FastQCFastQC Report
Thu 26 May 2016
SRR1511984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704422
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50130.2941173019357882No Hit
TATCAACGCAGAGTACTTTTTTTTT30780.18058907946506206No Hit
GGTATCAACGCAGAGTACTTTTTTT30640.17976768664098444No Hit
CTTTAGGACGTGAAATATGGCGAGG19740.11581638819494232No Hit
GTCCTACAGTGGACATTTCTAAATT19450.11411493163078158No Hit
GTACATAAGCAGTGGTATCAACGCA18280.10725043445813302No Hit
CTGTAGGACGTGGAATATGGCAAGA18280.10725043445813302No Hit
GTCCTAAAGTGTGTATTTCTCATTT17300.10150068468958978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAC250.006018167419.0077233
GGACCGT504.561747E-617.1004246
GAGTGCG752.0843284E-411.3939289
CCCGTTT600.005899403711.07742914
AGGACGT11800.010.3898145
GGACGTG11600.010.2373226
TATACTG2600.010.2349275
GGTATCA15050.010.2149121
TAGGACC4300.010.1669224
GACCGTG750.00265952510.1279357
GGCGAGG4550.010.01781519
TGTAGGA12050.010.0165182
TAGGACG12350.010.00406554
CTAGGAC2900.09.831583
GTAGGAC11400.09.75396253
GACGTGA6650.09.7091117
TAGGACA3650.09.6340514
GTACATA6250.09.6251131
ACAGTCG800.0045357179.494948
CTGTAGG11900.09.4684811
Produced by FastQC (version 0.11.5)