##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511979_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1776595 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.74756148700182 31.0 30.0 34.0 25.0 34.0 2 26.753442399646516 31.0 25.0 34.0 10.0 34.0 3 28.752508590871866 31.0 28.0 34.0 16.0 34.0 4 33.293139967184416 35.0 33.0 37.0 28.0 37.0 5 33.76298593658093 35.0 35.0 37.0 28.0 37.0 6 34.04999169760131 37.0 35.0 37.0 30.0 37.0 7 34.129006892398095 37.0 35.0 37.0 30.0 37.0 8 34.175244780042725 37.0 35.0 37.0 30.0 37.0 9 35.837277488679185 39.0 37.0 39.0 30.0 39.0 10 35.742804071834044 39.0 37.0 39.0 30.0 39.0 11 35.76886065760626 39.0 37.0 39.0 30.0 39.0 12 35.65937875542822 39.0 37.0 39.0 30.0 39.0 13 35.66191112774718 39.0 37.0 39.0 30.0 39.0 14 36.900591862523534 40.0 37.0 41.0 31.0 41.0 15 36.84696681010585 40.0 37.0 41.0 30.0 41.0 16 36.788701983288256 40.0 37.0 41.0 30.0 41.0 17 36.75127927299131 40.0 37.0 41.0 30.0 41.0 18 36.70060537151123 40.0 37.0 41.0 30.0 41.0 19 36.72344062659188 40.0 37.0 41.0 30.0 41.0 20 36.626041951035546 40.0 37.0 41.0 30.0 41.0 21 36.57393215673803 40.0 37.0 41.0 30.0 41.0 22 36.474142953233574 40.0 37.0 41.0 30.0 41.0 23 36.39006920541823 40.0 37.0 41.0 30.0 41.0 24 36.298197394454 40.0 37.0 41.0 30.0 41.0 25 36.20235900697683 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.343115054654831 1101 2 4.344871826628786 1101 3 2.444027508016365 1101 4 1.5308463756437902 1101 5 1.2315301754909385 1101 6 0.7271832385199346 1101 7 0.40888155606540977 1101 8 0.24339617805571123 1101 9 0.4255614130660277 1101 10 0.5430672859529366 1101 11 0.6390351809130408 1101 12 0.5424268273525499 1101 13 0.5016212783787637 1101 14 0.24469337022359383 1101 15 0.4759323490903924 1101 16 0.5634558031688073 1101 17 0.5738295528062167 1101 18 0.8011352007048629 1101 19 0.6177051478283673 1101 20 0.6317877633976181 1101 21 0.6413324342250704 1101 22 0.7604767935714847 1101 23 0.7319728261171221 1101 24 0.6076878562326584 1101 25 0.5542749906870768 1102 1 1.7611569699471374 1102 2 4.410014243317441 1102 3 2.775748753666406 1102 4 1.8628793565504793 1102 5 1.3445230048206298 1102 6 1.2582650661770387 1102 7 1.0342175447806667 1102 8 1.0587832176908947 1102 9 1.198941272538356 1102 10 0.9862621736466082 1102 11 1.1399861443116777 1102 12 1.1524015961615603 1102 13 1.2310225454439845 1102 14 1.280928701864724 1102 15 1.3044836534613253 1102 16 1.4221580959987037 1102 17 1.3602684995890968 1102 18 1.4523140801665946 1102 19 1.356917911887308 1102 20 1.3867133513185408 1102 21 1.4720992991594102 1102 22 1.322724173153773 1102 23 1.3552288078458687 1102 24 1.4027718557846143 1102 25 1.312215031960676 1103 1 1.3024851453178634 1103 2 4.015461420253981 1103 3 2.336787714705366 1103 4 1.4288975936325485 1103 5 1.0107696659609076 1103 6 0.9614924770640272 1103 7 0.7797384068972733 1103 8 0.6471464856286318 1103 9 0.7745735834898184 1103 10 0.6743307008640684 1103 11 0.709891274642338 1103 12 0.5777714036590211 1103 13 0.7306762250976675 1103 14 0.7075824736248819 1103 15 0.8645739178069078 1103 16 0.8117574328320885 1103 17 0.9174371465449838 1103 18 0.919072853308343 1103 19 0.81660659200152 1103 20 0.8023769784715284 1103 21 0.9252299982475591 