FastQCFastQC Report
Thu 26 May 2016
SRR1511983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588531
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42190.2655912915769349No Hit
GGTATCAACGCAGAGTACTTTTTTT25180.15851122829834607No Hit
TATCAACGCAGAGTACTTTTTTTTT24030.15127183542530803No Hit
GTCCTACAGTGGACATTTCTAAATT23560.14831312703371857No Hit
CTTTAGGACGTGAAATATGGCGAGG22870.14396949130989573No Hit
CTGTAGGACGTGGAATATGGCAAGA21980.13836683073858805No Hit
GTCCTAAAGTGTGTATTTCTCATTT21190.13339368259102277No Hit
GTACATAAGCAGTGGTATCAACGCA17010.10708006327858884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC658.0658623E-411.68498613
GTGCGCC752.0859698E-411.39286111
CTTACAC1850.011.3051683
TAGGACC4100.011.12974
GCTGCGA600.005903213311.0763938
TGCGCCA803.7926604E-410.68080812
GCCGGTT856.63119E-410.05252511
CCGGTTT856.63119E-410.05252512
GATATAC4850.010.0403211
GGTCTAT1053.8825274E-510.0028221
GGCGAGG5450.09.93018219
ATTACTC1151.017724E-59.919953
CCAACGA1252.5876998E-69.87443419
CCTATAC1356.379105E-79.8587163
GTTCTAA1553.7553036E-89.8561531
TGGCGAG11600.09.8220518
TAGGACA4550.09.8200244
GGTATCA13150.09.6570631
TGTAGGA13450.09.612642
AATGCCG1701.6787453E-89.49464918
Produced by FastQC (version 0.11.5)