##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511981_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1816100 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.391452563184846 31.0 30.0 34.0 25.0 34.0 2 26.449340344694676 31.0 25.0 34.0 10.0 34.0 3 28.4155376906558 31.0 28.0 34.0 16.0 34.0 4 32.917979736798635 35.0 33.0 37.0 27.0 37.0 5 33.38069269313364 35.0 35.0 37.0 28.0 37.0 6 33.667906502945876 37.0 35.0 37.0 28.0 37.0 7 33.742139199383296 37.0 35.0 37.0 29.0 37.0 8 33.78357414239304 37.0 35.0 37.0 29.0 37.0 9 35.39162546115302 39.0 37.0 39.0 30.0 39.0 10 35.313951324266284 39.0 35.0 39.0 30.0 39.0 11 35.31086283794945 39.0 35.0 39.0 29.0 39.0 12 35.22159737899895 39.0 35.0 39.0 29.0 39.0 13 35.20874180937173 39.0 35.0 39.0 29.0 39.0 14 36.409277572820876 40.0 37.0 41.0 29.0 41.0 15 36.34402015307527 40.0 37.0 41.0 29.0 41.0 16 36.29183029568856 40.0 37.0 41.0 28.0 41.0 17 36.266879577115795 40.0 37.0 41.0 28.0 41.0 18 36.22120533010297 40.0 37.0 41.0 28.0 41.0 19 36.250561643081326 40.0 37.0 41.0 28.0 41.0 20 36.15430427839877 40.0 37.0 41.0 27.0 41.0 21 36.099051263696936 40.0 37.0 41.0 27.0 41.0 22 35.99819228016079 40.0 37.0 41.0 27.0 41.0 23 35.89961345740873 40.0 36.0 41.0 27.0 41.0 24 35.809683387478664 40.0 36.0 41.0 27.0 41.0 25 35.71512692032377 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1336082054651335 1101 2 3.9526450488975016 1101 3 2.179746370001439 1101 4 1.2591772483566217 1101 5 0.8500883260933776 1101 6 0.42422812701324375 1101 7 0.2148274185940977 1101 8 0.2340894567763101 1101 9 0.05587553861772676 1101 10 0.31455845363790047 1101 11 0.39678067433926856 1101 12 0.19637321816549047 1101 13 0.31154600953833267 1101 14 0.22454237741702343 1101 15 0.34093388673836955 1101 16 0.578713925031451 1101 17 0.5188750156122737 1101 18 0.6565986506826675 1101 19 0.5530712611858775 1101 20 0.6900993285018586 1101 21 0.49984994036718433 1101 22 0.48922646956693683 1101 23 0.390366286985838 1101 24 0.4406471908295089 1101 25 0.20720662350755248 1102 1 1.745135122218528 1102 2 4.483672093861827 1102 3 2.6968670541952946 1102 4 1.813322609383512 1102 5 1.386718188681428 1102 6 1.2117807312034827 1102 7 1.0390528737428966 1102 8 0.999425369411874 1102 9 1.0762199735091968 1102 10 0.895724268078979 1102 11 0.915233620928106 1102 12 1.082360517741641 1102 13 1.1362507473092762 1102 14 1.1852653362236012 1102 15 1.1509543250689092 1102 16 1.1916549329201942 1102 17 1.2080476026071594 1102 18 1.2248886939727086 1102 19 1.2237603572234121 1102 20 1.2161921381312482 1102 21 1.3664943586666496 1102 22 1.2342006011264317 1102 23 1.2858589186282927 1102 24 1.2158591159543306 1102 25 1.1975719492387356 1103 1 1.4314163411458267 1103 2 4.068511539378587 1103 3 2.515033984066033 1103 4 1.641220778652979 1103 5 1.351071584013944 1103 6 1.1049418120948644 1103 7 0.9458830193824852 1103 8 0.8976984582578709 1103 9 0.9828541783404532 1103 10 0.9595044634348895 1103 11 0.9355515129559464 1103 12 0.9323751401900182 1103 13 0.9500952814380668 1103 14 1.1103954760142116 1103 15 1.2732170027316627 1103 16 0.9388532718100535 1103 17 1.2174308277343755 1103 18 1.0332220273060457 1103 19 1.126510839764208 1103 20 1.2392444280066641 1103 21 1.1276556735172107 1103 22 1.324739877022786 1103 23 1.346236470245536 1103 24 1.3699226943599 1103 25 1.4982387328849853 1104 1 1.404627027362885 1104 2 3.9093882314391486 1104 3 2.187354013657611 1104 4 1.4984964832193057 1104 5 