1103 22 0.978437537803309 1103 23 1.0705203248395136 1103 24 1.1266237485726762 1103 25 1.1208969883022064 1104 1 1.3922401764093841 1104 2 3.93129641120429 1104 3 2.2983811774224243 1104 4 1.4750359732895575 1104 5 1.087647969994741 1104 6 0.8422935092665966 1104 7 0.6313829421373143 1104 8 0.6477568470787318 1104 9 0.7288634962185228 1104 10 0.7639559898270321 1104 11 0.6433332042876998 1104 12 0.6792577036787364 1104 13 0.7165002847360356 1104 14 0.8501801834254294 1104 15 0.8740698230449837 1104 16 0.9800043012800188 1104 17 0.7918593102279345 1104 18 0.7262337586360132 1104 19 0.7639398894944875 1104 20 0.7022508203796676 1104 21 0.7086007847644069 1104 22 0.7287140462347566 1104 23 0.7557759562571817 1104 24 0.7933524190742602 1104 25 0.7784433567770179 1105 1 1.3585063171285086 1105 2 4.303129191079648 1105 3 2.557009598085145 1105 4 1.5702394097112062 1105 5 1.0931392262252047 1105 6 0.9071163268984037 1105 7 0.6918452474251708 1105 8 0.6069785833554633 1105 9 0.39415680728356506 1105 10 0.4484134602099914 1105 11 0.48109095215780684 1105 12 0.460933154189199 1105 13 0.5631220249118911 1105 14 0.5717572462207059 1105 15 0.6241494300140005 1105 16 0.5884354916154066 1105 17 0.7863615554693908 1105 18 0.6684813395589373 1105 19 0.7602552240712015 1105 20 0.6853806414751915 1105 21 0.7244896859282974 1105 22 0.8233456121850722 1105 23 0.6598446846901922 1105 24 0.786100061733201 1105 25 0.7680067206750962 1106 1 0.762965234897262 1106 2 2.6611623090495584 1106 3 1.3443564738907519 1106 4 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0.10382085688645049 2314 1 0.5608717881946959 2314 2 2.3670847865159317 2314 3 1.3761453891037299 2314 4 0.7422436922443225 2314 5 0.40553822275914087 2314 6 0.22824124768067833 2314 7 0.012157572222136537 2314 8 -0.04873903303333549 2314 9 -0.06929893779773266 2314 10 -0.15610230318310414 2314 11 -0.04742424532516765 2314 12 -0.1331971618428085 2314 13 -0.21940486343243748 2314 14 -0.15310162925165827 2314 15 -0.14720350215624478 2314 16 -0.27296165851218746 2314 17 -0.09624171966619599 2314 18 -0.10647220957515913 2314 19 -0.03112162361563975 2314 20 -0.10096752448043844 2314 21 -0.2697340994068256 2314 22 -0.16395740250450785 2314 23 -0.23246239851659567 2314 24 -0.09824330395354508 2314 25 -0.21364411444378106 2315 1 -2.5848679777495427 2315 2 -10.914031467810336 2315 3 -4.540021190674967 2315 4 -1.914646005625336 2315 5 -1.1326688003346987 2315 6 -0.7195464839749377 2315 7 -0.5195936895776754 2315 8 -0.5867186968079778 2315 9 -0.6401697683963903 2315 10 -0.7214392434772989 2315 11 -0.7959590654937188 2315 12 -0.640775287744809 2315 13 -0.7593299591942326 2315 14 -0.8189666914957101 2315 15 -1.1658218768745954 2315 16 -0.9918789308042761 2315 17 -0.9178627916121016 2315 18 -0.9227638813855279 2315 19 -0.8524966856731879 2315 20 -0.9146978514604456 2315 21 -0.8160130444240608 2315 22 -1.2256317262659522 2315 23 -0.9978284692235633 2315 24 -0.9193317245256551 2315 25 -0.8099378911040986 2316 1 -0.3386597269501479 2316 2 -0.14397352459368307 2316 3 0.025236478390937123 2316 4 -0.2239034889114322 2316 5 -0.4697666853738198 2316 6 -0.6490844696698872 2316 7 -0.5823282679704818 2316 8 -0.6982531125179179 2316 9 -0.7422607954825864 2316 10 -0.8490038626328769 2316 11 -0.8666478650632428 2316 12 -0.7071361290428229 2316 13 -0.8709041250889058 2316 14 -1.018049215389759 2316 15 -0.921209491386513 2316 16 -1.2029340011286322 2316 17 -1.1913084966255596 2316 18 -1.259915952202789 2316 19 -1.1468099824028783 2316 20 -1.1160984387589608 2316 21 -1.144612130755604 2316 22 -1.222131617894398 2316 23 -1.2443080673366467 2316 24 -1.3121206644342536 2316 25 -1.1153870974812037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49418.0 3 6.0 4 442.0 5 2037.0 6 2348.0 7 2190.0 8 2074.0 9 2030.0 10 2003.0 11 1912.0 12 1929.0 13 1971.0 14 2034.0 15 2045.0 16 2155.0 17 2286.0 18 2627.0 19 3426.0 20 4308.0 21 5597.0 22 6929.0 23 8071.0 24 9512.0 25 11057.0 26 12657.0 27 14325.0 28 16899.0 29 20350.0 30 26284.0 31 34150.0 32 44521.0 33 60173.0 34 84974.0 35 125114.0 36 205864.0 37 286283.0 38 716594.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.04824960106271 18.19294774554696 11.697882747615523 26.06091990577481 2 17.56957369493929 21.2703554952257 38.081918769270956 23.078152040564053 3 19.117300228808478 23.738105758487443 29.28016796174705 27.864426050957025 4 12.233570397304957 16.06229894826902 38.105871062341166 33.59825959208486 5 13.235205547690947 37.56708760297085 35.96869292100901 13.229013928329191 6 32.66733986493256 36.794225354808134 17.82770852541436 12.710726254844944 7 28.700857539281603 31.18116396815256 21.958015191982415 18.15996330058342 8 25.48978551092709 36.94434406510435 20.279985185135153 17.28588523883341 9 26.526360819432675 15.128490173618637 19.444893180494148 38.90025582645454 10 14.141569927141404 29.19032619757378 34.975953961292184 21.692149913992633 11 35.4865346350744 22.03704277001793 23.887492647452007 18.588929947455668 12 22.900492233739257 24.301655695304785 31.99626251340345 20.801589557552507 13 29.29322664985548 21.272321491392244 25.63521793092967 23.799233927822605 14 20.997638741525222 21.162279529099205 27.49045224150693 30.349629487868647 15 23.20799056622359 30.227879736237018 23.022241985370894 23.541887712168503 16 22.748684984478736 27.852155387131 26.97863047008463 22.420529158305634 17 22.107176931152004 28.12711957424174 27.50621272715503 22.259490767451222 18 22.00072385985038 27.540165497102027 28.890451185334577 21.568659457713018 19 23.599016984375545 26.9234492906009 27.005235920372584 22.472297804650974 20 23.45775396674849 27.239681162862823 27.625590952509345 21.67697391787934 21 23.208016495012487 26.794657246091695 26.724239348800506 23.273086910095316 22 23.053221130843486 27.57186682052292 27.091779009878696 22.28313303875489 23 22.811866229945217 27.223060603826134 27.104573923586507 22.860499242642145 24 22.99281659174803 26.70852203711146 27.82211354986001 22.476547821280494 25 22.99795111956681 27.11715768499026 27.427810737484382 22.45708045795855 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 192.0 1 192.0 2 234.5 3 277.0 4 277.0 5 277.0 6 670.5 7 1064.0 8 1064.0 9 1064.0 10 1707.0 11 2350.0 12 2350.0 13 2350.0 14 4308.0 15 6266.0 16 6266.0 17 6266.0 18 11837.0 19 17408.0 20 17408.0 21 17408.0 22 27882.0 23 38356.0 24 38356.0 25 38356.0 26 55553.5 27 72751.0 28 72751.0 29 72751.0 30 95690.5 31 