##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511979_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1776595 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.434715284012395 33.0 31.0 34.0 28.0 34.0 2 31.60732637432842 33.0 31.0 34.0 28.0 34.0 3 31.73606139834909 34.0 31.0 34.0 28.0 34.0 4 35.25907311458155 37.0 35.0 37.0 32.0 37.0 5 34.97725818208427 37.0 35.0 37.0 32.0 37.0 6 34.933852678860404 37.0 35.0 37.0 32.0 37.0 7 34.81269113106814 37.0 35.0 37.0 32.0 37.0 8 34.74082781950867 37.0 35.0 37.0 32.0 37.0 9 36.33976511247639 39.0 37.0 39.0 32.0 39.0 10 36.16453046417445 39.0 37.0 39.0 32.0 39.0 11 36.18324097501119 39.0 37.0 39.0 32.0 39.0 12 36.016666150698384 39.0 37.0 39.0 31.0 39.0 13 36.00745414683707 39.0 37.0 39.0 31.0 39.0 14 37.14130288557606 40.0 37.0 41.0 31.0 41.0 15 37.09440193178524 40.0 37.0 41.0 31.0 41.0 16 36.9965827889868 40.0 37.0 41.0 31.0 41.0 17 36.91531891061272 40.0 37.0 41.0 31.0 41.0 18 36.84062884337736 40.0 37.0 41.0 31.0 41.0 19 36.8583852819579 40.0 37.0 41.0 31.0 41.0 20 36.74703351073261 40.0 37.0 41.0 30.0 41.0 21 36.643789383624295 40.0 37.0 41.0 30.0 41.0 22 36.52836352685896 40.0 37.0 41.0 30.0 41.0 23 36.648784331825766 40.0 37.0 41.0 31.0 41.0 24 36.57713716406947 40.0 37.0 41.0 30.0 41.0 25 36.1192567805268 39.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23584581867128307 1101 2 0.317737634954387 1101 3 0.3113503943062277 1101 4 0.30307900559727585 1101 5 0.42751863270409984 1101 6 0.4239192433678127 1101 7 0.24365406021118474 1101 8 0.38004820222897706 1101 9 0.22473347666416288 1101 10 0.511851744004197 1101 11 0.38407165715275227 1101 12 0.5435904273709511 1101 13 0.4208068741540032 1101 14 0.36563466578379433 1101 15 0.31356347238464366 1101 16 0.5413555080503514 1101 17 0.5386491592905145 1101 18 0.578591943956809 1101 19 0.5989135080605621 1101 20 0.49146114245687045 1101 21 0.3835695346405217 1101 22 0.39323161968236775 1101 23 0.3245622053481654 1101 24 0.2026097406410372 1101 25 0.25016709745308674 1102 1 0.42686055300335823 1102 2 0.3871592920114324 1102 3 0.2553424661306387 1102 4 0.37019966916431457 1102 5 0.5222569136797048 1102 6 0.5948997665590525 1102 7 0.54324227350493 1102 8 0.4457301753119083 1102 9 0.4627313845067178 1102 10 0.5049639516100015 1102 11 0.49915626422773585 1102 12 0.7696307631033648 1102 13 0.6205517144798662 1102 14 0.7851486853270515 1102 15 0.8055192978660557 1102 16 0.9007938530057302 1102 17 0.8685482155414519 1102 18 0.9423368678170903 1102 19 0.9516558100792452 1102 20 0.9537850319712078 1102 21 0.8306313064434789 1102 22 0.7728906094095294 1102 23 0.9118027028335902 1102 24 1.0427298023805704 1102 25 1.0515890810537627 1103 1 0.1399194090409388 1103 2 0.14952458629161924 1103 3 0.28317520077188973 1103 4 0.2918704806223573 1103 5 0.20065057675422082 1103 6 0.22474410661615707 1103 7 0.1735618829734733 1103 8 0.20141590801891596 1103 9 0.22382192517172683 1103 10 0.23131192987287363 1103 11 0.4709090504486113 1103 12 0.4738105549427303 1103 13 0.38096435148824526 1103 14 0.3385262297684264 1103 15 0.4256998265572278 1103 16 0.48641787437443185 1103 17 0.4138903910963947 1103 18 0.35011618070278416 1103 19 0.59575639035004 1103 20 0.2806156481635256 1103 21 0.24909865469805936 1103 22 0.36033097046691154 1103 23 0.31480353179186693 1103 24 0.31103780643325507 1103 25 0.4769662570692361 1104 1 0.07206069176767116 1104 2 0.08266796567982482 1104 3 0.08993791962212683 1104 4 0.21180991040211694 1104 5 0.1825855981688349 1104 6 0.26352015808292606 1104 7 0.3278973129682754 1104 8 0.3426606439650115 1104 9 0.3553123468336423 1104 10 0.458578564283215 1104 11 0.3973273433719271 1104 12 0.3635096750617208 1104 13 0.36653860376858916 1104 14 0.5076035195198756 1104 15 0.6054840675478559 1104 16 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-0.4137913021200532 2314 17 -0.31558985898701764 2314 18 -0.25621510728480246 2314 19 -0.30396074268447393 2314 20 -0.6058981252817901 2314 21 -0.6114769433117146 2314 22 -0.6297126925877592 2314 23 -0.6471817591259281 2314 24 -0.5074431254320757 2314 25 -0.5004148300102713 2315 1 -0.7624182532967261 2315 2 -0.7501043561944662 2315 3 -1.0209868276758556 2315 4 -0.7566329207381841 2315 5 -0.97443909547831 2315 6 -0.9425533578241598 2315 7 -1.0122327985354929 2315 8 -0.9403156937126838 2315 9 -1.025349379713866 2315 10 -1.1352681567072125 2315 11 -1.0879159340906597 2315 12 -1.084896928050469 2315 13 -1.0924431933478687 2315 14 -1.2512820571827206 2315 15 -1.2114368462937293 2315 16 -1.1643366092371963 2315 17 -1.2737286565226498 2315 18 -1.2253692862112544 2315 19 -1.2648187487965288 2315 20 -1.237380641261332 2315 21 -1.3690213205647694 2315 22 -1.5481353251422405 2315 23 -1.4660127947387451 2315 24 -1.3711947229421355 2315 25 -1.4559465444149922 2316 1 -0.9716105044375567 2316 2 -0.9124018499243967 2316 3 -0.9181771590919752 2316 4 -0.8070748591078285 2316 5 -0.8774779343377546 2316 6 -1.0593096925391308 2316 7 -1.252278933855166 2316 8 -1.137472844185126 2316 9 -1.2416211387557752 2316 10 -1.386942057907497 2316 11 -1.4299118787349698 2316 12 -1.6166671585931667 2316 13 -1.4113430253928811 2316 14 -1.8046820369310481 2316 15 -1.5837109250727437 2316 16 -1.6206358837754067 2316 17 -1.5792690791131463 2316 18 -1.6505259647025596 2316 19 -1.6583462052951816 2316 20 -1.6838572267288328 2316 21 -1.5668893717094505 2316 22 -1.6633554198229916 2316 23 -1.628009442333692 2316 24 -1.665696519232064 2316 25 -1.67732578377219 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18239.0 3 0.0 4 312.0 5 2975.0 6 4021.0 7 3300.0 8 2636.0 9 2522.0 10 2344.0 11 2231.0 12 2173.0 13 2115.0 14 2056.0 15 2036.0 16 2200.0 17 2283.0 18 2455.0 19 2708.0 20 3219.0 21 4131.0 22 5338.0 23 6927.0 24 8257.0 25 10092.0 26 11671.0 27 13483.0 28 15555.0 29 19467.0 30 24650.0 31 32688.0 32 42747.0 33 56209.0 34 76449.0 35 105267.0 36 161833.0 37 316975.0 38 805031.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54670871285941 18.66544434101302 12.096499631831893 25.69134731429567 2 15.63952392075853 20.40763370379856 39.70747412888137 24.24536824656154 3 19.22492183080556 24.060407689991248 29.664329799419676 27.05034067978352 4 12.643511886501987 16.478882356417753 37.835466158578626 33.04213959850163 5 13.1775913544 37.79054511381924 35.98631894164377 13.045544590136979 6 32.70958509582055 37.12939899068686 17.586839394637384 12.574176518855209 7 28.7824743399593 31.228445425096886 21.963024774920566 18.026055460023247 8 25.608199955532918 37.099282616465764 20.12895454507077 17.16356288293055 9 26.49078715182695 15.040006304194259 19.42029556539335 39.04891097858544 10 14.03910289064193 29.386213515179314 35.04529732437612 21.52938626980263 11 35.77562697181969 21.913266670231536 23.781841106160943 18.52926525178783 12 22.840320951032734 24.40353597753005 31.981008614794032 20.775134456643187 13 29.37636321164925 21.276430475150498 25.654580813297343 23.692625499902903 14 20.96133333708583 21.10216453384142 27.55450735817674 30.38199477089601 15 23.240468424148442 30.307864200901165 23.020722224254826 23.430945150695575 16 22.748009535093814 27.956512317100973 26.898195705830535 22.397282441974674 17 22.121867955273995 28.22410284842634 27.400842623107685 22.25318657319198 18 21.997022393961483 27.54032292109344 28.85395939986322 21.608695285081854 19 23.61410450890608 26.91840289992936 27.046119121127777 22.421373470036784 20 23.593192137985458 27.216813305016963 27.596979161800146 21.593015395197437 21 23.26461573965929 26.73423036764147 26.76895972351605 23.232194169183185 22 23.146243234952255 27.53705824906633 27.074431707845626 22.24226680813579 23 22.81926944520276 27.220947936924283 27.1691634840805 22.79061913379245 24 