FastQCFastQC Report
Thu 26 May 2016
SRR1511982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583947
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44660.2819538785072985No Hit
TATCAACGCAGAGTACTTTTTTTTT28010.17683672496617628No Hit
GGTATCAACGCAGAGTACTTTTTTT26980.17033398213450324No Hit
GTCCTAAAGTGTGTATTTCTCATTT22260.1405350052747977No Hit
CTGTAGGACGTGGAATATGGCAAGA21990.13883040278494166No Hit
CTTTAGGACGTGAAATATGGCGAGG21890.13819906852943944No Hit
GTCCTACAGTGGACATTTCTAAATT21830.13782026797613808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4050.012.4371644
CGAGTAC550.003078015512.08590619
TGGACTG1900.011.0044825
CTGGACT2350.010.9193414
CGTGCGC700.001498164310.8512810
GCCGGTT700.001498164310.8512811
TGCGCCA1158.876559E-710.73333212
GTATTAG2100.010.4582391
GGCGAGG6000.010.44567619
CGAAATC750.002659507610.12786213
AAGGCGT951.6418264E-410.0012316
GTGCGCC1151.0327865E-59.90769111
GGTATCA11350.09.8432851
GACGTGA7650.09.80516057
AGGACCT8750.09.6667945
TTAGGAC9000.09.6094693
AGGACGT14200.09.5707995
GTATTAC1002.6166197E-49.5488271
TGGCGAG12750.09.53240618
TGTAGGA13100.09.5038712
Produced by FastQC (version 0.11.5)