118630.0 32 118630.0 33 118630.0 34 138411.5 35 158193.0 36 158193.0 37 158193.0 38 175708.0 39 193223.0 40 193223.0 41 193223.0 42 209871.0 43 226519.0 44 226519.0 45 226519.0 46 231274.5 47 236030.0 48 236030.0 49 236030.0 50 230702.5 51 225375.0 52 225375.0 53 225375.0 54 204461.0 55 183547.0 56 183547.0 57 183547.0 58 158751.5 59 133956.0 60 133956.0 61 133956.0 62 109040.5 63 84125.0 64 84125.0 65 84125.0 66 65193.0 67 46261.0 68 46261.0 69 46261.0 70 33391.5 71 20522.0 72 20522.0 73 20522.0 74 14180.5 75 7839.0 76 7839.0 77 7839.0 78 5195.0 79 2551.0 80 2551.0 81 2551.0 82 1681.5 83 812.0 84 812.0 85 812.0 86 530.5 87 249.0 88 249.0 89 249.0 90 156.0 91 63.0 92 63.0 93 63.0 94 42.5 95 22.0 96 22.0 97 22.0 98 18.0 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.466124806160099 3 0.0 4 0.0 5 0.0 6 5.6287448743241986E-5 7 0.0 8 5.6287448743241986E-5 9 0.0 10 1.6886234622972597E-4 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.502995899459359E-4 19 0.0011820364236080818 20 7.880242824053878E-4 21 0.003264672027108035 22 0.0025329351934458896 23 0.0013508987698378078 24 5.6287448743241986E-5 25 8.443117311486299E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1776595.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.78234362568612 #Duplication Level Percentage of deduplicated Percentage of total 1 83.9727232977242 63.636497721327935 2 11.102618714870252 16.827649331905423 3 2.7799176156200516 6.320060159940503 4 0.9802409832121644 2.9713983610305865 5 0.4305398831402414 1.631366068434826 6 0.216621546099135 0.9849653065927235 7 0.12593992918479907 0.6680816092773914 8 0.08854327101807952 0.5368013272027485 9 0.052289292917212384 0.35663446474167093 >10 0.2288547278915246 2.9055457428328357 >50 0.011138863064201776 0.588004408552195 >100 0.008997806105581072 1.3968859381014453 >500 0.001049379435079783 0.5577502907619798 >1k 5.246897175398906E-4 0.6183592692977802 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2056 0.11572699461610554 No Hit CTGTAGGACGTGGAATATGGCAAGA 1945 0.10947908780560567 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.6287448743241986E-5 0.0 5 0.0 0.0 0.0 5.6287448743241986E-5 0.0 6 0.0 0.0 0.0 5.6287448743241986E-5 0.0 7 0.0 0.0 0.0 5.6287448743241986E-5 0.0 8 0.0 0.0 0.0 5.6287448743241986E-5 0.0 9 0.0 0.0 0.0 1.1257489748648397E-4 0.0 10 0.0 0.0 0.0 2.2514979497296794E-4 0.0 11 0.0 0.0 0.0 2.2514979497296794E-4 0.0 12 0.0 0.0 0.0 2.2514979497296794E-4 0.0 13 0.0 0.0 0.0 2.2514979497296794E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACACCG 50 0.0015417748 13.251239 5 TAGGACC 550 0.0 11.5303 4 CTACACG 60 0.006039053 11.0427 4 ACCGTCC 60 0.006039053 11.0427 8 TCTACGC 70 0.0015379845 10.817339 3 CCATTAG 100 1.6914842E-5 10.78567 1 GTCCTAA 650 0.0 10.710246 1 TTAGACT 135 6.002119E-8 10.516856 4 TGTAGGA 1410 0.0 10.500556 2 GTAGGAC 1440 0.0 10.385396 3 ATAGATC 120 1.5971837E-6 10.253935 3 GGCGAGG 335 0.0 10.1721 19 GACGTGG 605 0.0 10.169192 7 CGGTTTC 75 0.002729155 10.096467 13 ACACCGT 75 0.002729792 10.096183 6 ACGAAAT 75 0.002729792 10.096183 12 CTAGCAC 170 1.6607373E-9 10.021946 3 GCCGGTT 95 1.706186E-4 9.963339 11 CTTAGAC 200 2.7284841E-11 9.93843 3 CCTTATA 120 1.1687924E-5 9.805155 2 >>END_MODULE