FastQCFastQC Report
Thu 26 May 2016
SRR1511983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588531
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34920.2198257383708596No Hit
GTCCTACAGTGGACATTTCTAAATT21250.13377139004526825No Hit
GGTATCAACGCAGAGTACTTTTTTT19890.12521002108237106No Hit
TATCAACGCAGAGTACTTTTTTTTT19660.12376214250776348No Hit
CTGTAGGACGTGGAATATGGCAAGA19570.12319558132639526No Hit
CTTTAGGACGTGAAATATGGCGAGG19100.1202368729348058No Hit
GTCCTAAAGTGTGTATTTCTCATTT18400.11583028596860873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG500.001179905113.7295021
CCGTAGG500.001179905113.7295021
CTAGTAC604.2212172E-412.6201043
CGGTAGG802.0697264E-512.2584841
TGCGTGG550.003154295712.04646210
TTAGGAC9550.011.1004063
GGCGAGG7800.010.92158619
AGGACGT14450.010.6768685
TAGAACG803.901277E-410.64821154
GGACGTG14300.010.5902966
TCCAACG1355.9981176E-810.51708318
CCCCTAC750.002033258810.4605731
TAGGACG15400.010.4484624
GACGTGA8550.010.406057
GAACAGT3400.010.3002316
TCTAGTA1157.0295337E-610.233172
CTCGAAC750.00272903310.096418
GCCGGTT750.002729745310.09608211
TAGGACC4250.010.0218474
GTAGGAC12950.010.01324753
Produced by FastQC (version 0.11.5)