FastQCFastQC Report
Thu 26 May 2016
SRR1511979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776595
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT22320.1256335855949161No Hit
CTGTAGGACGTGGAATATGGCAAGA20850.11735933062965954No Hit
GTATCAACGCAGAGTACTTTTTTTT20330.11443238329501096No Hit
GTCCTAAAGTGTGTATTTCTCATTT17880.10064195835291667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC1103.8402504E-812.0851258
TGTACCG752.0660092E-411.4048185
TAGGACC4550.011.27948954
TCAAGAC2150.011.0511793
CTAGGAC2850.011.0046473
TATCCGT700.001491934210.8568376
TCGTCAA803.78796E-410.68238610
GGCGAGG3750.010.63640619
GTCCTAC12100.010.567081
ACACCGT1002.3996472E-510.4497066
CTTACAC2200.010.3680163
CCTACAC2800.010.1828723
AAATGTC3550.010.1641497
GGACCGT750.002648507210.1330486
AGGACCT8350.010.1300275
AATGTCC3900.09.9824018
GCGCCAC1054.12675E-59.947618513
GTATTAG1553.7949576E-89.8497181
TCCTACA13350.09.7531822
CTAATAC2054.0017767E-119.7358193
Produced by FastQC (version 0.11.5)