1.006452305433342 1104 6 0.7373880215051543 1104 7 0.6513730467713188 1104 8 0.7013539740532053 1104 9 0.7059796640870388 1104 10 0.6376378665910494 1104 11 0.5447213694451065 1104 12 0.5958956236299571 1104 13 0.62764371063232 1104 14 0.5963748034707876 1104 15 0.575440735011 1104 16 0.6954459179704244 1104 17 0.8071905624076408 1104 18 0.6594496414019417 1104 19 0.6666028562723127 1104 20 0.6305286276833186 1104 21 0.5866015151108357 1104 22 0.8193036713945716 1104 23 0.846405832156357 1104 24 0.7000755564722851 1104 25 0.7144203802880327 1105 1 1.5536928551947042 1105 2 4.260635297019871 1105 3 2.5305833942488682 1105 4 1.5473455897867296 1105 5 1.199202327973687 1105 6 1.032452520291315 1105 7 0.9048113680330445 1105 8 0.6560058008231238 1105 9 0.7199426578458699 1105 10 0.46539154567661 1105 11 0.5805127693843275 1105 12 0.6322406812831574 1105 13 0.7061999930445069 1105 14 0.6889097744020916 1105 15 0.7685033170328239 1105 16 0.6457716677291572 1105 17 0.8546745587611184 1105 18 0.6241661506201055 1105 19 0.6070572696723033 1105 20 0.6148422156724891 1105 21 0.7090116292150768 1105 22 0.7192492406301696 1105 23 0.5867647412703363 1105 24 0.8680478936224532 1105 25 0.8713559616453281 1106 1 0.5190187563368056 1106 2 2.3221604039463095 1106 3 1.2589835884791647 1106 4 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0.45346249705784913 1107 16 0.6297284609696305 1107 17 0.6246082870691296 1107 18 0.7172516890893661 1107 19 0.5220303438570753 1107 20 0.6974842387625131 1107 21 0.7709836589855925 1107 22 0.7436370978245535 1107 23 0.7927204116870641 1107 24 0.804079872144527 1107 25 0.7340415176783139 1108 1 1.2151600521833181 1108 2 3.6234084016569383 1108 3 2.128133599210585 1108 4 1.532745493549129 1108 5 0.9830545118971372 1108 6 0.7304942679173507 1108 7 0.5487042397507622 1108 8 0.6336725872792073 1108 9 0.611644296930514 1108 10 0.6617077166384391 1108 11 0.6366637527742114 1108 12 0.47711237463850154 1108 13 0.704727192347697 1108 14 0.6373239858933459 1108 15 0.654623784576998 1108 16 0.7067144307227551 1108 17 0.5834806643666539 1108 18 0.5924071344363568 1108 19 0.6941663044335655 1108 20 0.8054292041057067 1108 21 0.7986636511182681 1108 22 0.8091485566123069 1108 23 0.9201189869929394 1108 24 0.7396498841570605 1108 25 0.753799300237219 1109 1 0.923544256232109 1109 2 3.1959772184682826 1109 3 1.8406500075769259 1109 4 1.0076862377009803 1109 5 0.6743669135357067 1109 6 0.5510772831892154 1109 7 0.31142355740307437 1109 8 0.30610423752248295 1109 9 0.22444317774746025 1109 10 0.16757812891929547 1109 11 0.3721002521916219 1109 12 0.2471988644143721 1109 13 0.2986292070192178 1109 14 0.4066760254471333 1109 15 0.42839208506082116 1109 16 0.4468288509113165 1109 17 0.3825310958868826 1109 18 0.4780120739623399 1109 19 0.5130234026532392 1109 20 0.4948228579532241 1109 21 0.5952274947538427 1109 22 0.6226446325340902 1109 23 0.6403193998582992 1109 24 0.657958206851923 1109 25 0.6171088001282357 1110 1 1.2079938743000262 1110 2 3.904073210523599 1110 3 2.1274332213027485 1110 4 1.3143413183657842 1110 5 0.9776281700704033 1110 6 0.7923713865076252 1110 7 0.4741616013361991 1110 8 0.3745771962313498 1110 9 0.3416671279807417 1110 10 0.12578108069529748 1110 11 0.23654324968704543 1110 12 0.2555924699406802 1110 13 0.3281867841395183 1110 14 0.3587666585862266 1110 15 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2315 19 -1.2532834447723573 2315 20 -1.308522908705875 