##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511983_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1588531 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.615186609515334 31.0 30.0 34.0 25.0 34.0 2 26.64894232470125 31.0 25.0 34.0 10.0 34.0 3 28.62599344929372 31.0 28.0 34.0 16.0 34.0 4 33.15479206889888 35.0 33.0 37.0 28.0 37.0 5 33.62306243944877 35.0 35.0 37.0 28.0 37.0 6 33.911813493095195 37.0 35.0 37.0 30.0 37.0 7 33.99079652836488 37.0 35.0 37.0 30.0 37.0 8 34.02732524577739 37.0 35.0 37.0 30.0 37.0 9 35.65353461783245 39.0 37.0 39.0 30.0 39.0 10 35.58494798024086 39.0 37.0 39.0 30.0 39.0 11 35.58508962053621 39.0 37.0 39.0 30.0 39.0 12 35.5041387294299 39.0 37.0 39.0 30.0 39.0 13 35.492420985174356 39.0 37.0 39.0 30.0 39.0 14 36.722573245344286 40.0 37.0 41.0 30.0 41.0 15 36.663302132599235 40.0 37.0 41.0 30.0 41.0 16 36.60847789561551 40.0 37.0 41.0 30.0 41.0 17 36.57939505115103 40.0 37.0 41.0 30.0 41.0 18 36.54177790675788 40.0 37.0 41.0 30.0 41.0 19 36.57496013612577 40.0 37.0 41.0 30.0 41.0 20 36.4812225886684 40.0 37.0 41.0 29.0 41.0 21 36.43368621701433 40.0 37.0 41.0 29.0 41.0 22 36.336840766720954 40.0 37.0 41.0 29.0 41.0 23 36.24659071809112 40.0 37.0 41.0 29.0 41.0 24 36.15992133612753 40.0 37.0 41.0 29.0 41.0 25 36.06996401077473 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.271323551303631 1101 2 3.97811095607511 1101 3 2.2049892431994067 1101 4 1.3720005962259947 1101 5 0.8892745621479037 1101 6 0.6030321436728698 1101 7 0.1818705049525633 1101 8 0.15444610506764178 1101 9 0.21946437986863288 1101 10 0.40281406198427305 1101 11 0.3779989791386953 1101 12 0.370243504315809 1101 13 0.43739718209682366 1101 14 0.32602490465126266 1101 15 0.5869597519713494 1101 16 0.5922012194657995 1101 17 0.5800191733017925 1101 18 0.5821669092977828 1101 19 0.45429744515744375 1101 20 0.5562619718938677 1101 21 0.38191295205461984 1101 22 0.6722095757618476 1101 23 0.599030533250108 1101 24 0.39433963394976246 1101 25 0.47011728040622813 1102 1 1.8133403580263234 1102 2 4.472593597616573 1102 3 2.81697039929821 1102 4 1.9693395318002231 1102 5 1.4880777197745871 1102 6 1.3109728957312754 1102 7 1.0488597248829592 1102 8 1.0966623855192097 1102 9 1.1517281944853863 1102 10 1.033320810138079 1102 11 1.1977723430297047 1102 12 1.1142592924492476 1102 13 1.263706493700255 1102 14 1.3937823106439566 1102 15 1.3125136098781525 1102 16 1.3252671731199896 1102 17 1.3469107784286862 1102 18 1.4176647447956228 1102 19 1.3382207114336566 1102 20 1.417988480679199 1102 21 1.5256189192900038 1102 22 1.442284272307134 1102 23 1.3801611673219227 1102 24 1.4116980924846914 1102 25 1.528091985439758 1103 1 1.0785118217071634 1103 2 3.796294740198949 1103 3 2.22394190724744 1103 4 1.322435266006714 1103 5 0.8179668961535853 1103 6 0.761768200229092 1103 7 0.4990005441534464 1103 8 0.5825270311049664 1103 9 0.5903019891258552 1103 10 0.45581487588426484 1103 11 0.42928647456788127 1103 12 0.25494690264318365 1103 13 0.44073043679247803 1103 14 0.49885676549401836 1103 15 0.707393360143378 