22.988826013755975 26.795220138955735 27.86375966275963 22.352194184528663 25 23.07742106181638 27.16802053603094 27.355141607417288 22.39941679473539 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 171.0 1 171.0 2 212.0 3 253.0 4 253.0 5 253.0 6 659.5 7 1066.0 8 1066.0 9 1066.0 10 1694.0 11 2322.0 12 2322.0 13 2322.0 14 4184.0 15 6046.0 16 6046.0 17 6046.0 18 11658.0 19 17270.0 20 17270.0 21 17270.0 22 27939.0 23 38608.0 24 38608.0 25 38608.0 26 56155.0 27 73702.0 28 73702.0 29 73702.0 30 96641.5 31 119581.0 32 119581.0 33 119581.0 34 139543.0 35 159505.0 36 159505.0 37 159505.0 38 176610.5 39 193716.0 40 193716.0 41 193716.0 42 210568.0 43 227420.0 44 227420.0 45 227420.0 46 231717.5 47 236015.0 48 236015.0 49 236015.0 50 231141.0 51 226267.0 52 226267.0 53 226267.0 54 204580.0 55 182893.0 56 182893.0 57 182893.0 58 157902.0 59 132911.0 60 132911.0 61 132911.0 62 107949.0 63 82987.0 64 82987.0 65 82987.0 66 64091.5 67 45196.0 68 45196.0 69 45196.0 70 32433.5 71 19671.0 72 19671.0 73 19671.0 74 13671.0 75 7671.0 76 7671.0 77 7671.0 78 5053.5 79 2436.0 80 2436.0 81 2436.0 82 1558.5 83 681.0 84 681.0 85 681.0 86 425.5 87 170.0 88 170.0 89 170.0 90 100.0 91 30.0 92 30.0 93 30.0 94 18.5 95 7.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47174510791711105 2 0.0 3 0.0 4 0.0 5 0.03990780115895857 6 0.05245990222870154 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.1257489748648397E-4 21 0.0 22 0.0 23 0.0 24 0.003264672027108035 25 0.011538926992364607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1776595.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.33709858802136 #Duplication Level Percentage of deduplicated Percentage of total 1 82.32060446272986 60.37154285308731 2 12.076981802356132 17.71381610170263 3 3.1147430608273234 6.852786567847466 4 1.1159118604120821 3.2735095249033277 5 0.5000026790858236 1.8334372885195929 6 0.2555681890084485 1.12455776839648 7 0.15722307424542595 0.8071198867374023 8 0.10358570070215477 0.6077339795762559 9 0.06732855798827567 0.44439129854779374 >10 0.26185364934334043 3.199403349980459 >50 0.014067166709975295 0.7125823538633513 >100 0.009980769970299369 1.5037988419821207 >500 0.001304766162619446 0.6126268417766064 >1k 8.442604581655201E-4 0.9426933430792509 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2232 0.1256335855949161 No Hit CTGTAGGACGTGGAATATGGCAAGA 2085 0.11735933062965954 No Hit GTATCAACGCAGAGTACTTTTTTTT 2033 0.11443238329501096 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1788 0.10064195835291667 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1257489748648397E-4 2 0.0 0.0 0.0 0.0 1.1257489748648397E-4 3 0.0 0.0 0.0 0.0 1.1257489748648397E-4 4 0.0 0.0 0.0 0.0 1.1257489748648397E-4 5 0.0 0.0 0.0 0.0 1.1257489748648397E-4 6 0.0 0.0 0.0 0.0 1.1257489748648397E-4 7 0.0 0.0 0.0 0.0 1.1257489748648397E-4 8 0.0 0.0 0.0 0.0 1.1257489748648397E-4 9 0.0 0.0 0.0 0.0 1.1257489748648397E-4 10 0.0 0.0 0.0 1.1257489748648397E-4 1.1257489748648397E-4 11 0.0 0.0 0.0 1.6886234622972597E-4 1.1257489748648397E-4 12 0.0 0.0 0.0 1.6886234622972597E-4 1.1257489748648397E-4 13 0.0 0.0 0.0 1.6886234622972597E-4 1.1257489748648397E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTGC 110 3.8402504E-8 12.085125 8 TGTACCG 75 2.0660092E-4 11.404818 5 TAGGACC 455 0.0 11.2794895 4 TCAAGAC 215 0.0 11.051179 3 CTAGGAC 285 0.0 11.004647 3 TATCCGT 70 0.0014919342 10.856837 6 TCGTCAA 80 3.78796E-4 10.682386 10 GGCGAGG 375 0.0 10.636406 19 GTCCTAC 1210 0.0 10.56708 1 ACACCGT 100 2.3996472E-5 10.449706 6 CTTACAC 220 0.0 10.368016 3 CCTACAC 280 0.0 10.182872 3 AAATGTC 355 0.0 10.164149 7 GGACCGT 75 0.0026485072 10.133048 6 AGGACCT 835 0.0 10.130027 5 AATGTCC 390 0.0 9.982401 8 GCGCCAC 105 4.12675E-5 9.9476185 13 GTATTAG 155 3.7949576E-8 9.849718 1 TCCTACA 1335 0.0 9.753182 2 CTAATAC 205 4.0017767E-11 9.735819 3 >>END_MODULE