526986 16.879011752969724 9 25.832515959691754 16.19531180057985 21.148645258527825 36.82352698120057 10 14.528129814474857 29.53635438863671 35.03875191103293 20.896763885855503 11 32.73903628942209 23.37473818628642 25.687799013642355 18.198426510649142 12 22.7563708413554 25.161958037004645 32.443797978685 19.637873142954962 13 27.923238039208353 22.712542561637513 26.561314828715542 22.802904570438585 14 20.552118268473055 22.873984103763327 28.54337622372394 28.030521404039675 15 22.313808515180522 31.315773163319616 24.149136174612654 22.22128214688721 16 21.462083864995122 28.88623044329034 28.35930728690651 21.292378404808026 17 21.15617132676221 29.188994822261815 28.364078715787134 21.290755135188846 18 20.81674394444264 28.188089482843942 30.486549928526664 20.508616644186752 19 22.83632550042574 27.624957019930456 28.178151711898014 21.360565767745786 20 22.770414816498214 28.13835054254391 28.764394913137387 20.326839727820484 21 22.404875056508526 27.507539799466667 27.77361564559425 22.31396949843055 22 22.355158245080528 28.63321250376926 28.0849784863035 20.926650764846713 23 21.829940100909088 28.267186247951837 28.312539751952738 21.59033389918634 24 22.244915225890487 27.577607121143256 28.99855971572094 21.178917937245316 25 22.20169351958704 28.00446253331405 28.47511419500759 21.31872975209132 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 287.0 1 287.0 2 425.0 3 563.0 4 563.0 5 563.0 6 1280.0 7 1997.0 8 1997.0 9 1997.0 10 3061.0 11 4125.0 12 4125.0 13 4125.0 14 7712.0 15 11299.0 16 11299.0 17 11299.0 18 20190.5 19 29082.0 20 29082.0 21 29082.0 22 45246.0 23 61410.0 24 61410.0 25 61410.0 26 86468.5 27 111527.0 28 111527.0 29 111527.0 30 142200.5 31 172874.0 32 172874.0 33 172874.0 34 193572.5 35 214271.0 36 214271.0 37 214271.0 38 229017.5 39 243764.0 40 243764.0 41 243764.0 42 253984.5 43 264205.0 44 264205.0 45 264205.0 46 262254.5 47 260304.0 48 260304.0 49 260304.0 50 246519.5 51 232735.0 52 232735.0 53 232735.0 54 203888.0 55 175041.0 56 175041.0 57 175041.0 58 147148.0 59 119255.0 60 119255.0 61 119255.0 62 94760.5 63 70266.0 64 70266.0 65 70266.0 66 53023.5 67 35781.0 68 35781.0 69 35781.0 70 25710.5 71 15640.0 72 15640.0 73 15640.0 74 10665.0 75 5690.0 76 5690.0 77 5690.0 78 3813.0 79 1936.0 80 1936.0 81 1936.0 82 1272.0 83 608.0 84 608.0 85 608.0 86 399.0 87 190.0 88 190.0 89 190.0 90 118.5 91 47.0 92 47.0 93 47.0 94 39.0 95 31.0 96 31.0 97 31.0 98 18.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.47842084396247 3 0.0 4 0.0 5 0.0 6 1.4756996538008613E-4 7 0.0 8 4.9189988460028705E-5 9 0.0 10 9.837997692005741E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 3.9351990768022964E-4 19 7.378498269004307E-4 20 5.410898730603158E-4 21 0.002705449365301579 22 0.0021151695037812343 23 7.378498269004307E-4 24 9.837997692005741E-5 25 3.9351990768022964E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2032934.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.2624388375845 #Duplication Level Percentage of deduplicated Percentage of total 1 85.37095272090241 65.10597060384139 2 10.530044411141633 16.060937357234746 3 2.3539448694118317 5.385527298916968 4 0.7701062752654723 2.3492073086349237 5 0.3340461749454669 1.273758799285386 6 0.17737810480533495 0.8116372121306106 7 0.09977977605863401 0.5326614348229617 8 0.06882530987762618 0.41990287880162136 9 0.04718370265098802 0.3238509813796573 >10 0.21900188404103568 2.943037440066729 >50 0.015115327157485195 0.8175888048401971 >100 0.01108101516572805 1.76416246572179 >500 0.0016284798566867092 0.8645242746774542 >1k 9.119487197444915E-4 1.3472331396455381 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3722 0.18308513704822685 No Hit GTCCTACAGTGGACATTTCTAAATT 3019 0.14850457516082666 No Hit CTGTAGGACGTGGAATATGGCAAGA 2755 0.1355184182073791 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2619 0.12882857977681517 No Hit CTTTAGGACGTGAAATATGGCGAGG 2581 0.1269593602153341 No Hit TATCAACGCAGAGTACTTTTTTTTT 2204 0.10841473456590328 No Hit GGTATCAACGCAGAGTACTTTTTTT 2183 0.10738174480824268 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.9189988460028705E-5 0.0 5 0.0 0.0 0.0 4.9189988460028705E-5 0.0 6 0.0 0.0 0.0 4.9189988460028705E-5 0.0 7 0.0 0.0 0.0 4.9189988460028705E-5 0.0 8 0.0 0.0 0.0 4.9189988460028705E-5 0.0 9 0.0 0.0 0.0 1.9675995384011482E-4 0.0 10 0.0 0.0 0.0 2.4594994230014354E-4 0.0 11 0.0 0.0 0.0 3.44329919220201E-4 0.0 12 0.0 0.0 0.0 3.44329919220201E-4 0.0 13 0.0 0.0 0.0 3.44329919220201E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 115 5.6243152E-9 12.346166 18 TGGACCG 55 0.0031546094 12.046568 5 ACCGTTC 65 8.2764355E-4 11.649428 8 GGCCTAC 135 3.5695848E-8 10.895777 1 GGCGAGG 825 0.0 10.670079 19 TAGGACC 560 0.0 10.648306 4 GTCCTAT 195 7.2759576E-12 10.560523 1 TGGCGAG 1685 0.0 10.167573 18 GGCGTGC 85 6.8213075E-4 10.0219345 8 CCAACGA 125 2.693152E-6 9.84401 19 AGGACGT 1790 0.0 9.835307 5 ATTTAGA 610 0.0 9.8062 1 TCTAGAC 165 1.0855729E-8 9.751984 3 GGACGTG 1750 0.0 9.735594 6 ATTACCC 90 0.0011518787 9.46516 3 GTAGAAC 580 0.0 9.46516 3 CTAGGAC 290 0.0 9.46516 3 GTCCTAC 1665 0.0 9.364478 1 TTAGACA 285 0.0 9.299106 4 GTATCAA 2220 0.0 9.276135 1 >>END_MODULE