FastQCFastQC Report
Thu 26 May 2016
SRR1511979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776595
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT20560.11572699461610554No Hit
CTGTAGGACGTGGAATATGGCAAGA19450.10947908780560567No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACCG500.001541774813.2512395
TAGGACC5500.011.53034
CTACACG600.00603905311.04274
ACCGTCC600.00603905311.04278
TCTACGC700.001537984510.8173393
CCATTAG1001.6914842E-510.785671
GTCCTAA6500.010.7102461
TTAGACT1356.002119E-810.5168564
TGTAGGA14100.010.5005562
GTAGGAC14400.010.3853963
ATAGATC1201.5971837E-610.2539353
GGCGAGG3350.010.172119
GACGTGG6050.010.1691927
CGGTTTC750.00272915510.09646713
ACACCGT750.00272979210.0961836
ACGAAAT750.00272979210.09618312
CTAGCAC1701.6607373E-910.0219463
GCCGGTT951.706186E-49.96333911
CTTAGAC2002.7284841E-119.938433
CCTTATA1201.1687924E-59.8051552
Produced by FastQC (version 0.11.5)