1103 16 0.5017167720331202 1103 17 0.4619437125832775 1103 18 0.4708306978253347 1103 19 0.6032509742199679 1103 20 0.6245882868141877 1103 21 0.7219511306486552 1103 22 0.5669264997703252 1103 23 0.6921483600191607 1103 24 0.9105360472851416 1103 25 1.0408096065481445 1104 1 1.3907164633794729 1104 2 3.935088681345519 1104 3 2.1652505815095253 1104 4 1.534759173618717 1104 5 1.1763058125937818 1104 6 0.837675402706374 1104 7 0.6348155310683197 1104 8 0.7505712133878717 1104 9 0.746298784751815 1104 10 0.7402538140581711 1104 11 0.7042124273033608 1104 12 0.7768189108912154 1104 13 0.7564641109198504 1104 14 0.7108420943464964 1104 15 0.8695806687386067 1104 16 0.9034229575168027 1104 17 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1107 16 0.4944997915404912 1107 17 0.5122761783199863 1107 18 0.6401238777025142 1107 19 0.631000509920213 1107 20 0.8009476344899724 1107 21 0.7267822204313532 1107 22 0.7665296420968346 1107 23 0.7610612885644485 1107 24 0.6814888446064344 1107 25 0.7205749487743418 1108 1 1.0448322327545512 1108 2 3.373123484661644 1108 3 2.0054721537906204 1108 4 1.1693188014864404 1108 5 0.6593606733892443 1108 6 0.543999319297086 1108 7 0.39321551774161634 1108 8 0.3135747822468602 1108 9 0.40508802217097184 1108 10 0.4294366571989272 1108 11 0.3879931967141417 1108 12 0.18414320269204154 1108 13 0.2643359873749773 1108 14 0.2575057783704011 1108 15 0.4125727671150514 1108 16 0.41020331364117624 1108 17 0.38787337285273793 1108 18 0.2554677360282156 1108 19 0.4286099357154285 1108 20 0.4857007641485396 1108 21 0.4909689582225383 1108 22 0.31220155329894794 1108 23 0.4222993222228695 1108 24 0.38490542595959454 1108 25 0.6231379460622719 1109 1 0.9289936492197768 1109 2 3.152015454232682 1109 3 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-1.1859541127690179 2315 14 -1.148606058946605 2315 15 -1.5097676597008984 2315 16 -1.456379587891341 2315 17 -1.4082110295723425 2315 18 -1.4501768753027662 2315 19 -1.3267433684811607 2315 20 -1.1355559059821871 2315 21 -1.217158432372834 2315 22 -1.4269768724717409 2315 23 -1.2557915086331164 2315 24 -1.2064504708523813 2315 25 -1.1833307983176766 2316 1 -0.8019683741622075 2316 2 -0.20602857589051027 2316 3 -0.34645062128393533 2316 4 -0.6852112740293563 2316 5 -0.9857866085654408 2316 6 -1.149066482338796 2316 7 -1.2068176185181727 2316 8 -1.3320085031260973 2316 9 -1.405029001146488 2316 10 -1.399432001131359 2316 11 -1.5446686913550636 2316 12 -1.3127228047374544 2316 13 -1.565118659549455 2316 14 -1.6286083847646395 2316 15 -1.4585638065956843 2316 16 -1.5624383438694167 2316 17 -1.780655900416761 2316 18 -1.7298162765984415 2316 19 -1.602318402762748 2316 20 -1.5502817259541786 2316 21 -1.5529261219148864 2316 22 -1.7360029846246263 2316 23 -1.7649571698680262 2316 24 -1.9310697392856966 2316 25 -1.7895916097374425 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48710.0 3 2.0 4 409.0 5 2034.0 6 2388.0 7 2188.0 8 2050.0 9 1889.0 10 1936.0 11 1885.0 12 1745.0 13 1779.0 14 1917.0 15 1977.0 16 2034.0 17 2198.0 18 2645.0 19 3221.0 20 4091.0 21 5356.0 22 6541.0 23 7841.0 24 9143.0 25 10362.0 26 11832.0 27 13447.0 28 15735.0 29 18830.0 30 23593.0 31 30747.0 32 39273.0 33 52789.0 34 72640.0 35 108323.0 36 179262.0 37 248752.0 38 648967.