2315 21 -1.200353360616269 2315 22 -1.5569876863159777 2315 23 -1.4771927004733953 2315 24 -1.17093969182784 2315 25 -1.093893648944551 2316 1 -0.5320435422884202 2316 2 0.09572808032207547 2316 3 -0.09287585664377573 2316 4 -0.3531568992813021 2316 5 -0.6958717042673115 2316 6 -0.8357317027331206 2316 7 -1.0157392536802945 2316 8 -0.9974344775390875 2316 9 -0.9600037958166183 2316 10 -1.1637961298069968 2316 11 -1.1662268344810442 2316 12 -1.1805858320283704 2316 13 -1.1980965099564003 2316 14 -1.4818877854991825 2316 15 -1.203583931999482 2316 16 -1.4136524630656453 2316 17 -1.5789481610286344 2316 18 -1.6342960654994059 2316 19 -1.527706016635733 2316 20 -1.4163123526224695 2316 21 -1.5439005680795503 2316 22 -1.5922782326274216 2316 23 -1.6803392040809015 2316 24 -1.7439195651658181 2316 25 -1.5823473729429978 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63057.0 3 3.0 4 531.0 5 2551.0 6 3008.0 7 2738.0 8 2494.0 9 2559.0 10 2351.0 11 2343.0 12 2208.0 13 2376.0 14 2315.0 15 2447.0 16 2560.0 17 2745.0 18 3137.0 19 3931.0 20 5137.0 21 6682.0 22 8156.0 23 9670.0 24 11202.0 25 12537.0 26 14289.0 27 16409.0 28 19030.0 29 22836.0 30 28680.0 31 36257.0 32 46506.0 33 62247.0 34 85476.0 35 125619.0 36 204754.0 37 284230.0 38 713029.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.79087054677606 19.745498595892297 13.962061560486758 24.501569296844888 2 16.516932647074594 22.19950413204281 39.159762814010726 22.12380040687187 3 17.70254941908485 25.79863443642971 30.64781675017895 25.850999394306477 4 12.024227740763175 18.15803094543252 39.72644678156489 30.091294532239417 5 12.954242607785915 38.17273277903199 36.04955674247013 12.823467870711966 6 29.663234403391886 38.3870381586917 19.286217719288583 12.66350971862783 7 26.591872694234898 32.49039149826552 23.43075821815979 17.486977589339794 8 24.302296129067784 36.99262155167667 21.636363636363637 17.068718682891912 9 25.851274709542427 16.2665602114421 21.29800121138704 36.58416386762843 10 14.64452103106714 29.455167366977243 34.753832252536895 21.146479349418726 11 32.397610263752 23.625681405208965 25.662023016353725 18.314685314685313 12 22.749022630912393 25.461153020208137 32.1360607896041 19.65376355927537 13 27.73685369748362 22.78106932437641 26.738615714993667 22.7434612631463 14 20.773966191289027 22.86762843455757 28.53328561202577 27.82511976212764 15 22.3438687296955 31.007158196134576 24.352293375915423 22.2966796982545 16 21.51781289576565 28.99069434502505 28.157700567149384 21.33379219205991 17 21.14454049887121 29.199878861296185 28.377787566763946 21.27779307306866 18 20.920007444548574 28.22607199847585 30.345345560306903 20.508574996668674 19 22.722271962891625 27.811403204473795 28.267273686378285 21.19905114625629 20 22.726889786910455 28.096609696320286 28.841010720277783 20.335489796491476 21 22.446262315230246 27.521029226164124 28.020411399723795 22.01229705888183 22 22.327247658127337 28.418098881759935 28.24448203481367 21.010171425299053 23 21.814586668120345 28.135498212361437 28.383724752668243 21.666190366849975 24 22.076923076923077 27.66108694455151 28.942844557017782 21.319145421507628 25 22.24187981666127 27.953484735354817 28.507972063089316 21.2966633848946 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 370.0 1 370.0 2 489.0 3 608.0 4 608.0 5 608.0 6 1414.0 7 2220.0 8 2220.0 9 2220.0 10 3449.5 11 4679.0 12 4679.0 13 4679.0 14 7870.0 15 11061.0 16 11061.0 17 11061.0 