FastQCFastQC Report
Thu 26 May 2016
SRR1511981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816100
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41970.23109960905236493No Hit
GGTATCAACGCAGAGTACTTTTTTT25380.1397500137657618No Hit
TATCAACGCAGAGTACTTTTTTTTT25120.138318374538847No Hit
GTCCTACAGTGGACATTTCTAAATT22090.12163427124057045No Hit
CTGTAGGACGTGGAATATGGCAAGA19910.1096305269533616No Hit
GTCCTAAAGTGTGTATTTCTCATTT18500.10186663729970817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT400.00427649414.7094381
GTCAACG551.4973962E-414.2636992
TGGACCG552.0227137E-413.7673245
TAGGACC3350.012.9967644
TCCAACG907.7702225E-611.56901418
CCAACGA600.0060373111.04314919
AATCCCG1304.037738E-710.19367719
TTAGGAC8550.010.1845983
AGGACGT13400.010.1007465
GGCGAGG6300.010.06654419
CTAGTAC1054.2749383E-59.91575053
CCTAGAC1252.693865E-69.84363653
TCTATAC2800.09.8030723
ATTACAC2054.3655746E-119.695893
GGACGTG13400.09.6063046
GACGTGG6950.09.5331297
GTCTAAG1552.6884663E-79.4899611
TAATACT1901.1241355E-99.46503454
CTAGTAG900.00115189459.46503453
GTAGGAC13850.09.3625263
Produced by FastQC (version 0.11.5)