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.65043049207098 19.81654748947298 14.017541993200005 24.51548002525604 2 16.48693388085849 22.25636282759871 39.348305050476064 21.908398241066735 3 17.74079322342466 25.905821164333588 30.75325568087749 25.60012993136426 4 11.969549225038731 18.244403162418614 39.86765130803239 29.91839630451027 5 12.819453948333399 38.13894724119328 36.22201895965518 12.819579850818148 6 29.499096649103258 38.448250898629546 19.383329241500004 12.669323210767185 7 26.454693046594617 32.60622550016336 23.383679638609507 17.555401814632514 8 24.150126217320416 37.03071393048919 21.722347075598194 17.096812776592195 9 25.73981873819271 16.420327963382523 21.3071699576527 36.532683340772074 10 14.63070551434692 29.538900454445592 34.84972575256731 20.980668278640177 11 32.398108692874104 23.6320222897759 25.83814857878128 18.131720438568717 12 22.737799891849768 25.29173179497284 32.30015655973978 19.670311753437613 13 27.695147277579096 22.81705550599894 26.871430271112118 22.61636694530985 14 20.714119343040423 22.98880096693169 28.53795647548362 27.759123214544264 15 22.306583881586196 31.089541217640697 24.41167342658091 22.192201474192196 16 21.4154460945364 29.027069663733347 28.30262676649055 21.254857475239703 17 20.951558389480596 29.227380516968193 28.53435028967014 21.28671080388107 18 20.757773101176376 28.44132568429919 30.40812125477566 20.392779959748772 19 22.584229345554352 27.87336640394833 28.375217183290108 21.16718706720721 20 22.497810854009302 28.2846475238886 28.92625143293848 20.291290189163615 21 22.276390008058016 27.61312701450443 28.067523670427075 22.042959307010477 22 22.23568005308149 28.523998411080637 28.356167229356483 20.884154306481392 23 21.84385465716516 28.273298416135773 28.301941429758514 21.58090549694055 24 22.01788948405791 27.80392702440179 28.953164905185986 21.22501858635431 25 22.08038536426605 28.08212408633681 28.529457867465563 21.30803268193158 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 316.0 1 316.0 2 412.0 3 508.0 4 508.0 5 508.0 6 1122.5 7 1737.0 8 1737.0 9 1737.0 10 2680.0 11 3623.0 12 3623.0 13 3623.0 14 6288.0 15 8953.0 16 8953.0 17 8953.0 18 15898.0 19 22843.0 20 22843.0 21 22843.0 22 35896.0 23 48949.0 24 48949.0 25 48949.0 26 68879.5 27 88810.0 28 88810.0 29 88810.0 30 112962.5 31 137115.0 32 137115.0 33 137115.0 34 152952.0 35 168789.0 36 168789.0 37 168789.0 38 180720.0 39 192651.0 40 192651.0 41 192651.0 42 201128.0 43 209605.0 44 209605.0 45 209605.0 46 206507.5 47 203410.0 48 203410.0 49 203410.0 50 191712.5 51 180015.0 52 180015.0 53 180015.0 54 157056.5 55 134098.0 56 134098.0 57 134098.0 58 111431.0 59 88764.0 60 88764.0 61 88764.0 62 70946.0 63 53128.0 64 53128.0 65 53128.0 66 40104.5 67 27081.0 68 27081.0 69 27081.0 70 19446.0 71 11811.0 72 11811.0 73 11811.0 74 8012.5 75 4214.0 76 4214.0 77 4214.0 78 2845.0 79 1476.0 80 1476.0 81 1476.0 82 953.5 83 431.0 84 431.0 85 431.0 86 289.5 87 148.0 88 148.0 89 148.0 90 91.0 91 34.0 92 34.0 93 34.0 94 25.0 95 16.0 96 16.0 97 16.0 98 11.