18 19367.0 19 27673.0 20 27673.0 21 27673.0 22 42384.5 23 57096.0 24 57096.0 25 57096.0 26 78586.0 27 100076.0 28 100076.0 29 100076.0 30 126032.0 31 151988.0 32 151988.0 33 151988.0 34 170837.0 35 189686.0 36 189686.0 37 189686.0 38 202531.0 39 215376.0 40 215376.0 41 215376.0 42 225280.0 43 235184.0 44 235184.0 45 235184.0 46 233067.5 47 230951.0 48 230951.0 49 230951.0 50 219084.5 51 207218.0 52 207218.0 53 207218.0 54 181928.5 55 156639.0 56 156639.0 57 156639.0 58 131367.0 59 106095.0 60 106095.0 61 106095.0 62 85108.0 63 64121.0 64 64121.0 65 64121.0 66 48431.5 67 32742.0 68 32742.0 69 32742.0 70 23508.0 71 14274.0 72 14274.0 73 14274.0 74 9825.0 75 5376.0 76 5376.0 77 5376.0 78 3599.5 79 1823.0 80 1823.0 81 1823.0 82 1197.0 83 571.0 84 571.0 85 571.0 86 380.0 87 189.0 88 189.0 89 189.0 90 123.0 91 57.0 92 57.0 93 57.0 94 38.5 95 20.0 96 20.0 97 20.0 98 13.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.4807554650074333 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 5.506304718903144E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 3.303782831341886E-4 19 7.708826606464402E-4 20 4.95567424701283E-4 21 0.0030835306425857608 22 0.0017620175100490061 23 9.360718022135345E-4 24 0.0 25 4.4050437751225153E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1816100.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.86220167848482 #Duplication Level Percentage of deduplicated Percentage of total 1 86.3099820697891 67.20285230784327 2 9.96823345439848 15.522972072091878 3 2.1691434627988424 5.066828573100312 4 0.6737668520361584 2.0984388207006885 5 0.3057921161937429 1.1904823711383938 6 0.15181775166049666 0.7092518638898228 7 0.09091254554197518 0.4955055669265595 8 0.058980256558219736 0.36738661049479054 9 0.047009048810739515 0.3294205235293983 >10 0.19821447652994786 2.684604754619967 >50 0.01312954212237831 0.7091962398710142 >100 0.011087717632749497 1.7479563909148936 >500 0.0011441220309483281 0.6488088901821483 >1k 7.865838962769732E-4 1.226295014696798 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4197 0.23109960905236493 No Hit GGTATCAACGCAGAGTACTTTTTTT 2538 0.1397500137657618 No Hit TATCAACGCAGAGTACTTTTTTTTT 2512 0.138318374538847 No Hit GTCCTACAGTGGACATTTCTAAATT 2209 0.12163427124057045 No Hit CTGTAGGACGTGGAATATGGCAAGA 1991 0.1096305269533616 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1850 0.10186663729970817 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.506304718903144E-5 0.0 8 0.0 0.0 0.0 1.6518914156709433E-4 0.0 9 0.0 0.0 0.0 1.6518914156709433E-4 0.0 10 0.0 0.0 0.0 1.6518914156709433E-4 0.0 11 0.0 0.0 0.0 1.6518914156709433E-4 0.0 12 0.0 0.0 0.0 1.6518914156709433E-4 0.0 13 0.0 0.0 0.0 1.6518914156709433E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTATT 40 0.004276494 14.709438 1 GTCAACG 55 1.4973962E-4 14.263699 2 TGGACCG 55 2.0227137E-4 13.767324 5 TAGGACC 335 0.0 12.996764 4 TCCAACG 90 7.7702225E-6 11.569014 18 CCAACGA 60 0.00603731 11.043149 19 AATCCCG 130 4.037738E-7 10.193677 19 TTAGGAC 855 0.0 10.184598 3 AGGACGT 1340 0.0 10.100746 5 GGCGAGG 630 0.0 10.066544 19 CTAGTAC 105 4.2749383E-5 9.9157505 3 CCTAGAC 125 2.693865E-6 9.8436365 3 TCTATAC 280 0.0 9.803072 3 ATTACAC 205 4.3655746E-11 9.69589 3 GGACGTG 1340 0.0 9.606304 6 GACGTGG 695 0.0 9.533129 7 GTCTAAG 155 2.6884663E-7 9.489961 1 TAATACT 190 1.1241355E-9 9.4650345 4 CTAGTAG 90 0.0011518945 9.4650345 3 GTAGGAC 1385 0.0 9.362526 3 >>END_MODULE