FastQCFastQC Report
Thu 26 May 2016
SRR1511984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704422
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41360.24266290859892678No Hit
GGTATCAACGCAGAGTACTTTTTTT25290.1483787465780188No Hit
TATCAACGCAGAGTACTTTTTTTTT24790.14544520077774167No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG551.4953285E-414.2659191
TTCAACG705.22333E-614.011172
GGTTTAC751.0650121E-513.0770921
CGATATT600.004633181711.4424551
ATAAGAC1604.9112714E-1111.2395163
TAGGACC4250.011.1351234
GTATTAG1155.84374E-711.08711
GTCTTAG1802.2009772E-1010.3526971
GTATAAG1656.0572347E-910.1050251
TAATACT2600.09.8288894
TCTATAC1651.0855729E-89.7516693
TTAGACT1856.9303496E-109.7206624
CTAGGAC2151.0913936E-119.6849683
TAGACAG2450.09.6580165
GGCGAGG5400.09.64069719
GGTATCA12250.09.6076591
TAGGACT2107.094059E-119.4648564
GACGAAA1002.8596475E-49.46485611
TAGGGCT1607.0223905E-89.4648554
TGGCGAG11550.09.42443618
Produced by FastQC (version 0.11.5)