FastQCFastQC Report
Thu 26 May 2016
SRR1511980_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511980_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649750
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39500.2394302166994999No Hit
TATCAACGCAGAGTACTTTTTTTTT24670.14953780875890285No Hit
GGTATCAACGCAGAGTACTTTTTTT24080.1459615093195939No Hit
CTGTAGGACGTGGAATATGGCAAGA20790.12601909380209123No Hit
GTCCTACAGTGGACATTTCTAAATT20690.1254129413547507No Hit
CTTTAGGACGTGAAATATGGCGAGG20300.12304894681012274No Hit
GTCCTAAAGTGTGTATTTCTCATTT19920.12074556751022882No Hit
GTACATAAGCAGTGGTATCAACGCA19240.11662373086831339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC350.002225021116.2250828
TCCAACG1501.8189894E-1212.62027418
GGACCGC600.00604130811.0420696
CCAACGA1159.242358E-710.69979719
TAGGACC3900.010.6780464
CTCAACG650.00839429610.5637472
CGTTAGG650.00839429610.5637471
ACCTAAT1251.7051425E-610.2015611
CCTGTAC2053.6379788E-1210.1571663
GGCGAGG6250.010.14669919
AAGTCGT1401.0228359E-710.14067657
CGGTTTC856.8195624E-410.02198113
TTAGTAC856.823363E-410.0213753
GACCTAC1104.8122467E-59.8091951
GTATAAG2251.4551915E-119.5912111
GGACGTG14550.09.5622046
AGGACGT14850.09.5602335
GTATTAA1751.5828846E-89.5289321
GTATTAG1552.6761882E-79.4927681
CGAAATC1201.7754795E-59.46520513
Produced by FastQC (version 0.11.5)