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.483029289324539 3 0.0 4 0.0 5 0.0 6 6.295124237424389E-5 7 0.0 8 6.295124237424389E-5 9 0.0 10 1.2590248474848777E-4 11 0.0 12 0.0 13 0.0 14 6.295124237424389E-5 15 0.0 16 0.0 17 0.0 18 5.036099389939511E-4 19 6.924636661166826E-4 20 7.554149084909265E-4 21 0.0032105133610864376 22 0.0018885372712273165 23 6.924636661166826E-4 24 0.0 25 5.036099389939511E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1588531.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.46622258912956 #Duplication Level Percentage of deduplicated Percentage of total 1 85.9011553063436 66.54438017624601 2 10.2933315798556 15.947710306976207 3 2.224568866377975 5.169868409030514 4 0.7122581649037555 2.2070379817343704 5 0.30756362267698445 1.1912896027307167 6 0.1539334076243884 0.7154783771360449 7 0.09452823254291273 0.5125921572188394 8 0.061887490811236054 0.3835352110932737 9 0.03571379903417506 0.24899517949363603 >10 0.18740343036300905 2.4884296855107393 >50 0.013609894740427064 0.7446940395098702 >100 0.011582186820924406 1.832542420866576 >500 0.001806946463733969 0.9444651332032301 >1k 6.570714413578069E-4 1.0689813192500315 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3492 0.2198257383708596 No Hit GTCCTACAGTGGACATTTCTAAATT 2125 0.13377139004526825 No Hit GGTATCAACGCAGAGTACTTTTTTT 1989 0.12521002108237106 No Hit TATCAACGCAGAGTACTTTTTTTTT 1966 0.12376214250776348 No Hit CTGTAGGACGTGGAATATGGCAAGA 1957 0.12319558132639526 No Hit CTTTAGGACGTGAAATATGGCGAGG 1910 0.1202368729348058 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1840 0.11583028596860873 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.2590248474848774E-4 0.0 0.0 0.0 0.0 7 1.2590248474848774E-4 0.0 0.0 6.295124237424387E-5 0.0 8 1.2590248474848774E-4 0.0 0.0 6.295124237424387E-5 0.0 9 1.2590248474848774E-4 0.0 0.0 6.295124237424387E-5 0.0 10 1.2590248474848774E-4 0.0 0.0 6.295124237424387E-5 0.0 11 1.2590248474848774E-4 0.0 0.0 1.8885372712273163E-4 0.0 12 1.2590248474848774E-4 0.0 0.0 1.8885372712273163E-4 0.0 13 1.2590248474848774E-4 0.0 0.0 1.8885372712273163E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAGG 50 0.0011799051 13.729502 1 CCGTAGG 50 0.0011799051 13.729502 1 CTAGTAC 60 4.2212172E-4 12.620104 3 CGGTAGG 80 2.0697264E-5 12.258484 1 TGCGTGG 55 0.0031542957 12.046462 10 TTAGGAC 955 0.0 11.100406 3 GGCGAGG 780 0.0 10.921586 19 AGGACGT 1445 0.0 10.676868 5 TAGAACG 80 3.901277E-4 10.6482115 4 GGACGTG 1430 0.0 10.590296 6 TCCAACG 135 5.9981176E-8 10.517083 18 CCCCTAC 75 0.0020332588 10.460573 1 TAGGACG 1540 0.0 10.448462 4 GACGTGA 855 0.0 10.40605 7 GAACAGT 340 0.0 10.300231 6 TCTAGTA 115 7.0295337E-6 10.23317 2 CTCGAAC 75 0.002729033 10.0964 18 GCCGGTT 75 0.0027297453 10.096082 11 TAGGACC 425 0.0 10.021847 4 GTAGGAC 1295 0.0 10.0132475 3 >>END_MODULE