FastQCFastQC Report
Thu 26 May 2016
SRR1511981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816100
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53030.29199933924343374No Hit
TATCAACGCAGAGTACTTTTTTTTT31640.1742194813060955No Hit
GGTATCAACGCAGAGTACTTTTTTT30540.16816254611530201No Hit
GTCCTACAGTGGACATTTCTAAATT25980.14305379659710368No Hit
GTCCTAAAGTGTGTATTTCTCATTT22080.12157920819338142No Hit
CTGTAGGACGTGGAATATGGCAAGA21570.11877099278674083No Hit
CTTTAGGACGTGAAATATGGCGAGG21230.11689884918231375No Hit
GTACATAAGCAGTGGTATCAACGCA18570.10225207863003138No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT550.00307752712.08631919
GCGCCAC550.003080066512.08498813
AGGACCG802.8555189E-511.879655
GGCTGCG658.05761E-411.6865817
AAAGGCG752.0666522E-411.4044645
AGCACCG600.00585920311.0876735
CTAATAC1909.094947E-1210.5041113
TAGGACC4900.010.4734884
GGCGAGG5850.010.38921619
GTATTAG1201.4340676E-610.3404321
CTTATAC2053.6379788E-1210.1991143
GGTATCA14850.09.8985331
TCGTCAA1356.4856795E-79.84702710
TTAGGAC9450.09.7551413
GTTTAAC1106.4917374E-59.5450141
CCTGTAC2600.09.5037193
TGCCGGT1106.873116E-59.49534810
AAGTCGT1401.0655149E-69.4953487
GCCGGTT900.00111870449.49534711
GTAGGAC13000.09.2844043
Produced by FastQC (version 0.11.5)