FastQCFastQC Report
Thu 26 May 2016
SRR1511982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583947
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37460.23649781211113755No Hit
GGTATCAACGCAGAGTACTTTTTTT22240.14040873842369725No Hit
TATCAACGCAGAGTACTTTTTTTTT21970.13870413593384123No Hit
CTGTAGGACGTGGAATATGGCAAGA19770.12481478231279203No Hit
GTCCTAAAGTGTGTATTTCTCATTT19430.12266824584408444No Hit
GTCCTACAGTGGACATTTCTAAATT19350.12216317843968265No Hit
CTTTAGGACGTGAAATATGGCGAGG18780.1185645731833199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCG751.5354746E-512.6202625
GGTTATA1103.2969547E-711.5885481
CGGTAGG700.001144746811.2065081
TAGGACC4250.011.1355264
CCGGTTT951.4165849E-510.959702512
TCCAACG1053.6201363E-610.81770918
GCCGGTT909.8750344E-510.51688511
CCTTATA2250.010.4594072
AGGACCT8450.010.3053035
AGGACGT12600.010.2915245
GGACCTG8350.010.2020086
GACGTGG6300.010.0661627
GGACGTG12550.010.0308466
GGTATCA10350.09.9478041
CGGTTTC1054.2732012E-59.9159213
GTAGGAC12700.09.8378433
GTCCATA908.331237E-49.8056941
GACAGTC1554.29809E-89.7705267
TTAGGAC8000.09.7018263
TCCTATA2450.09.60557652
Produced by FastQC (version 0.11.5)