FastQCFastQC Report
Thu 26 May 2016
SRR1511980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649750
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46680.28295196241854825No Hit
GGTATCAACGCAGAGTACTTTTTTT30540.18511895741779058No Hit
TATCAACGCAGAGTACTTTTTTTTT29620.17954235490225792No Hit
CTTTAGGACGTGAAATATGGCGAGG24100.14608273980906197No Hit
CTGTAGGACGTGGAATATGGCAAGA23930.1450522806485831No Hit
GTCCTACAGTGGACATTTCTAAATT23850.14456735869071072No Hit
GTCCTAAAGTGTGTATTTCTCATTT23090.13996060009092287No Hit
GTACATAAGCAGTGGTATCAACGCA22650.13729352932262465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT456.749393E-414.78164
TTTACGT500.001496696613.3034393
ATCCCGT905.436468E-712.66072710
GGCGAGG7250.011.52807219
CCAACGA1201.2797318E-711.08048619
CTCGAAA600.00589513811.07847118
GATATAC4450.010.9422281
TCCAACG1502.1482265E-910.76194318
TAGGACC5000.010.6427524
AATCCCG2250.010.55284419
ACTGTTC4650.010.4144688
TGGCGAG14100.010.37133418
AGGACGT16600.010.1321375
GTAGGAC14300.010.1005133
TAAGCCC951.6396768E-410.0025864
GGACGTG16300.09.9646046
CCGTTTC1252.5848058E-69.87536613
CCCGTTT1356.482278E-79.84723112
TGTAGGA14900.09.8213312
TAGGACG17850.09.6887794
Produced by FastQC (version 0.11.5)