FastQCFastQC Report
Thu 26 May 2016
SRR1511985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032934
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44360.21820678880868732No Hit
GTCCTACAGTGGACATTTCTAAATT32850.16158911209119428No Hit
CTGTAGGACGTGGAATATGGCAAGA30240.1487505251031268No Hit
CTTTAGGACGTGAAATATGGCGAGG29580.1455039858647649No Hit
GTCCTAAAGTGTGTATTTCTCATTT29460.14491370600324457No Hit
TATCAACGCAGAGTACTTTTTTTTT27160.13360000865743799No Hit
GGTATCAACGCAGAGTACTTTTTTT26320.12946804962679556No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAAT456.793074E-414.76930417
CCAACGA903.6649E-813.71536519
TAGGACC6300.012.5228644
AGCACCG550.00305619512.0976625
CTCGGAT550.003082216712.08397612
GGCGAGG9350.011.27243919
TCGAACT1354.7675712E-911.253633519
AGGACGC600.005852335611.0895245
AGGACGT20100.010.9712995
GGAGCGC700.001491228210.8575936
TGGCGAG19400.010.62045718
GGACGTG20100.010.5875046
TGTAGGA20050.010.5245792
GACGTGG9300.010.5154717
CTCGAAC1451.4562829E-810.47700518
GTAGGAC18800.010.4659483
CGGTTTC1106.088545E-610.35769413
AGGACCT13800.010.3318555
GGACCTG12900.010.3105056
TCCAACG1303.8714643E-710.22515418
Produced by FastQC (version 0.11.5)